DO NOW!! What is the difference between an autotroph and a heterotroph? Why do plants need sunlight? What gases are exchanged between plants and animals? Overview Of Photosynthesis Objectives To be able to list the inputs and outputs of photosynthesis. To be able to identify the structures in a chloroplast. To be able to summarize lightdependent reactions. Video intro https://www.youtube.com/watch?v=eo5XndJaz-Y Photosynthesis Process of converting light energy to chemical energy!! Used by autotrophs to produce food All of our energy starts as light energy! Plants use sunlight to make food animals eat plants other animals eat those animals Photosynthetic Organisms Go through Photosynthesis to produce GLUCOSE… Autotrophs “Producers” in the food web Consist of plants, protists, cyanobacteria Photosynthesis: The Chemical Equation Overview of Phases 1) Light dependent Light energy is absorbed and converted into chemical energy in the form of ATP and NADPH. (also produces oxygen as byproduct!) 2) Light independent (Calvin Cycle) CO2 ATP and NADPH (from light dependent) are used to make glucose. *Glucose is the basic building block for more complex sugars such as starch.* Glucose The Chloroplast Structures within a chloroplast Thylakoids: flattened sac-like membranes arranged in stacks (stacks are called grana). Light-dependent reactions take place here. Electron transport occurs in the thylakoid membrane Structures within a chloroplast (cont.) Stroma: Aqueous space outside the grana. Light-independent reactions take place here. Light Dependent Reactions Step 1: Light energy reaches photosytem II, exciting electrons and causing water molecule to split. - H+ is released, stays in thylakoid space. - O2 is given off as a byproduct. - Electron released into electron transport system Light Dependent Reactions Step 2: Excited electrons move from photosystem II through the membrane As they move, protons (H+) are pumped into thylakoid space (**active transport!! Tada!!) Light Dependent Reaction Step 3: At photosystem I, electrons are re-energized and transferred to ferrodoxin (helper protein): NADPH (energy storage molecule) is formed from NADP+ (we need this for the Calvin Cycle!) Light Dependent Reactions Step 4: Hydrogen ions move through the ATP Synthase because of the concentration gradient, creating ATP from ADP Light Dependent Reactions http://www.science.smith.edu/departments/Biology/Bio231/ltrxn.html https://www.youtube.com/watch?v=joZ1EsA5_NY Do Now!! What goes into a light dependent reaction? What comes out? What is the goal of a light dependent reaction? What is given off as a byproduct? What is the name of the protein that converts NADP+ to NADPH (the last electron acceptor)? Objectives To review light dependent reactions To introduce light independent reactions by completing a Calvin Cycle activity The Calvin Cycle Takes place in the STROMA Uses NADPH and ATP from Phase I (light dependent reactions) Needs CO2!!! Produces GLUCOSE!! Calvin Cycle Functions like a sugar factory within a chloroplast Regenerates the starting material with each turn Calvin Cycle Inputs Outputs - ATP - Glucose - NADPH - NADP+ - CO2 - ADP Calvin Cycle Do Now!! How does our body make energy? What are the outputs of photosynthesis? What do you think the outputs of cellular respiration are? Objectives To compare and contrast photosynthesis and cellular respiration To identify the inputs and outputs of cellular respiration To define NADH and FADH2 So, what is cellular respiration? How does cellular respiration compare to photosynthesis? Inputs Outputs Chemical Formula Photosynthesis Cell Respiration CO2 + H2O + light O2 + C6H12O6 O2 + C6H12O6 CO2 + H2O + ATP CO2 + H2O + Light O2 + C6H12O6 O2 + C6H12O6 CO2 + H2O + ATP All cells require energy to do work o Aerobic = NEEDS OXYGEN!! o In aerobic respiration we use oxygen to help release the energy stored in bonds. o Anaerobic = does NOT require oxygen NADH and FADH2 FADH2 (Riboflavin) FADH2 FAD + 2 H+ + 2e- B2 Vitamin NADH (Niacin) NADH NAD+ + H+ + 2e- B3 Vitamin *These are electron carriers! Steps of Cellular Respiration Step 1: Glycolysis (anaerobic, cytoplasm) Step 2: Krebs Cycle (aerobic, mitochondrial matrix) Step 3: Electron Transport Chain (aerobic, mitochondrial membrane) Aerobic vs. Anaerobic Respiration Cytoplasm Lactic acid or CO2/ethanol Steps of Cellular Respiration (cont.) 1. Glycolysis: Occurs in the cytoplasm We break glucose in half (2 pyruvates) We make a small amount of ATP (2) (we made 4 total, but had to use two ATP to break down glucose) Make NADH (used to make ATP later on) Inputs Outputs 4 ADP 2 ATP (net) 2 NAD+ 2 NADH 1 glucose 2 Pyruvates Steps of Cellular Respiration (cont) 2. Krebs Cycle (Citric Acid Cycle) Occurs in mitochondrial matrix We take the broken glucose (pyruvates) and make more ATP, NADH, and another molecule called FADH2. We also release CO2 Inputs Outputs 2 Pyruvates 6 CO2 NAD+ NADH 2 ADP 2 ATP FAD FADH2 Steps of Cellular Respiration (cont) 3. Electron Transport Chain Occurs in the mitochondrial membrane Takes NADH and FADH2 and makes a lot of ATP (32)!! Uses oxygen as a final electron acceptor to make water Input Output 6 O2 6 H2O 32 ADP 32 ATP NADH NAD+ FADH2 FAD Do Now!! Where does the electron transport chain take place? How many ATP are produced? How many total molecules of ATP are produced for one molecule of glucose? Objectives To define anaerobic respiration. To compare and contrast alcohol fermentation and lactic acid fermentation. Stand up! Move your arms up and down for one minute! Do not stop until the time is up! What is happening in your arms? Anaerobic Respiration Making ATP without oxygen (fermentation) Problems: Only 2 net ATP per glucose molecule Limited amount of NAD+ Yeast, People, Bacteria, Fungus Used to make beer, wine, and bread 2 Main Types of Fermentation 1. Lactic Acid Fermentation Pyruvate from glycolysis converted to lactic acid Lactic acid is associated with the “burn” associated with heavy exercise Lactic Acid Fermentation Inputs: Glucose, 2ADP Outputs: 2ATP, 2 lactic acid Ex: Bacteria that help in making yogurt, cheese and sour cream. Human muscle cells when out of oxygen. Lactic Acid Fermentation Physical conditioning allows your body to adapt to increased activity: The body can increase its ability to deliver oxygen to muscles Long-distance runners wait until the final sprint to exceed their aerobic capacity 2 Main Types of Fermentation (cont.) 2. Alcohol Fermentation In yeast and some bacteria Pyruvate is converted to ethyl alcohol and carbon dioxide Ex: yeast used for baking bread and making wine/beer https://www.youtube.com/wat ch?v=i1w09QKf9GU Alcohol Fermentation Inputs: Glucose, 2ADP Outputs: 2ATP, 2 ethanol, 2 CO2 NAD+ gets regenerated Why don’t you get drunk off bread? o Take a minute and pair up with the person sitting NEXT to you and discuss this question. Aerobic vs. Anaerobic Respiration Cytoplasm Lactic acid or CO2/ethanol Do Now!! What happens when we don’t get enough oxygen? What does our body do? Do Now!! What are the two phases of photosynthesis? What are the inputs and outputs of each phase? What is the chemical equation for photosynthesis? Objectives To go over the steps of light dependent reactions To understand the inputs and outputs of light dependent reactions To label a light reactions diagram Do Now!! What is the equation for photosynthesis? Where do light dependent reactions take place? Independent? What are the inputs of light dependent reactions? The outputs? Objectives To go over the steps of light dependent reactions To understand the inputs and outputs of light dependent reactions To label a light reactions diagram