OHS026
Safe Work Procedure
Faculty/Division
Medicine
Document number
SOMS GBD SWP 003
School/ Divisional Unit
School of Medical Sciences
Initial Issue date
13-03-2009
Current version
1.0
Current Version
Issue date
20-04-2010
Next review date
20-04-2013
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of this
form.
Safe Work Procedure Title and basic description
Use bio-centrifuge centrifuge
Title:
Description: Separate small volumes during RNA and protein extraction
Associated risk assessment title and location: RA handle human tissue 020, RA waste management 015 and RA centrifuge use 018
located in room 209B.
Describe the activity or process
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Inspect rotor and lid for any damage before using
Turn on the bio-centrifuge using the switch on the right hand side
Set the temperature using the up and down keys under the temperature sign
Set the speed using the up and down keys under the rpm sign
Set the time using the up and down keys under clock sign
Place tubes into the rotor, make sure that all tubes are balanced correctly
Screw the lid onto rotor before closing the centrifuge
Close the centrifuge lid
Press start
Open the centrifuge lid and remove the tubes when it finished
Make sure the rotor is clean and no solution has been spilt on the rotor
Turn off the power
Wipe contaminated centrifuge and bench area with 70% alcohol
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc
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Spray bottle of 70% alcohol
Lab gown, safety glasses, disposable gloves, close shoes
Centrifuge, sample tubes
List potential hazards and risk controls including
specific precautions required
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Damage to the rotor or lid can cause failure of centrifuge – inspect for damage before start
Potential electrical hazard – do annual inspection to electrical equipment and ensure inspection tag is current
Not stable centrifuge caused by not correctly balanced tubes – train all users to ensure that everyone knows how to
balance the centrifuge. Make sure the tubes are balanced before start.
Spills from unsecured lid – train all users in the correct way to use centrifuge and always ensure the lid closed before start
Contamination from spills – Do not open the centrifuge lid until it has completely stopped spinning, wear gloves, mask and
lab gown, all the tubes are closed properly before centrifuge and spills should be cleaned immediately with 70% alcohol
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Page 1 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007
List emergency shutdown instructions
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Cetrifuge has “OFF” switch that can be used in emergency.
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Shut all electrical equipment off at power point in case of emergency.
List clean up and waste disposal requirements
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Wipe down all spills with 70% alcohol
Collect the liquid waste into waste bottle, dispose the tubes, gloves and tips in biological waste
List legislation, standards and codes of practice used
in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Australia Dangerous Goods Code
Code of Practice for the Labelling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
Australian Standard AS2243.6-1990. Safety in laboratories. Part 6: Mechanical Aspects.
Australian Standard AS2243.7-1991. Safety in laboratories. Part 7: Electrical Aspects.
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
Safe Work Procedure Form (OHS026)
UNSW Hazardous Waste Disposal Procedure
Supervisory approval, training, and review
Supervisor: Prof E Burcher
Signature:
Plant custodian:
Signature
List competency required – qualifications, certificates, licencing, training - eg course or instruction:
SWP review date: 20-04-2013
Responsibility for SWP review: Fei Shang
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Page 2 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007