Chapter 11.1-11.3 1 11-1 Work of Gregor Mendel 11-2 Probability & Punnett Squares Introduction to Genetics 11-3 Exploring Mendelian Genetics 11-3 Exceptions to Mendelian Genetics 2 Genetics Vocabulary Genetics – the study of passing on of genes from parent to offspring Gene = part of DNA coding for trait Trait = Characteristic Seed shape, seed color, plant height Allele = alternate forms of a gene Trait/ Gene Seed Shape Seed Color Plant Height Allele Round Wrinkled Yellow Green Tall Short Eye color: blue, green, brown, hazel, etc. 3 Gregor Mendel & Pea Plants 7 traits each with 2 contrasting alleles seed shape, seed color, plant height, pod color, pod shape, seed coat color, flower position Self-pollination (also known as true-breeding) vs. Cross-pollination (also known as selective breeding) 4 Mendel’s 1st experiment Mendel controlled the fertilization of his pea plants by removing the male parts, or stamens. He then fertilized the female part, or pistil, with pollen from a different pea plant. 5 Mendel’s 1st Conclusion Law of Dominance Dominant allele v. Recessive allele ○ Capital letter = dominant (ex: tall= T) ○ Lowercase letter = recessive (ex: small= t) Each individual has 2 alleles (homologous) ○ TT =Tall ○ Tt = Tall ○ tt = small Genotype = gene combination (represented by alphabet letters) Phenotype = Physical appearance (represented by words) 6 Mendel’s 2nd Experiment F1 generation x F1 generation Tall (____) x Tall (____) 2nd Experiment Results 7 Mendel’s 2nd Conclusion Law of segregation Alleles separate during gamete formation (meiosis) ○ Homologous chromosomes separate!! (phase?) Review Tall (____) x Tall (____) Phenotype: ○ 3 tall & 1 short 8 Genetic Terminology Genotype: Gene make-up Alleles inherited Homozygous Dominant Ex: Height: TT, Tt, tt Phenotype: Physical appearance ○ Ex: Height ○ Genotype= TT ○ Phenotype= Tall Homozygous Recessive Ex: Height: ○ TT= Tall ○ Tt= Tall ○ tt= Short Homozygous = Purebred Alleles in pair alike ○ Ex: Height ○ Genotype= tt ○ Phenotype= small Heterozygous = Hybrid Alleles in pair different Ex: Height ○ Genotype= Tt ○ Phenotype=Tall 9 Predicting Monohybrid Crosses: Height Punnett Square = diagram to predict offspring Gametes t t t t Larger # offspring closer to expected Monohybrid cross = cross of one trait Homozygous Dominant x Homozygous Recessive T T Step 1: determine parents ○ Height TT x tt Step 2: Put parents on punnett square Step 3: Predict Step 4: Genotypic & Phenotypic ratios T Tt Tt ○ Genotypic- 0 TT:4 Tt: 0 tt ○ Phenotypic- 4 Tall: 0 Short T Tt Tt 10 Punnett Monohybrid Cross: Height Homozygous Dominant x Heterozygous Step 1: ID parents Step 2: add parents to Punnett Step 3: Predict Step 4: Genotypic & Phenotypic ratios ○ Genotypic ratio = ____________ ○ Phenotypic ratio = ____________ Homozygous recessive x Heterozygous Genotypic ratio = _______________ Phenotypic ratio = ______________ 11 Punnett Monohybrid Cross • Heterozygous x Heterozygous Genotypic ratio = ____________ Phenotypic ratio = ____________ • Testcross – Determine unknown genotype of phenotypic dominant trait – Unknown Dominant x recessive Ratios OR Ratios 12 Punnett Dihybrid Crosses Cross 2 pairs of contrasting traits Ex: seed texture & seed color at same time Hom. Round &Yellow x Hom. Wrinkled & Green Step 1: determine parents ○ RRYY x rryy Step 2: determine possible gametes (* independent assortment) ○ RRYY RY, RY, RY, RY ○ rryy ry, ry, ry, ry Step 3: Set-up Punnett Square Step 4: Predict Step 5: Phenotypic ratios 13 Dihybrid Cross Punnett Square ry Phenotypic Ratios RY RY RY RY Round Yellow =16 Round Green = 0 RrYy RrYy RrYy RrYy Wrinkled Yellow=0 Wrinkled Green=0 RrYy RrYy RrYy RrYy Ratio 16:0:0:0 ry RrYy RrYy RrYy RrYy ry RrYy RrYy RrYy RrYy ry 14 Dihybrid Cross Heterozygous x Heterozygous Phenotypic Ratio: Round Yellow= Round Green= Wrinkled Yellow= Wrinkled Green= Ratio: WHY & HOW? 15 Mendel’s 3rd Conclusion Law of independent assortment Alleles on separate chromosomes separate independently of each other during meiosis AKA homologous chromosomes separate independently of each other (phase?) Accounts for genetic variation!! Review of possible gametes in dihybrid cross RRYY RY, RY, RY, RY rryy ry, ry, ry, ry Due to Law of Independent assortment 16 Mendel Summary Traits determined by genes & are passed on 2. Most sexually reproducing adults have 2 copies of each gene (AKA 2 alleles) one from each parent 3. Law of Dominance vs Recessive 4. Law of Segregation 5. Law of Independent Assortment 1. 17 Artificial Selection Selective Breeding – known as artificial selection, people select the traits they want to have passed on. Inbreeding – can cause genetic defects because the DNA is too similar to each other. Hybridization – A mix of two different traits 18