PROJECT TITLE
DATABASE STUDY NAME:
We need a shortened version of your complete study name. This will be the name used to identify your
study at the HTSF, you will submit samples under this study name, this name will be on all sample tubes
submitted and created at the HTSF during processing. We suggest between 5-10 characters long. It can
be both alpha and numeric.
PRINCIPAL INVESTIGATOR
Name:
Title:
Department:
Institution:
UNC
Address:
E-mail:
ONYEN
Other (Please specify):
Telephone:
CO-INVESTIGATOR(S) AND OTHER PERSONNEL INVOLVED IN PROJECT
Name
Email
ONYEN
Telephone
PROJECT INFORMATION (check all that apply; this information may help you qualify for extra support from the
cores and the biostatistics/informatics group)
This is clinical research (as defined by the NIH)
This is translational research
The UNC Genomics Core Laboratories are not HIPAA compliant and cannot accept samples with
identifying patient information.
FUNDING SOURCES
Is one or more members of the study part of:
UNC
LCCC, Lineberger Cancer Center
Please list the following information for the funding source(s) you plan to use for this project. We
do not need the funding account number at this time. You may list more than one account if
necessary when you submit your samples to the HTSF.
Accountant Name
Department
E-mail
Telephone
SAMPLE DETAILS:
Species:
Human
Mouse
Other (please specify:
)
RNA/DNA source(s) (tissue/ cell type/ FFPE/ Frozen):
Estimated RNA/DNA (per sample):
units:
µg
ng
pg
KIND OF SAMPLE:
ChIP DNA (specify antibody used):
Genomic DNA
Amplicon
Unfragmented cDNA
Fragmented cDNA
cDNA
Total RNA
mRNA
Ribosomal RNA depleted RNA
Small RNA, purified
Amplicon
Other (specify):
LIBRARY SERVICES REQUESTED (check all that apply and circle or highlight desired format)
QA/QC Service:
DNA QC (Nanodrop, Qubit)
RNA QC (Nanodrop, Bioanalyzer, Qubit)
Library Preparation Services:
Illumina Library preparation
DNA
Final Library type:
DNA SEQ
Exome Capture
Sure Select
Total RNA
RNA Tru Seq
Final Library type:
Total RNA
Non Stranded
Stranded
mRNA
Non Stranded
Stranded
ribosomalRNA DepletedRNA
CHiPSeq
Amplicon / 16s metagenomics
Other Illumina Library services
PacBio
Ion Torrent
SEQUENCING SERVICES REQUESTED (check all that apply and circle or highlight desired format)
DNA Sequencing – please see specifics below
RNA Sequencing – please see specifics below
DNA Methylation
Small RNA Sequencing
SEQUENCING PLATFORM(S) (check all that apply)
Illumina HiSeq2000 sequencing
Illumina HiSeq2500 sequencing
Illumina MiSeq sequencing
PacBio
Ion Torrent
SEQUENCING SERVICES REQUESTED (check all that apply and circle or highlight desired format)
Single End
Paired End
Multiplex Sequencing (pooled libraries – aka more than one library in a lane)
ADDITIONAL REQUIRED SEQUENCE INFORMATION :
Libraries have Dual Barcodes?
Please follow specific instructions of listing Dual barcodes on the submission
manifest in order for De-mulitplexing to work properly
Custom Primer required?
You will be required to submit the primer at the time of the sample submission.
Clearly labeled with primer name on the tube and indicated on the submission manifest.
Control lane required
Requested Read Length (sequencing cycle #):
Number of lanes requested to run / pool OR single sample:
PROJECT SIZE AND TIMETABLE
Overall estimate number of project samples
Samples are already available and we are ready to start as soon as possible.
We expect to be ready to start on
(specify estimated date)
Comment:
DATA ANALYSIS:
Data analysis – assistance requested for analysis. This can include suggesting software, basic training to
analyze your data and suggestions of possible UNC faculty/ staff to assist.
Data analysis – Project will do it’s own analysis, Please people responsible for analysis
Name
Email
ONYEN
Telephone
PROJECT DESCRIPTION
Please insert brief statements below each of the following headings.
HYPOTHESIS/SPECIFIC AIMS BACKGROUND AND SIGNIFICANCE (One paragraph maximum
please.)
EXPERIMENTAL DESIGN
(optional)
List the factors that characterize the experimental groups (e.g., genotype, treatments, age, time,
cell lines, tissue type, etc.)
Fill out the following table (see example below).
Group
A
B
C
D
E
F
G
Group Description
#
Samples
Total Number of Samples
ANALYSIS (required)
What analyses are of interest? (e.g., compare Group A to Group B, determine the effects of the
transgene, and analyze the interaction between the transgene and the treatment)
Comments, Special Considerations and/or Additional Instructions: