OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Bovine Spongiform Encephalopathy
Address of laboratory
3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856 JAPAN
Tel.:
+81-29-939-7840
Fax:
+81-29-939-8332
e-mail address:
tyoko@affrc.go.jp
website:
http://www.naro.affrc.go.jp/org/niah/
Name (including Title) of Head of
Laboratory (Responsible Official):
Dr. Takafumi Hamaoka
Name (including Title and Position) of OIE
Reference Expert:
Dr. Takashi Yokoyama
Date of submission to the OIE
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January
through December of the past year (2012), unless otherwise stated, and must be submitted by the end of
January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE
should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference
Laboratories, available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute
with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference
Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Bovine Spongiform Encephalopathy » – « Takashi Yokoyama » – « Japan »
Test recommended by the OIE
2.
Total number of test performed last year
Direct diagnostic tests
Nationally
Internationally
Confirmatory test for BSE (WB, IHC)
0
0
Surveillance of scrapie (ELISA, WB, IHC)
154
0
Surveillance of CWD (WB)
21
0
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
Type of reagent
available
Control BSE positive
brain samples
4.
Related
diagnostic
test
WB,
ELISA
No
Produced/
stored
Amount supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
Name of
recipient OIE
Member
Countries and
of institutions
Produced
３(positive)
3g (gｘ3)
QIA, South Korea
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
5.
No
Did your laboratory produce vaccines?
Yes
6.
Did your laboratory supply vaccines to OIE Member Countries?
Yes
7.
No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
Name of the new test or diagnostic
method or vaccine developed
PMCA (Protein misfolding cyclic
amplification)
2
No
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
No
No
Description and References (Publication, website, etc.)
Okada H et al., (2012). rion in saliva of bovine spongiform
encephalopathy-infected cattle. Emerg Infect Dis. 18:
2091-2.
Annual reports of OIE Reference Centres, 2012
OIE RL for « Bovine Spongiform Encephalopathy » – « Takashi Yokoyama » – « Japan »
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
10.
No
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
No
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was
provided
National Institute of Animal Health,
Taiwan
BSE surveillance strategy
In loco
Science Academia, Taiwan
Inactivation of prion
In loco
QIA, South Korea
Diagnosis of TSE
In loco
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
No
Title of the study
Duration
Purpose of the study
Establishment of
CWD-prion
susceptible cell lines
1 year
To establish the in
vitro assay system for
CWD prions
12.
Partners
(Institutions)
OIE Member Countries
involved other than
your country
QIA, Anayng
South Korea
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
No
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 14
b)
International conferences: .............................................. 13
c)
National conferences: ...................................................... 0
d)
Other: ............................................................................... 0
a) Published peer-reviewed journals
1．Okada H, Iwamaru Y, Fukuda S, Yokoyama T, Mohri S. (2012) Detection of disease-associated prion
Annual reports of OIE Reference Laboratories, 2012
3
OIE RL for « Bovine Spongiform Encephalopathy » – « Takashi Yokoyama » – « Japan »
protein in the optic nerve and the adrenal gland of cattle with bovine spongiform encephalopathy by
using highly sensitive immunolabeling procedures. J Histochem Cytochem. 60: 290-300.
2. Fukuda S, Onoe S, Nikaido S, Fujii K, Kageyama S, Iwamaru Y, Imamura M, Masujin K, Matsuura Y,
Shimizu Y, Kasai K, Yoshioka M, Murayama Y, Mohri S, Yokoyama T, Okada H. (2012) Neuroanatomical
distribution of disease-associated prion protein in experimental bovine spongiform encephalopathy in
cattle after intracerebral inoculation. Jpn J Infect Dis. 65: 37-44.
3. Kasai K, Hirata A, Ohyama T, Nokihara K, Yokoyama T, Mohri S. (2012) Novel assay with fluorescencelabelled PrP peptides for differentiating L-type atypical and classical BSEs, and scrapie. FEBS Lett. 586:
325-9.
4. Masujin K, Miwa R, Okada H, Mohri S, Yokoyama T. (2012) Comparative analysis of Japanese and
foreign L-type BSE prions. Prion 6: 89-93.
5. Tang Y, Gielbert A, Jacobs JG, Baron T, Andreoletti O, Yokoyama T, Langeveld JP, Sauer MJ. (2012) All
major prion types recognised by a multiplex immunofluorometric assay for disease screening and
confirmation in sheep. J Immunol Methods. 380: 30-9.
6. Murayama Y, Imamura M, Masujin K, Shimozaki N, Yoshioka M, Mohri S, Yokoyama T. (2012)
Ultrasensitive detection of scrapie prion protein derived from ARQ and AHQ homozygote sheep by
interspecies in vitro amplification. Microbiol Immunol. 56:541-7.
7. Kim HJ, Tark DS, Lee YH, Kim MJ, Lee WY, Cho IS, Sohn HJ, Yokoyama T. (2012) Establishment of a
Cell Line Persistently Infected with Chronic Wasting Disease Prions. J Vet Med Sci. 74: 1377-1380.
8. Dorj G, Okada H, Miyazawa K, Masujin K, Kimura K, Mohri S, Yokoyama T. (2012) Retrospective
Analysis of Sheep Scrapie by Western Blotting with Formalin-Fixed Paraffin-Embedded (FFPE)
Tissues. J Vet Med Sci. 74: 1207-1210.
9. Iwamaru Y, Takenouchi T, Murayama Y, Okada H, Imamura M, Shimizu Y, Hashimoto M, Mohri S,
Yokoyama T, Kitani H. (2012) Anti-prion activity of Brilliant Blue G. PLoS One. 7:e37896.
10. Takenouchi T, Iwamaru Y, Imamura M, Fukuhara S, Sugama S, Sato M, Mochizuki N, Hashimoto M,
Yokoyama T, Mohri S, Kitani H. (2012) Cytochalasin D enhances the accumulation of a protease-resistant
form of prion protein in ScN2a cells: involvement of PI3 kinase/Akt signalling pathway. Cell Biol Int. 36:
1223-31.
11. Okada H, Murayama Y, Shimozaki N, Yoshioka M, Masujin K, Imamura M, Iwamaru Y, Matsuura Y,
Miyazawa K, Fukuda S, Yokoyama T, Mohri S. (2012) Prion in saliva of bovine spongiform
encephalopathy-infected cattle. Emerg Infect Dis. 18:2091-2.
12. Okada H et al., Properties of L-type bovine spongiform encephalopathy in intraspecies passages. Vet
Pathol 49(5), 819-823 (2012)
13. Okada H et al., Immunohistochemical detection of disease-associated prion protein in the peripheral
nervous system in experimental H-type bovine spongiform encephalopathy. Vet Pathol,
doi:10.1177/0300985812471541 (in press)
14. K. Hasegawa, S. Mohri, T. Yokoyama. Comparison of the local structural stabilities of mammalian
prion protein (PrP) by fragment molecular orbital calculations. Prion (in press).
b) International Conference
1. K. Masujin, R. Miwa, H. Okada, S. Mohri, T. Yokoyama. Comparative analysis of Japanese and other
countries L-type BSE prions. Prion 2012, Amstrdam, May 10-12, 2012.
2. J. Jacobs, J. Langeveld, T. Yokoyama, O. Andreoletti, J. Hope, A. Bossers, L. van Keulen, T. Baron.
Ovine field CH1641 like scrapie cases do differ from classical scrapie and BSE–Western blotting studies
on PrPres. Prion 2012, Amstrdam, May 10-12, 2012.
3. T. Yokoyama, G. Dorj, H. Okada, K. Miyazawa, K. Masujin, S. Mohri. Western blotting detection of
abnormal prion protein (PrPSc) from formalin-fixed paraffin-embedded (FFPE) tissues–Immunoreactivity
of mab discriminate sheep scrapie types. Prion 2012, Amstrdam, May 10-12, 2012
4. M. Kuroda, J. Kono, Y. Sato, T. Ohashi, T. Yokoyama, Y. Ushiki, T. Onodera, M. Yukawa.
Application of micro-ELISA for detection of prion protein from small volume of sample. Asian
Pacific Prion Symposium 2012 (APPS 2012). Yokohama, July 29-30, 2012
5. K. Kasai, A. Hirata, T. Ohyama, K. Nokihara, T. Yokoyama, S. Mohri. Novel assay with
fluorescence-labelled PrP peptides for differentiating L-type atypical and classical BSEs, and
scrapie. Asian Pacific Prion Symposium 2012 (APPS 2012). Yokohama, July 29-30, 2012
6. G. Dorj, H. Okada, K. Miyazawa, K. Masujin, S. Mohri, T. Yokoyama. Retrospective analysis of
sheep scrapie by western blotting with formalin-fixed paraffin-embedded (FFPE) tissues. Asian
Pacific Prion Symposium 2012 (APPS 2012). Yokohama, July 29-30, 2012.
7. Y. Nagasawa, Y. Takahashi, T. Hondo, H. Watanabe, S. Terada, C. Saito, S. Someya, K.
Watanabe, S. Ohwada, H. Kitazawa, M. Imamura, T. Yokoyama, S. Sakaguchi, N. Nishida, S.
4
Annual reports of OIE Reference Centres, 2012
OIE RL for « Bovine Spongiform Encephalopathy » – « Takashi Yokoyama » – « Japan »
Mohri , H. Aso. Prion protein binding proteins of bovine intestine M cell. Asian Pacific Prion
Symposium 2012 (APPS 2012). Yokohama, July 29-30, 2012.
8. S. Fukuda, T. Fujii, S. Kageyama, H. Okada, T. Yokoyama, Y. Murayama. Preclinical detection of
PrPSc in eyes of BSE-affected cattle. Asian Pacific Prion Symposium 2012 (APPS 2012). Yokohama, July
29-30, 2012.
9. T. Yokoyama Atypical bovine spongiform encephalopathies. The 16th Annual meeting of Japanese society
for neurovirology. Tokyo, August 30-31, 2012. 1)
10. M. Imamura, N. Kato, M. Yoshioka, H. Okada, Y. Iwamaru, Y. Shimizu, S. Mohri, T.
Yokoyama, Y. Murayama. Proteinase K- and heat-treated insect cell lysate effectively stimulates in
vitro high-fidelity replication of baculovirus-derived recombinant scrapie and BSE prions. Asian
Pacific Prion Symposium 2012 (APPS 2012). Yokohama, July 29-30, 2012.
11. Y. Iwamaru, T. Takenouchi, M. Imamura, S. Mohri, T. Yokoyama, H. Kitani. Prion-mediated
cytotoxicity in a differentiated neurosphere culture. Asian Pacific Prion Symposium 2012 (APPS 2012).
Yokohama, July 29-30, 2012.
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
No
a)
Technical visits: ................................................................ 3
b)
Seminars: ......................................................................... 0
c)
Hands-on training courses: .............................................. 0
d)
Internships (>1 month): ................................................... 1
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s)
provided with training
No. participants from the
corresponding country
a
China
3
d
Indonesia
1
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
17.
No
Is your laboratory accredited by an international accreditation body?
Yes
No
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
19.
No
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
Annual reports of OIE Reference Laboratories, 2012
No
5
OIE RL for « Bovine Spongiform Encephalopathy » – « Takashi Yokoyama » – « Japan »
National/
International
Title of event
Co-organiser
Date
(mm/yy)
Location
No.
Participants
International
Asia pacific prion
symposium 2012
APSPR (Asia
pacific society for
prion research)
July 2012
Yokohama,
Japan
100
20.
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
No
Title of event
Date
(mm/yy)
Location
Role (speaker,
presenting poster, short
communications)
Title of the work presented
Prion 2012
05/12
Prion 2012,
Amstrdam,
Speaker
Western blotting detection of
abnormal prion protein (PrPSc)
from formalin-fixed paraffinembedded (FFPE) tissues.
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
No
Title of the project or contact
Scope
Name(s) of relevant OIE
Reference Laboratories
Workshop on CWD
CWD surveillance of wild deer
OIE RL for CWD, South Korea
Discussion for the
establishment of mutual
collaboration
CWD surveillance of wild deer
OIE RL for CWD, Canada
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
6
No
No
Annual reports of OIE Reference Centres, 2012