GSCI 2013: Group 2-Cell and
Developmental Biology
Seth Jones, University of Kentucky
Karen Maruska, Louisiana State University
Joy Davis, Baton Rouge Community College
Sabrice Guerrier, Millsaps College
Naila Mamoon, Millsaps College
Facilitator: Peter Cavnar, University of West Florida
CELLULAR
RESPIRATION
Context
What kind of course is unit designed for?
mid-level
How long is unit?
1 week
When will the unit be used in the course? After
students have learned basic chemistry, energetics, redox
rxn, protein structure, cell membrane, transport, and
organelles
Class size? Should be fine for all ranges
Learning goal
• Understand how the energy from electrons in glucose is
converted to chemical energy in ATP during oxidative
phosphorylation.
Learning outcomes
• Explain the role of oxygen in cellular respiration
• Explain how the H+ gradient across the inner mitochondrial
membrane is generated and how it drives ATP synthesis
• Predict the effects of various drugs on oxidative
phosphorylation
How long can you hold your breath?
Brainstorm: So why do you think we
need oxygen?
Loss of hydrogen atoms
(becomes oxidized)
C6H12O6
6 O2
6 CO2
6 H2O
Glucose
Gain of hydrogen atoms
(becomes reduced)
ATP
+ Heat
CYTOPLASM
NADH
Electrons
carried by NADH
NADH
Glycolysis
Pyruvate
Glucose
Pyruvate
Oxidation
Citric Acid
Cycle
FADH2
Oxidative
Phosphorylation
(electron transport
and chemiosmosis)
Mitochondrion
ATP
ATP
ATP
Substrate-level
phosphorylation
Substrate-level
phosphorylation
Oxidative
phosphorylation
Figure 6.10
H+
Intermembrane
space
H+
H+
H+
H+ Mobile
electron
carriers
Protein
complex
of electron
carriers
H+ ATP
synthase
IV
I
II
FADH2
Electron
flow
NADH
Mitochondrial
matrix
H+
H+
III
Inner mitochondrial
membrane
H+
NAD+
FAD
2 H+
1
2 O2
H2O
H+
ADP
P
ATP
H+
Electron Transport Chain
Oxidative Phosphorylation
Chemiosmosis
Welcome to Club Mitochondria
• Instructions: Pair with a
partner then identify the
characters within the
analogy that correspond
to the following:
• 1. Electrons
• 2. Protons
• 3. Electron transport chain (ETC)
• 4. ATP synthase
• 5. Mitochondrial Matrix
• 6. Intermembrane space
What is the layout in Club Mitochondria?
Main entrance
Female Patron
Foyer
Dance
Floor
Male Patron
Consider a scenario where the electron transport
chain ("dance line") is disrupted in the
mitochondria.
What is most likely to happen to the H+
concentration ([H+]) in the intermembrane space?
• A. Decrease in [H+]
• B. Increase in [H+]
• C. No change
• D. I don't know
Consider a scenario where the electron transport
chain ("dance line") is disrupted in the
mitochondria.
What change would you expect in ATP
concentration in the cell?
• A. Decrease in [ATP]
• B. Increase in [ATP]
• C. No change
• D. I don't know
Figure 6.11
Inhibitors of cellular respiration
Rotenone
Cyanide,
carbon monoxide
H+
H+
H+
Oligomycin
ATP
synthase
H+
H+ H+ H+
DNP
FADH2
FAD
NAD+
NADH
1
O
2 2
2 H+
H+
Your homework: List the signs and symptoms in victims
with acute cyanide poisoning AND we would like you to
extend the analogy to include oxygen and ATP
H2O
ADP
P
ATP