Agenda 9/29 Cell Organelle QUIZ Pass back biomolecule quiz – Discuss corrections and retakes for those who failed to get to a 70 possibly. Notes: The Cell Membrane and Transport. Homework over the cell membrane due next class. The Cell Membrane and Transport Objective Identify, investigate and explain cellular processes – Transport of molecules across the cell membrane Three names Phospholipid bi-layer Cell membrane Plasma membrane What does the cell membrane do? Regulates what enters & leaves the cell Provides protection Provides support GOAL: HOMEOSTASIS What does the cell membrane look like? How does the cell membrane regulate what enters and leaves the cell? Semi-permeable – Lets some things in and out of the cell, but not all things Passive Transport – does NOT require energy Active Transport – does require energy (ATP) Passive Transport - Diffusion Movement of particles (food) from HIGH concentration to LOW Goal = equilibrium or balance of molecules both inside & outside the cell (HOMEOSTASIS) Molecules flow through the lipid bi layer What does diffusion look like? Passive Transport – Facilitated Diffusion For molecules that are too big to diffuse directly through the cell membrane What is Facilitated Diffusion? Membrane proteins will allow some molecules (like sugar) in or out of the cell that are too big to easily diffuse through the cell membrane. Still moving from a high concentration to a low concentration… NO ENERGY REQUIRED! Every time a cell’s environment changes… Water is moving from a higher concentration to a lower concentration. The name of this process is… Osmosis The diffusion of water. Just like the iodine diffused across the dialysis membrane, water moved across the egg’s membrane. Objective Identify, investigate and explain cellular processes – Transport of molecules across the cell membrane Active Transport Movement across the cell membrane from a LOW concentration to a HIGH concentration. Requires energy (ATP) One type uses membrane proteins to push large molecules across the semi – permeable cell membrane. What does Active Transport look like? Image from “The Virtual Cell Textbook” http://www.ibiblio.org/virtualcell/textbook/chapter3/cmf1c.htm Cell Transport: Moving Across the Cell Membrane Endocytosis, Exocytosis endo: inside Exo: outside Cyto: cell Help for the large: Both designed to move a large substance or a large amount of substance. The cell membrane is flexible and can change shape to form a pocket. Both require energy (ATP) Cell Transport: Moving Across the Cell Membrane Endocytosis The membrane folds inward, surrounds the material, and then fuses together. The bubble created with materials is called a vesicle. Materials are usually transported to the lysosome. Exocytosis Vesicle transported to cytoplasm, vesicle fuses with cell membrane, and then releases its contents outside the cell Objective Identify, investigate and explain cellular processes – Transport of molecules across the cell membrane Osmosis and Tonicity Tonicity refers to the total solute concentration of the solution outside the cell. What are the three types of tonicity? 1) 2) 3) Isotonic - equal Hypotonic – lower; under Hypertonic – higher, over Isotonic concentration of water (solvent) outside the cell is the same as the concentration inside the cell. What will happen to a cell placed in an Isotonic solution? The cell will have no net movement of water and will stay the same size. Ex. Blood plasma has high concentration of albumin molecules to make it isotonic to tissues. Hypotonic concentration of water (solvent) outside the cell is higher than the concentration inside the cell. What will happen to a cell placed in a Hypotonic solution? The cell will gain water and swell. If the cell bursts, then we call this lysis. (Red blood cells = hemolysis) In plant cells with rigid cell walls, this creates turgor pressure. Hypertonic concentration of water (solvent) outside the cell is lower than the concentration inside the cell. What will happen to a cell placed in a Hypertonic solution? The In cell will lose water and shrink. plant cells, the central vacuole will shrink and the plasma membrane will pull away from the cell wall causing the cytoplasm to shrink called plasmolysis. Review Tonicity What will happen to a red blood cell in a hypertonic solution? What will happen to a red blood cell in an isotonic solution? What will happen to a red blood cell in a hypotonic solution? Eggs-periment The egg without its shell is a cell with a semi-permeable membrane Cell in Distilled Water What will happen and WHY???? Cell in Corn Syrup What will happen and WHY???? Objective Identify, investigate and explain cellular processes – Transport of molecules across the cell membrane