Centrifuge_SOP

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Sport and Exercise Sciences Research Institute

Standard Operating Procedure

Use of Bench Centrifuge (refrigerated) (SS/SOP0017)

Centrifugation is a process which separates two or more components, depending on their density - the low density material will be at the top.

Only trained personnel shall be allowed to use the centrifuge

Supervisor and/or technician are responsible for ensuring that personnel are trained to use the centrifuge

User must wear laboratory coat and gloves

Centrifuge is only used at speed <4000 rpm

Centrifuge tubes with any defects must be discarded

Tubes must be properly balanced in the rotor, using balance, and, if need be use a tube with water to balance your other tube

Never fill centrifuge tubes above the maximum recommended by the manufacturer

Only use correctly fitting tubes/buckets

Set the temperature, speed and time using the dial and select button on the centrifuge

Centrifuge lid will not open until the rotors have completely stopped

User shall check cleanliness and visible damage before using

PAT testing shall be carried out on an annual basis

Never use explosive or highly flammable chemicals

Report all spills or faults to supervisor or technical staff

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