Biochemistry of Cells
1
Uses of Organic Molecules
Americans consume
an average of 140
pounds of sugar
per person per
year
Cellulose, found in
plant cell walls, is
the most abundant
organic compound on
Earth
2
Uses of Organic Molecules
A typical cell in
your body has
about 2 meters
of DNA
A typical cow
produces over
200 pounds of
methane gas
each year
3
Water
About 60-90 percent
of an organism is
water
Water is used in
most reactions in
the body
Water is called
the universal
solvent
4
Water Properties
Polarity
Cohesiveness
Adhesiveness
Surface
Tension
5
Carbon-based Molecules
Although a cell is
mostly water, the
rest of the cell
consists mostly of
carbon-based
molecules
Organic chemistry
is the study of
carbon compounds
6
Carbon is a Versatile Atom
It has four electrons
in an outer shell that
holds eight
Carbon can
share its
electrons with
other atoms to
form up to four
covalent bonds
7
Hydrocarbons
The simplest carbon
compounds …
Contain only carbon
& hydrogen atoms
8
Carbon can use its bonds to::
Attach to other
carbons
Form an
endless
diversity of
carbon
skeletons
9
Large Hydrocarbons:
Are the main
molecules in the
gasoline we burn
in our cars
The hydrocarbons
of fat molecules
provide energy for
our bodies
10
Shape of Organic Molecules
Each type of
organic molecule
has a unique
three-dimensional
shape
The shape
determines its
function in an
organism
11
Functional Groups are:
Groups of atoms that give properties to
the compounds to which they attach
Gained Electrons
Lost Electrons
12
Common Functional Groups
13
Giant Molecules - Polymers
Large molecules
are called polymers
Polymers are built
from smaller
molecules called
monomers
Biologists call
them
macromolecules
14
Examples of Polymers
Proteins
Lipids
Carbohydrates
Nucleic Acids
15
Most Macromolecules are Polymers
Polymers are made by stringing together
many smaller molecules called monomers
Nucleic Acid
Monomer
16
Linking Monomers
Cells link monomers by a process
called dehydration synthesis
(removing a molecule of water)
Remove
H
H2O Forms
Remove OH
This process joins two sugar monomers
to make a double sugar
17
Breaking Down Polymers
Cells break down
macromolecules
by a process
called
hydrolysis
(adding a
molecule of
water)
Water added to split a double sugar
18
Macromolecules in Organisms
There are four categories of large
molecules in cells:
Carbohydrates
Lipids
Proteins
Nucleic Acids
19
Carbohydrates
Carbohydrates include:
Small sugar molecules
in soft drinks
Long starch molecules
in pasta and potatoes
20
Carbohydrates
Small sugar molecules to large sugar
molecules.
Examples:
A. monosaccharide
B. disaccharide
C. polysaccharide
21
Carbohydrates
Monosaccharide: one sugar unit
Examples:
glucose
glucose (C6H12O6)
deoxyribose
ribose
Fructose
Galactose
22
Monosaccharides:
Called simple sugars
Include glucose,
fructose, & galactose
Have the same
chemical, but
different structural
formulas
C6H12O6
23
Monosaccharides
Glucose is found in
sports drinks
Fructose is found
in fruits
Honey contains
both glucose &
fructose
Galactose is called
“milk sugar”
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Isomers
Glucose &
fructose are
isomers
because
they’re
structures are
different, but
their chemical
formulas are
the same
25
Rings
In aqueous (watery) solutions,
monosaccharides form ring structures
26
Cellular Fuel
Monosaccharides
are the main
fuel that cells
use for cellular
work
ATP
27
Disaccharides
A disaccharide is a
double sugar
They’re made by
joining two
monosaccharides
Involves removing
a water molecule
(dehydration)
28
Disaccharides
Common disaccharides include:
Sucrose (table sugar)
Lactose (Milk Sugar)
Maltose (Grain sugar)
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Disaccharides
Sucrose is composed
of glucose + fructose
Maltose is
composed of 2
glucose molecules
Lactose is made
of galactose +
glucose
GLUCOSE
30
Carbohydrates
Disaccharide: two sugar unit
Examples:
Sucrose (glucose+fructose)
Lactose (glucose+galactose)
Maltose (glucose+glucose)
glucose
glucose
31
Polysaccharides
Complex
carbohydrates
Composed of many
sugar monomers
linked together
Polymers of
monosaccharide
chains
32
Examples of Polysaccharides
Glucose Monomer
Starch
Glycogen
Cellulose
33
Starch
Starch is an example of a
polysaccharide in plants
Plant cells store starch
for energy
Potatoes and grains are
major sources of starch
in the human diet
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Glycogen
Glycogen is an example
of a polysaccharide
in animals
Animals store excess
sugar in the form of
glycogen
(in muscles and liver)
Glycogen is similar in
structure to starch
35
Cellulose
Cellulose is the most abundant organic
compound on Earth
It forms cable-like fibrils in the
tough walls that enclose plants
It is a major component of
wood
It is also known as dietary fiber
36
Cellulose
SUGARS
37
Dietary Cellulose
Most animals cannot derive nutrition
from fiber
They have
bacteria in
their digestive
tracts that can
break down
cellulose
38
Carbohydrates
Polysaccharide: many sugar units
Examples: starch (bread, potatoes)
glycogen (beef muscle)
cellulose (lettuce, corn)
glucose
glucose
glucose
glucose
cellulose
glucose
glucose
glucose
glucose
39
Sugars in Water
Simple sugars and double sugars dissolve
WATER
readily in water
MOLECULE
They are
hydrophilic,
or “waterloving”
SUGAR
MOLECULE
40
Lipids
Lipids are hydrophobic –”water fearing”
Do NOT mix with water
Includes
fats,
waxes,
steroids,
& oils
FAT MOLECULE
41
Function of Lipids
Fats store energy, help to insulate the
body, and cushion and protect organs
42
Types of Fatty Acids
Unsaturated fatty acids have less than
the maximum number of hydrogens
bonded to the carbons (a double bond
between carbons)
Saturated fatty acids have the
maximum number of hydrogens bonded
to the carbons (all single bonds
between carbons)
43
Types of Fatty Acids
Single
Bonds in
Carbon
chain
Double bond in carbon chain
44
Triglyceride
Monomer of lipids
Composed of
Glycerol & 3
fatty acid chains
Glycerol forms
the “backbone”
of the fat
Organic Alcohol
45
Triglyceride
Glycerol
Fatty Acid Chains
46
47
Fats in Organisms
Most animal fats have a high proportion
of saturated fatty acids & exist as
solids at room temperature (butter,
margarine, shortening)
48
Fats in Organisms
Most plant oils tend to be low in
saturated fatty acids & exist as
liquids at room temperature (oils)
49
Fats
Dietary fat consists largely of the
molecule triglyceride composed of
glycerol and three fatty acid chains
Fatty Acid Chain
Glycerol
Dehydration links the fatty acids to Glycerol
50
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52
53
Steroids
The carbon skeleton
of steroids is bent
to form 4 fused
rings
Cholesterol is
the “base
steroid” from
which your body
produces other
steroids
Cholesterol
Estrogen
Testosterone
Estrogen & testosterone are also steroids
54
Synthetic Anabolic Steroids
They are variants
of testosterone
Some athletes use
them to build up
their muscles quickly
They can pose
serious health risks
55
Lipids
General term for compounds which are not
soluble in water.
Lipids are soluble in hydrophobic solvents.
Remember: “stores the most energy”
Examples:
1. Fats
2. Phospholipids
3. Oils
4. Waxes
5. Steroid hormones
6. Triglycerides
56
Proteins
Proteins are polymers made of
monomers called amino acids
All proteins are made of 20 different
amino acids linked in different orders
Proteins are used to build cells, act
as hormones & enzymes, and do much
of the work in a cell
57
Four Types of Proteins
Storage
Structural
Contractile
Transport
58
Proteins (Polypeptides)
Amino acids (20 different kinds of aa) bonded
together by peptide bonds (polypeptides).
Six functions of proteins:
1. Storage:
albumin (egg white)
2. Transport:
hemoglobin
3. Regulatory:
hormones
4. Movement:muscles
5. Structural:
membranes, hair, nails
6. Enzymes:
cellular reactions
59
20 Amino Acid Monomers
60
Structure of Amino Acids
Amino acids have a
central carbon with
4 things boded to
it:
Amino
group
Carboxyl
group
R group
Amino group -NH3
Carboxyl group -COOH
Hydrogen
Side group
-H
-R
Side
groups
Serine-hydrophillic
Leucine -hydrophobic
61
Linking Amino Acids
Carboxyl
Cells link amino
acids together to Amino
Side
make proteins
The process is
called dehydration
synthesis
Peptide bonds
form to hold the
amino acids
together
Group
Dehydration
Synthesis
Peptide Bond
62
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Proteins (Polypeptides)
Four levels of protein structure:
A. Primary Structure
B. Secondary Structure
C. Tertiary Structure
D. Quaternary Structure
64
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Primary Protein Structure
The primary
structure is
the specific
sequence of
amino acids in
a protein
Amino Acid
66
Protein Structures
Secondary protein structures occur
when protein chains coil or fold
When protein chains called polypeptides
join together, the tertiary structure
forms
In the watery environment of a cell,
proteins become globular in their
quaternary structure
67
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Protein Structures
Hydrogen bond
Pleated sheet
Polypeptide
(single subunit)
Amino acid
(a) Primary structure
Hydrogen bond
Alpha helix
(b) Secondary
structure
(c) Tertiary
structure
(d) Quaternary structure
71
Denaturating Proteins
Changes in temperature & pH can
denature (unfold) a protein so it no
longer works
Cooking denatures
protein in eggs
Milk protein separates into
curds & whey when it
denatures
72
73
Changing Amino Acid Sequence
Substitution of one amino acid for
another in hemoglobin causes sickle-cell
disease
1
2
(b) Sickled red blood cell
6
7. . . 146
4
5
Normal hemoglobin
(a) Normal red blood cell
1
3
2
3
6
7. . . 146
4
5
Sickle-cell hemoglobin
74
Proteins as Enzymes
Many proteins act as biological catalysts
or enzymes
Thousands of different enzymes exist
in the body
Enzymes control the rate of chemical
reactions by weakening bonds, thus
lowering the amount of activation
energy needed for the reaction
75
Enzymes
Enzymes are globular proteins.
Their folded conformation
creates an area known as the
active site.
The nature and arrangement of
amino acids in the active site
make it specific for only one
type of substrate.
76
Enzyme + Substrate = Product
77
How the Enzyme Works
Enzymes are reusable!!!
78
Enzymes
Free
Energy
Without Enzyme
With Enzyme
Free energy of activation
Reactants
Products
Progress of the reaction
79
80
Nucleic Acids
Store hereditary information
Contain information for making all
the body’s proteins
Two types exist --- DNA &
RNA
81
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Nucleic Acids
Nitrogenous base
(A,G,C, or T)
Nucleic
acids are
polymers of
nucleotides
Phosphate
group
Thymine (T)
Sugar
(deoxyribose)
Phosphate
Base
Sugar
Nucleotide
85
Bases
Each DNA
nucleotide has one
of the following
bases:
–Adenine (A)
Thymine (T)
Cytosine (C)
–Guanine (G)
–Thymine (T)
–Cytosine (C)
Adenine (A)
Guanine (G)
86
Nucleotide Monomers
Form long chains
called DNA
Backbone
Nucleotide
Nucleotides are
joined by sugars
& phosphates on
the side
Bases
DNA strand
87
DNA
Two strands of
DNA join
together to form
a double helix
Base
pair
Double helix
88
DNA5 - double helix
O
3
3
O
P
5
O
C
G
1
P
5
3
2
4
4
2
3
1
P
T
5
A
P
3
O
O
P
5
O
3
5
P
89
RNA – Ribonucleic Acid
Nitrogenous base
(A,G,C, or U)
Ribose sugar
has an extra
–OH or
hydroxyl
group
It has the
base uracil (U)
instead of
thymine (T)
Uracil
Phosphate
group
Sugar (ribose)
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Websites to Explore
http://www2.nl.edu/jste/biochem.htm
Macromolecules of Life (animations)
http://faculty.ccbcmd.edu/biotutorials/energy/adpan.
html
Hydrolysis of ATP molecule
http://www.okc.cc.ok.us/biologylabs/index.htm
OKC Biology Labs Online: Organic Molecules
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Summary of Key Concepts
93
Nucleic Acids
94
Macromolecules
95
Macromolecules
96
http://www.bbc.co.uk/education/asguru/biology/02biologicalmolecules/index.shtml
End
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