UNIVERSITY OF FLORIDA
RADIATION SAFETY
SHORT COURSE
Radioisotope Laboratory Techniques
Day 2
Environmental Health & Safety
Radiation Control & Radiological
Services
Our Goals
•To provide you with the information to work
smarter and safer!
•To fulfill the requirements of the University of
Florida’s Radioactive Materials license.
Lab Safety Checklist
• Complete Radiation Safety Inspection
• Quarterly
• Swipe survey
• Lists any deficiencies
Lab Safety Checklist
1. Caution Signs
a.
b.
c.
d.
e.
f.
Doors
Hoods
Hardware
Work Areas
Refrigerators / Freezers
Waste Areas
Lab Safety Checklist
1. Caution Signs
a.
b.
c.
d.
e.
f.
Doors
Hoods
Hardware
Work Areas
Refrigerators / Freezers
Waste Areas
Lab Safety Checklist
1. Caution Signs
a.
b.
c.
d.
e.
f.
Doors
Hoods
Hardware
Work Areas
Refrigerators / Freezers
Waste Areas
Lab Safety Checklist
1. Caution Signs
a.
b.
c.
d.
e.
f.
Doors
Hoods
Hardware
Work Areas
Refrigerators / Freezers
Waste Areas
Lab Safety Checklist
2. Required Postings
a.
b.
c.
d.
Emergency Spill Procedures
Notice to Employees
Emergency Notification
Laboratory Safety Rules
Lab Safety Checklist
2. Required Postings
a.
b.
c.
d.
Emergency Spill Procedures
Notice to Employees
Emergency Notification
Laboratory Safety Rules
Lab Safety Checklist
2. Required Postings
a.
b.
c.
d.
Emergency Spill Procedures
Notice to Employees
Emergency Notification
Laboratory Safety Rules
Lab Safety Checklist
2. Required Postings
a.
b.
c.
d.
Emergency Spill Procedures
Notice to Employees
Emergency Notification
Laboratory Safety Rules
Lab Safety Checklist
3. Survey Instruments
a.
b.
c.
d.
Battery Check
Calibrated within the last 9 months?
Proper meter for isotopes in use?
Is LSC information the same as last inspection?
Lab Safety Checklist
4. Waste Areas
a.
b.
c.
d.
Properly Shielded?
Properly Segregated?
Stored properly?
Are appropriate catchtrays under liquids?
Lab Safety Checklist
5. Protective Clothing
a. Lab coat?
b. Appropriate gloves?
c. Eye protection?
UNIVERSITY OF FLORIDA RADIOACTIVE MATERIALS USER
STATEMENT OF TRAINING AND EXPERIENCE
(To be completed by ALL personnel who will be working with radioactive materials at the University of Florida)
Lab Safety Checklist
6. Lab Surveys and Documentation
a. RC-1 Forms current?
b. Radioisotope forms maintained (track
RAM from cradle to grave)?
c. Radioisotope transfer forms
maintained?
d. Adequate survey frequency and
documentation?
e. Spills involving personnel or > 100 mCi?
f. Follow-up surveys, documentation,
and decon of contaminated areas >
100mCi?
g. Waste disposal receipts?
NAME:
DEPARTMENT:
PHONE:
CLASSIFICATION (Faculty, Technician, Student, etc.):
RADIOACTIVE MATAERIAL
TO BE USED:
PRINCIPAL INVESTIGATOR:
RADIATION SAFETY TRAINING
SUBJECT
WHERE TRAINED
DATES AND
DURATION OF
TRAINING
PRECEPTOR/
ON THE JOB
Yes
No
FORMAL
COURSE
Yes
No
A.
Principles and practices
of radiation protection
B.
Radioactivity Measurement,
standardization, monitoring
techniques, and instruments
Yes
No
Yes
No
C.
Mathematics and calculations
basic to use and measurement
of radioactivity
Yes
No
Yes
No
D.
Biological effects of radiation
exposure
Yes
No
Yes
No
materials
Yes
No
Yes
No
Operating and Emergency
procedures
Yes
No
Yes
No
E. Transportation of radioactive
F.
RADIOACTIVE MATERIAL HANDLING EXPERIENCE
RADIONUCLIDE
USED
MAXIMUM
AMOUNT
WHERE EXPERIENCE
WAS GAINED
DATES AND DURATION OF
EXPERIENCE
TYPE OF
USE
Have radiation exposure records been maintained for you at another institution?
Yes
I have read and will abide by the University regulations as set forth in the RADIATION CONTROL GUIDE.
Signature: __________________________________________________
Date:
If additional space is needed, use the back of this sheet. Keep a copy and return original to:
RADIATION CONTROL DEPARTMENT - 212 Nuclear Sciences Center - Box 118340
No
RADIONUCLIDE UTILIZATION FORM
PRINCIPAL INVESTIGATOR: Dr. No
RADIONUCLIDE: P-32
Lab Safety Checklist
CHEMICAL/PHYISICAL FORM: liq/aqueous
INITIAL ACTIVITY RECEIVED (Ci or mCi): 5 mCi
DATE RECEIVED:
Transferred from PI:
Transfer Approval Date:
06/04/08
(USE A SEPARATE FORM FOR EACH SHIPMENT OF EACH RADIONUCLIDE RECEIVED)
6. Lab Surveys and Documentation
a. RC-1 Forms current?
b. Radioisotope forms maintained (track
RAM from cradle to grave)?
c. Radioisotope transfer forms
maintained?
d. Adequate survey frequency and
documentation?
e. Spills involving personnel or > 100 mCi?
f. Follow-up surveys, documentation,
and decon of contaminated areas >
100mCi?
g. Waste disposal receipts?
ROOM NO: DEN-252
Activity
Remaining
Ci-mCi
4.3 mCi
Use
Final Disposal
06/04/18
Activity
Removed
Ci-mCi
0.7 mCi
Gel shift
Liquid waste
Users
Initials
JT
06/11/08
2.0 mCi
2.3 mCi
RNase protection
Solid waste
DD
06/11/08
0.5 mCi
1.8 mCi
Northern Blot Anal.
Liquid waste
DD
06/12/08
1.8 mCi
0
Disposed remain. activity
Solid waste
JT
Date
NOTE: When this particular shipment of radioactive material has been completely utilized, decayed or
disposed, maintain this form in laboratory files for review by State and University Radiation Control
inspectors.
Lab Safety Checklist
6. Lab Surveys and Documentation
a. RC-1 Forms current?
b. Radioisotope forms maintained (track
RAM from cradle to grave)?
c. Radioisotope transfer forms
maintained?
d. Adequate survey frequency and
documentation?
e. Spills involving personnel or > 100 mCi?
f. Follow-up surveys, documentation,
and decon of contaminated areas >
100mCi?
g. Waste disposal receipts?
AFTER
EACH
USE!
Lab Safety Checklist
6. Lab Surveys and Documentation
a. RC-1 Forms current?
b. Radioisotope forms maintained (track
RAM from cradle to grave)?
c. Radioisotope transfer forms
maintained?
d. Adequate survey frequency and
documentation?
e. Spills involving personnel or > 100 mCi?
f. Follow-up surveys, documentation,
and decon of contaminated areas >
100mCi?
g. Waste disposal receipts?
University limits for
removable
contamination:
Beta/Gamma 100 dpm / 100 cm²
Alpha 50 dpm / 100 cm²
RADIATION/CONTAMINATION SURVEY
PRINCIPAL INVESTIGATOR: Dr. Who
When to
document a
survey?
A survey must be documented
within seven days of isotope
use
(Best to schedule one day a
week)
OR
If the lab has isotope storage
only (no use) or waste still on
hand…a documented survey
once a month
SURVEYOR: Jack
ROOM NO:
13
RADIONUCLIDES USED: P-32
SURVEY NO: 10
SURVEY TYPE: WEEKLY
DATE: 05/10/08
RADIATION READINGS:
Yes
X
Caution Signs Posted
Waste Facilities Posted
& Neat
Inventory up to Date
X
Work Areas Clean
X
Survey Meter Calibrated
X
No
N/A
Survey Meter Model & Serial #
Ludlum L-3 SN 5288974
Work Area
Waste Facilities
Storage Areas
X
Survey Meter Operation
Verified with Dedicated
Check Source
X
0.004
0.003
0.004
mR/hr
mR/hr
mR/hr
CONTAMINATION SURVEY RESULTS
Counting Instrument Model & Serial #
Beckman LS-8000 SN 8577571152
DETAILED LABORATORY LAYOUT
Detection Efficiency 75% for Iosotope P-32
1
11
#
5
12
8
9
10
7
3
2
6
4
Swipe
Identification
Net
cpm
Net
dpm*
Post
Decon
dpm
1
Bench
74
99
2
Floor
250
333
54
3
Sink
20
26
4
Door
50
67
5
Floor
5822
7762
88
6
Phone
40
53
7
Cabinet
10
13
8
Fuge
44
59
9
Hot plate
13
17
10
Liq waste jug 55
73
11
Desk
10
13
12
Floor
60
80
*Areas of greater than 100 dpm/swipe must be
resurveyed and documented.
DPM = Gross CPM minus background divided by the
efficiency of the counting instrument for the particular
radioisotope.
Lab Safety Checklist
6. Lab Surveys and Documentation
a. RC-1 Forms current?
b. Radioisotope forms maintained (track
RAM from cradle to grave)?
c. Radioisotope transfer forms
maintained?
d. Adequate survey frequency and
documentation?
e. Spills involving personnel or > 100
mCi?
f. Follow-up surveys, documentation,
and decon of contaminated areas >
100mCi?
g. Waste disposal receipts?
Minor
Spill
< 100 µCi of activity
< 5 mR/hr at one foot
In the Event of a Minor Spill
 Notify everyone in the area
 Prevent the spread of contamination
 Call for help if needed
 Decontaminate
• Only trained staff may handle spill
• Monitor potentially contaminated staff first
(before leaving the area)
• Lab coat and gloves (shoe covers if necessary)
• Start from the outside and work your way in
• Use absorbent paper and detergent as needed
 Survey
• Below 100 dpm / 100 cm²
Major Spills
• Personal contamination or injury
• >100 µCi of activity
• >5 mR/hour at one foot
In the Event of a Major Spill
 Remove individuals from danger
 Call 911 if serious injury
 Limit the spread of contamination &
shield if possible
 Call Radiation Control
 Close, lock and post the room
 Decontaminate under the direction of
Radiation Control
Personal Decontamination:








Remove contaminated clothing
Use sticky tape
Flush with water
Soap and warm water
Mild abrasives, soft brush and water
Detergents
Mild organic acid (citric)
Wear gloves
Lab Safety Checklist
6. Lab Surveys and Documentation
a. RC-1 Forms current?
b. Radioisotope forms maintained (track
RAM from cradle to grave)?
c. Radioisotope transfer forms
maintained?
d. Adequate survey frequency and
documentation?
e. Spills involving personnel or > 100 mCi?
f. Follow-up surveys, documentation,
and decon of contaminated areas >
100mCi?
g. Waste disposal receipts?
Lab Safety Checklist
6. Lab Surveys and Documentation
a. RC-1 Forms current?
b. Radioisotope forms maintained (track
RAM from cradle to grave)?
c. Radioisotope transfer forms
maintained?
d. Adequate survey frequency and
documentation?
e. Spills involving personnel or > 100 mCi?
f. Follow-up surveys, documentation,
and decon of contaminated areas >
100mCi?
g. Waste disposal receipts?
Lab Safety Checklist
7. General Lab Safety
a. Fume hood calibrated every 12
months?
b. Absorbent paper used in designated
work areas (plastic side down)?
c. Food or drinks in work area?
d. Proper shielding for isotopes?
e. Adequate security?
f. Survey of hands and feet after every
use?
Top Priority….. SECURITY!
 Must be locked at
all times when no
one is present
 Includes radioactive
waste
 Challenge everyone
that you do not
know!
Lab Safety Checklist
8. Personnel Monitoring
a. Whole body badge?
b. Extremity badge (P-32 in quantities >
1mCi)?
c. Exposure reports readily available?
d. Thyroid counts required (I-125 > 1mCi in
any 1 month)
e. Urine bioassay required for quantities
of H-3 > 25 mCi in any 1 month)
Action Steps
 Deficiencies
 Corrected ASAP
 Memos
 Sent to PI, Department Chair, Asst.
RCO, RCO
 10 days to resolve problems and
reply
 Three memos in 12 months
 Radiation Control Committee takes
direct action with PI and
Department Chair
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Questions?
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