) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
University of Baghdad
College Name
College of science
Department
Biology
Full Name as written
in Passport
Hala . M. Radef
e-mail
Not found
Career
Assistant Lecturer
Lecturer
Master
Thesis Title
Year
Assistant Professor
Professor
PhD
Compare study between spiral and cocci shape of Helicobacter pylori isolated
from duodenal ulcer .
2006
SUMMARY
Abstract
The study aimed to isolate the Helicobacter pylori bacteria from
patients who have duodenum ulcer and study the phenomenon of cell
transformation from spiral shapes to cocci shapes and the influencing
factors. The results showed that the component of the agricultural
environment, light, and oxygen are the most important factors that help
in the process of transformation from the spiral shapes to cocci bacterial
shapes of H. pylori during the incubation and the storage period. It had
been noticed that when using Brain-Hart infusion agar and Blood-Base
agar with the addition of decomposed human blood by a percentage of
(5%) with growth supplements, we get cocci shapes with a percentage
less than that when we use the same agricultural media without adding
the blood and the growth supplements. To maintain the activity of the
H. pylori and its spiral shape for a longer period of time which can go up
to (20-30) days when stored at 4 Co , we must not exposure the
agricultural media to light and oxygen with a percentage higher than 6%
and also thepH must be (6.9-8).
For the first time, it had been recorded that some cocci shapes can
transform to spiral shapes when using Brain-Hart infusion agar, MullerHinton agar, Brucella agar, Milk agar, Choclate agar, Maconcky agar, and
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
Trypton Soy Broth, if we add to some of the used media (5%) of
decomposed human blood and growth supplements.
While, the
previous studies mentioned that it is not possible for the cocci shapes to
transform to spiral shapes when using cultural media. Also, it had been
recorded for the first time that some of the cocci shapes can transform
to spiral shapes when using mice and Guinea-Pigs, in a better way than
using the solid and liquid cultural media taking into consideration giving
the animal a dosage of vaccine of size (0.1) ml. Tissue damage had been
noted when studying the tissue sections for stomach, while previous
studies mentioned that it is not possible for the cocci shapes to
transform to spiral shapes when using laboratory animals.
The results show that the chemical content of the cocci shapes in the
H.pylori bacteria is different from the chemical content of the spiral
shapes of the same type of bacteria after treating it by concentrated
sulfuric acid and potassium hydroxide, which reflect that changes had
took place in the cell wall during the process of transformation from
spiral shapes to cocci shapes.
The results also showed that the cocci shapes are less density from
the spiral shapes due to shortage of Peptidoglycan and the vanishing of
the cellular components and the loss of these cellular components due
to the changes that took place in the cell wall and the damage of the
Cytoplasmic membrane.
The study showed that the contents of the cocci shapes of the H.pylori
from the Deoxyribo Nucleic Acid (DNA) and from the RiboNucleic Acid
(RNA) is less than that of the spiral shapes of the same bacteria.
When the Lysozyme and EDTA had been studied separately and also
the Lysozyme and the DETA together in the spiral shapes of the H. pylori
bacteria, the effect was much more when compared with the cocci
shapes of the same bacteria, and that is due to the shortage of
Peptidoglycan in the walls of the cocci shapes or due to the loss of
Peptidoglycan positions sensitive to the Lysozyme.
It had been noted that the ionic detergents such as the SDS and the
non-ionic detergents such as Triton X-100 are causing the analysis of
the H. pylori isolation in both the cocci and spiral shapes, but the low
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
concentrations of SDS is more efficient than the high concentration of
the Triton X-100 in the analysis of the bacteria in both of its cocci and
spiral shapes.
The spiral shapes and the cocci shapes of the H. pylori bacteria and the
shapes that appears during the process of transformation from spiral
shapes to cocci shapes had been observed when using the Transmission
Electronic Microscope (TEM) , also different cocci shapes had been
observed that have one and two flagellum.
It had been recorded for the first time that the cocci shapes have more
than one flagellum and that was during the photography of the
isolations by the TEM, while previous studies mentioned that the cocci
shapes have one or two flagellum only. The fine composition study
using electronic microscope showed that many shapes of the bacteria
appears ( rods, curved rods, and the spiral ) in addition to noticing the
round end of the bacteria and the homogenous distribution of the
Cytoplasmic and Chromatic contents inside the bacteria cell and the
smooth surface of the bacteria, and also the flagellum that distinguish
this bacteria had been observed that are distinguished by being
sheathed
and
have
( thickness ) in the end of the flagellum which looks like cudgel.
inflation