Chapter 7 A View of the Cell Chapter 7.1 The Discovery of Cells Hickox: Baker High School 1 Chapter 7 p.170-191 (text) pp. 68 – 88 (Reading Essentials) Terms: • Organelle • Ribosome • Cellulose • Cell membrane • Smooth ER • Nucleolus • Chloroplast • Cell wall • Mitochondria • Cytoskeleton • Vacuole • Golgi apparatus • Nucleus • Rough ER • Cytoplasm Terms: • Hooke • Schleiden • Schwann • Virchow • van Leeuwenhoek • Light microscope • SEM • TEM Terms: • Eukaryotic • Prokaryotic Hickox: Baker High School Unit 1 2 Objective 4.0 • Describe similarities and differences of cell organelles, using diagrams and tables. •Identifying scientists who contributed to the cell theory (Examples: Hooke, Schleiden, Schwann, Virchow, van Leeuwenhoek) •Identify various technologies used to observe cells (Examples: light microscope, scanning electron microscope, transmission electron microscope) •Distinguish between Prokaryotic and Eukaryotic cells. Core Concept: Cells have particular structures that underlie their functions. Every cell is surrounded by a membrane that separates it from the outside world. The cell membrane encloses the cell and separates the cell interior, called the cytoplasm, from its surroundings. The cell membrane also regulates what enters and leaves a cell. Inside the cell is a concentrated mixture of thousands of different molecules which form a variety of specialized structures that carry out such cell functions as energy production, transport of molecules, waste disposal, synthesis of new molecules, and the storage of genetic material Inside the cytoplasm are many structures, often suspended in a system of microscopic fibers called the cytoskeleton. All cells have ribosomes. Ribosomes are the cellular structures on which proteins are made. Hickox: Baker High School 3 Core Concept: The smallest and simplest cells are prokaryotes. A prokaryote is a single-celled organism that lacks a nucleus and other internal compartments. The first cells with internal compartments were primitive eukaryotic cells. A eukaryote is an organism whose cells have a nucleus. The nucleus is an internal compartment that houses the cell’s DNA. Other internal compartments, or organelles, enable eukaryotic cells to function in ways different from bacteria. An organelle is a structure that carries out specific activities in the cell. Core concept: Progress in science and invention depends heavily on what else is happening in society, and history often depends on scientific and technological developments. Science often advances with the introduction of new technologies. Solving technological problems often results in new scientific knowledge. New technologies often extend the current levels of scientific understanding and introduce new areas of research. Hickox: Baker High School 4 A View of the Cell Hickox: Baker High School 5 The Discovery of Cells Essential Question: How did the microscope change everything? ______________________ _______________________________________ _______________________________________ _______________________________________ Hickox: Baker High School 6 The Discovery of Cells 1. The invention of the microscope changed everything. 2. Before the microscope people believed that diseases were caused by curses and supernatural spirits. 3. The microscope opened up a whole new world, scientists were able to view and study cells. 4._________ are the basic units of living organisms. 5. In the 1600’s van Leeuwenhoek used a simple light microscope.(one lens, natural light) 6. Today we use the ___________ __light microscope (multiple/series of lenses) Hickox: Baker High School 7 p. 68 Cork cells “Cells are the (7.)basic units of all forms of life” Identification of Cells: COMPOUND LIGHT MICROSCOPE 8. Plant tissue (cork) – Robert Hooke, 1665 9. Animal tissue (van Leeuwenhoek, 1674) 10. All plants and animals are composed of cells was a theory proposed by: ( Schleiden and Schwann ) Hickox: Baker High School 8 The Discovery of Cells (p. 68) • Schleiden, in 1830, a German scientist studied plants and concluded: “All plants are composed of cells”. In 1838 Schleiden proposed that all (11) plants are composed of cells; together with his friend Theodor Schwann , with Schleiden formulated the (12) cell theory of life. Schleiden also found that certain fungi live on or within the roots of some plants. This relationship between fungi and plants, called (13) mycorrhiza. In 1839 Schwann proposed that all organisms are composed of cells. Schwann, made similar observations on animals. Schleiden and Schwann developed one of the important theories in Biology, the (14) ______________ theory. Hickox: Baker High School 9 The Discovery of Cells (p. 68) 15. Robert Hooke used a compound light microscope and looked at cork from oak trees, called the structures __________, because they looked like small rooms in monastery. 16. The _________ microscope does not use light but a beam of electrons. 17. Can magnify structures up to _________ times 18. There are three types of electron microscopes: Hickox: Baker High School 10 What is cell theory? (p.69) • Schleiden, and Schwann, and other scientists led to the cell theory. 1. All living things are made of one or more cells 2. Cells are the basic units of structure and function in living things. 3. All cells come from other cells. Hickox: Baker High School 11 Two Basic Cell Types Essential Question: ? How are prokaryotic and eukaryotic cells similar and different? _______________________________________ _______________________________________ _______________________________________ _______________________________________ Hickox: Baker High School 12 The Discovery of Cells THE TWO BASIC CELL TYPES (p. 69) 1) Those with membrane bound structures called ____________. The cell type that have organelles that are surrounded by membranes are called _____________ Video: eukaryotic cells cells. Video: Introductory to cells Hickox: Baker High School 13 THE TWO BASIC (p. 69) CELL TYPES 2) Those with out a membrane surrounding the organelles • Cells of most unicellular organisms, such as __________, are __________________. • Most multi-cellular organisms are eukaryotic. • Some eukaryotic are single cell organism, an example is yeast. Hickox: Baker High School 14 Video: Prokaryotic cells The Discovery of Cells Video: Nucleus • Central organelle (little organ) is called a ____________ that controls all of the cell’s activities. This is found in Eukaryotic cells. • Prokaryotes cells like, bacteria do not have a nucleus, but both have DNA. Hickox: Baker High School 15 A (3)prokaryotic cell does not have internal organelles surrounded by a ____________. membrane Most of a prokaryote’s metabolism takes place in the cytoplasm. 2. DNA 1. Ribosomes 3. Plasma membrane 4. Cell wall 5. Flagella Hickox: Baker High School This eukaryotic cell from an(4) animal or plant has distinct membrane-bound organelles that allow different parts of the cell to perform different functions. 1. Nucleus 2. Nucleolus 3. Chromosomes 4. Plasma membrane 5. Organelles 17 Hickox: Baker High School Organelles of a cell: Draw on separate sheet of paper G. D. L. K. J. E. I. M. A. H. Figure 7-6 C. B. Cell Alive Web Site • http://www.cellsalive.com/cells/cell_model.htm Chapter 7 A View of the Cell Chapter 7.2 The PLASMA MEMBRANE Hickox: Baker High School 20 The Discovery of Cells MAINTAINING A BALANCE (copy p. 71) 1. All living cells must maintain a balance regardless of internal and external conditions or Else! This balance is called ________________ 2. The _______________ is the flexible boundary of a cell that separates the cell from its surroundings. It allows nutrients to enter and waste to be removed. This balance is called ___________. Hickox: Baker High School 21 Maintaining a Balance (p. 71) 3. The plasma membrane maintains homeostasis by a process that allows some molecules into the cell and keeps others out. 4. This is called ___________ ____________. 5. Some molecules, such as water freely enter. Other molecules are only admitted at certain times and in limited amounts. Others are not allowed at all. Hickox: Baker High School 22 Plasma Membrane Water Hickox: Baker High School 23 Structure of the Plasma Membrane Structure of the Plasma Membrane (p. 71) 6. Lipids or fats are a large molecules. Phospholipids are made of glycerol, two fatty acids, and a phosphate group 7. The plasma membrane is composed of a phospholipid _________. 8. The bilayer has two layers of phospholipids back to back. Hickox: Baker High School 24 Phosphate Group Glycerol Backbone The lipids in a plasma membrane have a glycerol backbone, two fatty acid chains, and a phosphate group. Two Fatty Acid Chains Hickox: Baker High School 25 The Phospholipid Bilayer (p. 72) The phosphate group is pointing out and is polar. The polar heads face out and the non-polar tails face in A A A A A A A Hickox: Baker High School 26 Two Basic Cell Types Essential Question: What is so important about the Fluid Mosaic Model? _______________________________________ _______________________________________ _______________________________________ _______________________________________ Hickox: Baker High School 27 The phospholipid bilayer 9. Water is the key component in living organisms, inside and out. • What makes this bilayer work is the outer structures are water friendly (solubility) on the outsides of the membrane. The fatty acid tails are 10. not water friendly and water will not pass easily through. 11. Think of it like fat and water mixed together, they don’t easily mix. 12. Other molecules that are water soluble also have difficulty moving through. Hickox: Baker High School 28 Lipid Bilayer is a Fluid Mosaic Model (p.72) • It is fluid because the phospholipids 13. move within the membrane 14. Transport proteins also move along phospholipids. 15. Cholesterol helps to stabilize the phospholipids, preventing tails sticking together. • Proteins help form the selectively permeable that 16. regulates which molecules enter and which molecules leave a cell. Hickox: Baker High School 29 Fluid Mosaic Model (p.72) • Proteins that span the entire width of the bilayer are called 17. _____________ proteins. • These proteins move needed substances or waste materials through the plasma membrane. • Other proteins stick out of the plasma membrane and help protect from infection. (write into notes) • If the movement goes against natural flow, it is called 18. __________ transport. It the movement goes with natural flow it is called 19. __________ transport Hickox: Baker High School 30 Fluid-Mosaic Model Hickox: Baker High School 31 Crossing Plasma Membrane Hickox: Baker High School 32 Diffusion 20. Diffusion - Movement of molecules from a higher to a lower concentration until equilibrium is reached. – Down concentration gradient 20. A solution contains a solute (solid) and a solvent (liquid). Hickox: Baker High School 33 Diffusion Hickox: Baker High School 34 Osmosis 21. Osmosis - Diffusion of water across a differentially (selectively) permeable membrane due to concentration differences. – Osmotic pressure is the pressure that develops due to osmosis. • The greater the osmotic pressure, the more likely water will diffuse in that direction. Hickox: Baker High School 35 Osmosis Hickox: Baker High School 36 Osmosis 22. Isotonic Solution - Solute and water concentrations both inside and outside the membrane are equal. 23. Hypotonic Solution - Solution with a lower concentration of solute than the solution on the other side of the membrane. – Cells placed in a hypotonic solution will swell are called 24. Lysis Hickox: Baker High School 37 Osmosis 24. Hypertonic Solution - Solution with a higher concentration of solute than the solution on the other side of the membrane. – Cells placed in a hypertonic solution will shrink are called: 25. Plasmolysis Hickox: Baker High School 38 Transport by Carrier Proteins Proteins are use to move particles against the natural flow in a process called 26. Active Transport Hickox: Baker High School 39 Membrane-Assisted Transport 27. Exocytosis – Waste products moved out of a cell from a vesicle. Hickox: Baker High School 40 28. EndocytosisFood brought into a cell. Hickox: Baker High School 41 Chapter 7 A View of the Cell Chapter 7.3 Eukaryotic Cell Structure Hickox: Baker High School 42 Nucleus and cell control Nucleolus Chromatin Nuclear Envelope Hickox: Baker High School 43 The Nucleus NUCLEAR PORES NUCLEOLUS Largest (29) Organelle in the Cell Surrounded by a double membrane (30) NUCLEAR ENVELOPE Contains chromosomes in the form of (31) CHROMATIN contain (32) DNA Hickox: Baker High School 44 7.1 Eukaryotic Cell Structure 7.3 Cellular Boundaries (p. 74) Remember: The fluid mosaic model describes the plasma membrane as a (34) flexible boundary. In plants cells, fungi, bacteria and some protists have an additional boundary, the (35) ______ _______. Cell Wall Fairly (36) rigid structure located (37) outside the plasma membrane Provides additional (38) support and (39)protection Hickox: Baker High School 45 The cell wall The (40) cell wall is a fairly rigid structure located (41) outside the plasma membrane that provides additional support and protection. Video: cell wall and cell membrane Hickox: Baker High School 46 Cell Walls in Plants • Cell wall is made of (42) cellulose Letter “e” Lab, cellulose in paper • Hickox: Baker High School 47 The Discovery of Cells Eukaryotic Cell Structure 7.3 Cellular Boundaries Cell membranes are(43) flexible,(44) selectively permeable structures, cell membranes are composed of (45) phospholipid bilayer with protein structures to transport non-soluble structure through the membrane. Inside of cell Hickox: Baker High School Plasma membrane Outside of cell 48 The Discovery of Cells Eukaryotic Cell Structure 7.3 Chromatin The Nucleus and Cell Control (p.74) • Contains the directions to make (46)_________. • Proteins are needed throughout the cell and the nucleus controls the blue print to make these proteins. • The master set of directions for making the proteins in contained in (47) __________ • Chromatin are strands of the genetic material, (48)________. Hickox: Baker High School 49 Two Basic Cell Types Essential Question: The cell operates like a little factory with small organs. Name some of these organelles and their functions. _______________________________________ _______________________________________ _______________________________________ _______________________________________ Hickox: Baker High School 50 Chromatin and Chromosomes (49) Chromatin are granular material visible in nucleus and contains (50)DNA bound up with protein During cell division the chromatin condenses to form (51)Chromosomes which are distinct threadlike structures containing the genetic information. Hickox: Baker High School 51 How the Information, to make proteins gets from the nucleus to the sites of protein synthesis (Ribosomes) Hickox: Baker High School 52 The Discovery of Cells Eukaryotic Cell Structure 7.3 • • • • • When a cell divides- (p. 74) The chromatin condenses to form (52)_____________. Within the nucleus is a prominent organelle called the (53)___________. The function of the nucleolus is to make (54) __________. Ribosomes are sites where proteins are manufactured according to the directions of DNA. Ribosomes are not membrane bound. Hickox: Baker High School 53 Assembly, Transport, and Storage 7.3 Ribosomes are made of (55) _____ and proteins. They are not bound by a membrane For proteins to be made, ribosomes must leave the nucleus and enter the cytoplasm and DNA must be (56)________ into RNA. (57)___________ is the clear, gelatinous fluid inside the cell. Ribosomes and translated RNA are transported to the cytoplasm through the nuclear envelope. Hickox: Baker High School 54 Assembly, Transport, and Storage Endoplasmic Reticulum (ER) Ribosomes Hickox: Baker High School 55 Ribosomes (video: making proteins) • Composed of large and small subunits that are used in (58) protein synthesis. Ribosomes are made in (59) nucleolus of the nucleus and are transported through the nuclear pores to the ER. (The places of chemical activity or highway system) • Occur singly and in groups, and may become attached to endoplasmic reticulum. Hickox: Baker High School 56 The Discovery of Cells Eukaryotic Cell Structure 7.3 • The nuclear envelope is a structure that separates the nucleus from the cytoplasm. Assembly, Transport, and Storage (p. 75) The cytoplasm suspends the cell’s organelles. • The cell’s site of chemical reactions is the (60) ___________________ or the _______ (61)__________ are attached to surface of ER. • Areas of the ER that do not have ribosomes is the (62)__________ ER. Hickox: Baker High School 57 Video: Endoplasmic reticulum Hickox: Baker High School 58 Click protein Assembly, Transport, and Storage 7.3 • The ribosome’s job is to make (63) proteins. • Each protein has a particular (64) function. –May be proteins that form part of plasma membrane –or protein released from the cell –or protein floating around in cytoplasm Ribosomes on Rough Endoplasmic Reticulum Hickox: Baker High School 59 Hickox: Baker High School 60 Golgi Apparatus: sorts proteins into packages Packages packs into membrane bound structures called vesicles Hickox: Baker High School 61 Golgi apparatus Hickox: Baker High School Video: Golgi apparatus 62 Vacuoles and storage • • • • • Vacuoles Click for video! Mostly only found in (65) plant cells Cells have membrane – bound compartments, called(66) vacuoles, for (67) temporary storage of materials. The storage for (68) food, enzyme, and water • Lysosomes Organelles that contain (69) digestive enzymes that digestive excess or worn out particles. Hickox: Baker High School 63 • Lysosomes – Membrane-bounded vesicles produced by the (70) Golgi apparatus and are filled with enzymes. – Break down lipids, carbohydrates, and proteins – Break down old (71) organelles that have outlived their usefulness – Remove debris Hickox: Baker High School 64 Vacuole Hickox: Baker High School 65 Vacuoles and storage Vacuoles are membrane-bound spaces used for temporary storage of materials. Notice the difference between vacuoles in plant and animal cells. Animal Cell Vacuole Hickox: Baker High School Plant Cell 66 Energy Transformer • Located in the cells of green plants are (72) _____________. These organelles capture light energy and convert it to chemical energy. • Within the chloroplast are membrane structure that trap the sunlight called (73) ______________. • Stacks of these structures are called (74) _________. Hickox: Baker High School 67 Chlorophyll • Contain green pigment that traps light energy and gives leaves their green color Mitochondria and Energy • Chemical energy generated in chloroplasts, is stored in bonds of sugar until broken down in (75) ______________. Click for • “Energy Houses” video! Hickox: Baker High School 68 Organelles for Support and Locomotion • Cells have a support structure called the (76)_____________ • Forms a framework for the cell like the skeleton for our body. • Contains tiny rods and filaments called (77) ______________, thin hollow cylinders • Smaller solid protein fibers that act as scaffolds are called: (78) ________________ Hickox: Baker High School 69 Cilia & Flagella (79) CILIA and (80) FLAGELLA Hickox: Baker High School 70 Cytoskeleton Section 7-3 Cell membrane Endoplasmic reticulum Microtubule Microfilament Ribosomes Michondrion Hickox: Baker High School 71 Euglena Go to file and play video! Hickox: Baker High School 72 Plant Cell Anatomy Hickox: Baker High School 73 7.3 What’s Inside the Cell Hickox: Baker High School 74 7.2 What’s Inside the Cell Aa a a a a a a aaaaaaaaaaaaaaaaaa a Hickox: Baker High School 75 Comparing Cells ( yes or no for each) Structure _________ Prokaryotic Cells Bacteria Eukaryotic Cells Animal Plant Cell Membrane Cell Wall Nucleus Ribosomes Endoplasmic Reticulum Golgi Apparatus Lysosomes Mitochondria Chloroplasts Cytoskeleton Hickox: Baker High School 76 Comparing Cells (copy chart into notes) Structure _________ Prokaryotic Cells Cell Membrane Yes Yes Yes Cell Wall Yes No Yes Nucleus No Yes Yes Ribosomes Yes Yes Yes Endoplasmic Reticulum No Yes Yes Golgi Apparatus No Yes Yes Lysosomes No Small or None Yes Mitochondria No Yes Yes Chloroplasts No No Yes Cytoskeleton No Yes Yes Bacteria Eukaryotic Cells Animal Plant Hickox: Baker High School 77 Cell Structures 7 - 3 Hickox: Baker High School 78 Figure 7.6 The plasma membrane Figure 7.7 Overview of an animal cell 1. 17. 4. 2. 3. 15. 16. 14. 13. 5. 6. 7. 8. 12. 11. 10. 9. 18., 19. Figure 7.8 Overview of a plant cell 11. 15. 12. 13. 1. 14. 2. 3. 10. 5. 10. 6. 9. 6., 7., 8. Figure 7.9 The nucleus and its envelope Figure 7.x1 Nuclei and F-actin in BPAEC cells Figure 7.11 Endoplasmic reticulum (ER) Figure 7.12 The Golgi apparatus