11.2 Movement Understanding: - Bones and exoskeletons provide anchorage for muscles and acts as levers - Movement of the body requires muscles to work in antagonistic pairs - Synovial joints allow certain movements but not others - Skeletal muscle fibres are multinucleate and contain specialised endoplasmic reticulum - Muscle fibres contain many myofibrils - Each myofibril is made up of contractile sarcomeres - The contraction of the skeletal muscle is achieved by the sliding of actin and myosin filaments - Calcium ions and the proteins tropomyosin and troponin control muscle contractions - ATP hydrolysis and cross-bridge formation Applications: - Antagonistic pairs of muscles in an insect leg Nature of science: - Fluorescence was used to study the cyclic interactions in muscle contraction Skills: - Annotation of a diagram of the human elbow. - Drawing labeled diagrams of the structure of a sarcomere - Analysis of electron micrographs to find the state of contraction of muscle fibres Bones and exoskeletons Provide anchorage for muscles and act as levers. Levers change the size and direction of forces. In a lever - Effort force - Pivot point (fulcrum) - Resultant force Different positions of these = different lever classes Class 1 lever Nodding head backwards Fulcrum (pivot) in between the effort force (muscle) and the resultant force (load) Class 2 lever Resultant force (load) is in between effort force (muscle) and fulcrum (pivot) Standing on tip toes Class 3 lever Bending fore arm Effort force (muscle) in between fulcrum (pivot) and load. Antagonistic muscles Movement of the body requires muscles to work in pairs When one contracts, another relaxes. Biceps and triceps are antagonistic muscles – how do they bend and extend your arm? Annotate the human elbow Label the following onto your elbow: - Humerus - Tricep Describe the role of - Bicep each - Joint capsule Add in any drawings - Synovial fluid you need to of muscles - Radius - Cartilage - Ulna Ludo Loki Range of movements at shoulder Structure of joint determines the movements Movement Flexion Extension Abduction Adduction Outward rotation Inward rotation Describe it Stick man drawing Flexion and extension at shoulder Outward and inward rotation Types of joints Different types of joints allow different movements Describe the different types of joints (Hinge, pivot, ball and socket) What movements do each of them allow? Types of joints Hinge joint – knee/elbow Flexion and extension Pivot joint - neck Rotations Ball and socket joint – hip/shoulder All movements! Structure of muscle fibres Muscles attached to bone = skeletal muscles (by tendons) View using microscope = stripes are visible Striated muscle Types of muscles Type of muscle Striated Smooth Cardiac What does that muscle do? Where is that muscle? Drawing Striated muscle Composed of bundles of muscle cells called muscle fibres Muscle fibres much longer than typical cells Many nuclei present Sarcoplasmic reticulum (modified endoplasmic reticulum) conveys signal to contract all parts of the muscle fibre at once Large numbers of mitochondria to produce ATP for contractions Myofibrils Parallel elongated structures Alternating light and dark bands In each light band is a disc shaped structure called the Z line Myofibrils Each myofibril is made up of contractile sarcomeres Between one Z line and the next is a sarcomere Sarcomere Draw and label your own diagram of a sarcomere - Thick myosin filaments – must have heads - Thin actin filaments - Light band (also indicate the range) - Dark band (also indicate the range) - Z line - Sarcomere Skeletal muscle contractions Thick myosin filaments pull the thin actin filaments inwards towards the centre of the sarcomere Shortens the sarcomere and the overall length of the muscle fibre Muscle contractions Under the microscope – must determine the state of skeletal muscle contractions Compare the relaxed and contracted pictures Control of contractions Contraction of skeletal muscle occurs by the sliding of actin and myosin filaments Myosin filaments have heads that can bind to special sites on actin filaments, creating cross bridges. Control of contractions Use energy from ATP and exert a force to move filaments. Heads are spread along myosin filaments regularly, and binding sites are spread along actin filaments – many cross bridges can form at once. Control of contractions Relaxed muscle = tropomyosin protein blocks binding sites on actin Motor neuron sends signal to contract muscle fibres Sarcoplasmic reticulum releases calcium ions Calcium ions bind to troponin protein which causes tropomyosin to move – exposing actin binding sites Myosin can then bind to actin to start contraction Sliding filaments ATP hydrolysis and cross bridge formation must occur for filaments to slide. Complete the labels on your sheet: 1. Myosin filaments have heads which form crossbridges when they are attached to binding sites on actin filaments Sliding filaments 2. ATP binds to the myosin heads and causes them to break the cross-bridges by detaching from the binding sites 3. ATP is hydrolysed to ADP and phosphate, causing the myosin heads to change their angle. The heads are said to be cocked in their new position as they are storing potential energy from ATP Sliding filaments 4. The heads attach to binding sites on actin that are further from the centre of the sarcomere than the previous sites 5. The ADP and phosphate are released and the heads push the actin filament inwards towards the centre of the sarcomere – this is called the power stroke Sliding filaments ATP causes breaking of cross-bridges by attaching to the myosin heads, causing them to detach from the actin binding sites Hydrolysis of ATP to ADP and phosphate provides energy for the myosin heads to swivel outwards away from the centre of the sarcomere and attach to a new binding site. Sliding filaments Stages continue until the motor neuron stops sending signals to the muscle fibres Calcium ions then pumped back into the sarcoplasmic reticulum. Actin binding sites are covered again by tropomyosin. Muscle relaxes. Labeling your diagram Use the powerpoint on my website and the video links too. Label in each step that occurs during a muscle contraction from start (signal for contraction) to end (relaxed muscle) Use the videos: Action potentials and muscle contractions Break down of ATP… Myofilament contraction Include the following key words: - ATP - ADP + phosphate - Cross bridges - Sliding filaments - Tropomyosin - Troponin - Actin - Actin binding sites - Contraction - Calcium ions