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COBRE Epigenetics Bioinformatics Core
Effective Date: 12/17/2014
Sample Submission Form
Revision No.: 02
[ECC Use Only]
Core Project ID: Click here to enter text.
Instructions: Please complete Sections I-IV. Incomplete or missing information may delay work for
your project. DNA samples submitted for sequencing will be quantified using the Qubit 2.0
fluorometer. RNA samples submitted for sequencing will be quantified using the Qubit 2.0
fluorometer and RNA integrity assessed using the Agilent 2100 Bioanalyzer. All prepared sample
sequencing libraries will be quality checked using the Agilent 2100 Bioanalyzer and KAPA Library
Quantification qPCR kit.
SECTION I. User Information
Name:
Principal Investigator Name:
Project Title:
E-mail:
Phone:
Core User Information
Click here to enter text.
Click here to enter text.
Click here to enter text.
Click here to enter text.
Click here to enter text.
SECTION II. Sample Information
Library Preparation Kit Used
Sample Type
(Please list the kit type below)
(Please check below)
User-prepared library
Genomic DNA
cDNA
Total RNA
mRNA
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Click here to enter text.
DNAase-treated?
Yes ☐ No ☐
Yes ☐ No ☐
Sample Preparation Information
(Please complete the following)
Sample Species: Click here to enter text.
Purification Method: Click here to enter text.
Elution Buffer: Click here to enter text.
Click here to enter text.
Additional Comments/Notes:
Have you performed any QC?1
1
Yes
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No
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If you performed QC on your samples, you must provide a copy of your results or work for
your project will be delayed!
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COBRE Epigenetics Bioinformatics Core
Effective Date: 12/17/2014
Sample Submission Form
Revision No.: 02
SECTION III. Application
Application Type
(Please check below)
RNA-Seq2
ChIP-Seq
Small RNA Sequencing
Small Genome Sequencing
Infinium Methylation Array3
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Only small genomes (i.e. Bacteria/Fungi) can be
sequenced on the MiSeq. Larger genomes (i.e.
C.Elegans/Drosophila/Human/Mouse) will be sent to
UMGC for sequencing on the HiSeq.
3
Infinium Methylation Arrays will be sent to UMGC.
Library Prep:
TruSeq RNA
TruSeq Small RNA
TruSeq ChIP
TruSeq DNA PCR-Free
Nextera XT DNA
Nextera DNA
Other (List below)
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[ECC Use Only]
Sequencing Run Type
Single Read ☐
Paired End Read ☐
Sequencing Run Length:
75 bp ☐
150 bp ☐
250 bp ☐
300 bp ☐
Reagent Kit:
V2 50 cycles
V2 300 cycles
V2 500 cycles
Micro V2 300 cycles
Nano V2 300 cycles
Nano V2 500 cycles
V3 150 cycles
V3 600 cycles
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COBRE Epigenetics Bioinformatics Core
Effective Date: 12/17/2014
Sample Submission Form
Revision No.: 02
SECTION IV. Sample List
NOTE: For DNA samples, please submit ≥ 1 µg of high quality DNA, concentration > 50 ng/µL; for RNA
samples, please submit ≥ 1 µg of total RNA, concentration of at least 50 ng/µL
[ECC Use Only]
Sample Name
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Concentration
(ng/µL)
Sample
Volume
(µL)
Adapter
Index Used
(User-prepared
libraries only)