What do I and my classmates already know re: cells? 11/2/15 Animal cells are different from plant cells Cells are alive, so they can die Many different types of cells All eukaryotic cells have a nucleus Animal cells are not connected to each other like plant cells Cells are basic building block of life Plant cells are a box Animal cells are circular; any shape but a box Can only see cells through a microscope Need cells in order to be living Cells can reproduce Plant cells have a cell wall Cells make tissues, tissues make organs Plant cells are tougher than animal cells…more rigid/studier Come in 1000’s of shapes & sizes All life is made of cells 500 quadrillion chem. rxns in a cell every second Over 100 trillion cells in human body…more cells in one human that there are total humans on Earth 10,000 cells fit on the head of pin What is the Cell Theory and Hx of microbiology? 11/4/15 CELL THOERY Cell Theory= explanation of how cells & life are related Cell Theory states: all living things are composed of cells, cells are the basic unit of structure & fxn-smallest unit of life, cells come from other cells Schleiden, Schwann, Virchow made the Cell Theory in mid-1800’s Hx OF MICROBIOLOGY microbiology= study of microscopic (teeny tiny) living things… “micro”=teeny tiny… “bio”= life… “ology”=study of 1590 invention of microscope made seeing cells possible…Zacharias Janssen Schleiden was a botanist Robert Hooke coined the term “cell” in 1660’s while looking at cork Mid-1800’s Robert Brown discovered nucleus Leeuwenhoek was 1st to see unicellular organisms (bacteria)…looking @ spit & teeth scrapings What are the ingredients of cells? 11/13/15 Cells are made of… -DNA & RNA (nucleic acids)=1.5 % wgt -lipids (fats)=1% wgt -proteins=5% wgt -carbohydrates (sugars)=2.5% wgt -H2O=90% of wgt What is the basic structure of eukaryotic cells? 11/16/15 organelle= “organ” of the cell; parts & pieces inside a cell that do the cell’s body fxns. (taped in chart of 12 cell organelles) plants cells are boxy shaped so they can stack for height…animal cells are anything but boxy shaped cells only have the organelles they need to do their job --EX: plant root cells don’t have chloroplasts b/c they don’t need them (tape in) How does the structure of plant and animal cells compare? 12/4/15 plant cell only cell wall chloroplasts (1: organelle difference) boxy shaped (2: shape) 1 BIG vacuole (3: vacuole difference) BOTH ribosomes vacuole nucleus ER cell membrane cytoplasm mitochondria Golgi body animal cell only lysosomes cytoskeleton every shape but a box many small vacuoles How does the cell membrane work? 1/4/16 cell membrane is selectively permeable= tiny holes to control what comes in and out of the cell; small molecules pass through, lrg ones don’t diffusion= molecules moving across the cell membrane from high to low concentration until reach equilibrium=even/balanced molecules move b/c they bump off e@ other osmosis= diffusion of H2O many cellular fxns depend on osmosis (diagram of diffusion/osmosis) isotonic= even amt of H2O inside and outside of the cell…ideal state of equilibrium/homeostasis hypotonic= H2O rushes into the cell b/c not a lot of it in the cell…animal cells explode but plant cells swell to rigidity (high H2O concentration so swollen, does NOT need H2O) hypertonic=H2O rushes out of the cell b/c not a lot of it outside of the cell…plants wilt, animals get thirsty/dehydrated…(low H2O concentration so needs H2O) What is the difference between passive and active transport across the cell membrane? 1/12/16 ACTIVE TRANSPORT PASSIVE TRANSPORT -cell uses energy to get stuff across the cell membrane -cell DOES NOT use energy to get stuff across membrane -uses transport proteins as a “bridge/doorway” to get big molecules across the membrane -engulfing is another way cell’s get big stuff inside -like going uphill Both ways things pass through the cell membrane -diffusion & osmosis -like going downhill What is the structure and fxn of DNA found in the nucleus? 1/13/16 (tape-in highlighted for information) DNA codes for proteins that determine what makes me look like me=phenotype….makes a pepper tree, a pepper tree, etc. Shape/structure discovered by Watson & Crick in 1953 The color of all DNA is white What is photosynthesis? 1/22/16 Photosynthesis= the process of an plant autotrophic cell getting energy from sunlight to make food…converting radiant energy into chemical energy that organisms can use…use sunlight energy to convert CO2 and H2O into O2 and sugar Ingredients for photosynthesis: What is required and how does the plant get it? 1) sunlight (photons) captured in the chloroplasts by chlorophyll= green chemical “solar panel” 2) CO2 (carbon dioxide) from the atmosphere enters through leaf stomata= small pores on the underside of leaf and into the cell by diffusion 3) H2O (water) from the soil is sucked up by the roots through osmosis The process of photosynthesis: a WICKED chemical rxn by the chloroplast using the sun’s energy to rip apart H2O & CO2 molecules in to C, H, O atoms to make Products of photosynthesis: 1) Oxygen (O2)—released into the air through the stomata 2) Sugar (glucose: C6H12O6)—as energy for life’s functions or stores it as starch (plant fat) Photosynthesis equation: 6CO2+6H20+sunlight photons yields C6H12O6+6O2 “six carbon dioxide molecules plus six water molecules + sunlight photons yields one glucose molecule plus six oxygen molecules” Why do the leaves of some trees change color in autumn? 2/3/16 Carotenoids= orange/yellow color of leaves…always there! Conifers have waxy coat to protect from freezing, so they DON’T loose leaves…pines! Days get shorter, so trees stop making chlorophyll b/c there is not as much sun as there used to be…no longer able to do effective photosynthesis Deciduous trees= go dormant/ “hibernate” by losing leaves Anthocyanins=red pigment in leaves made @ end of summer What is cellular respiration? 2/4/16 Cellular respiration= cells breaking down food to release energy…in the mitochondria ALL living organisms do CR Ingredients of CR: 1) Glucose/sugar (C6H12O6)…animals eat food to get it; plants do photosynthesis to get it 2) Oxygen…from the atmosphere; animals inhale, plants through the stomata Process of CR: a WICKED chemical rxn by mitochondria…glucose is ripped apart to release energy Products of CR: 1) Carbon dioxide (CO2)…released through plant stomata; animals exhale 2) Water (H2O)…released during exhalation in animals & through stomata in plants 3) ENERGY in the form of ATP…used for living Cellular Respiration equation: C6H12O6+6O2 6H2O+6CO2+energy (ATP) One glucose molecule plus six oxygen molecules yields six water molecule plus 6 carbon dioxide molecule plus energy as ATP (38). How are photosynthesis and cellular respiration related? 2/16/16 Opposite reactions! Products on one are the ingredients of the other Need e@ other to exist: w/o photosynthesis there is no CR and vice versa Work together to keep O2 and CO2 in balance in the atmo. What is fermentation? 2/23/16 fermentation= cells releasing energy w/o O2…less energy released than CRNOT as efficient as CR 2 types of fermentation: 1) alcoholic fermentation= done by yeast…puts alcohol & bubbles in beer & wine and makes bread rise C6H12O6+zymase 2 C2H5OH+2 CO2 (alcohol) 2) lactic acid fermentation= done by some bacteria & muscle cells…makes muscles sore & weak after heavy exercise (muscles) and makes yogurt & sauerkraut (bacteria) C6H12O6 2 CH3CHOHCOOH (lactic acid) What is the cell cycle…AKA mitosis? 2/24/16 cell cycle= sequence of growing & dividing in cells 3 stages to the cell cycle: 1) Interphase: cell grows, replicates DNA & prepares to divide into two “daughter cells” --DNA replicates by untwisting, “unzipping” and “zipping” in a new ½ to create two, identical copies --about 90% of the total time (diagram) 2) Mitosis= “make two, identical nuclei” out of one nucleus in 4 stages/phases *Prophase= DNA “packs together” in condensed chromosomes *metaphase= DNA lines up in the “middle” & nuclear membrane dissolves *anaphase= DNA pairs pulled “apart/away from e@ other” by spindle fibers (thread thingies) *telophase= “two, identical, nuclei” in ONE, stretched out cell --about 8% of the total time 3) Cytokinesis= cytoplasm divides into two new, identical cells…cytoplasm’s final split --“complete split”, “cut in ½” --cell membrane pinches in to cut in ½ in animal cells --cell plate forms in plant cells to cut in ½ --about 2% of the total time What is differentiation? 3/7/16 Fxn #4 Cellular differentiation= the process of cells changing structure & fxn to become specialized in an organism… “has found a career and will stay there until death” Happens when sections of DNA are turned OFF Stem cell= undifferentiated cell…like Play Doh that can be shaped into anything… “doesn’t know what it wants to be when it grows up b/c can be anything the organism needs” Regeneration= ability to grow new body parts b/c of stem cells @ to point of injury EX: lizard tails, starfish arms, pothos/creeping charlie roots