Gel Electrophoresis Lab
Day 2
Ms. Blalock, Ms. Hartsell, Mr.
Luckman
Do Now
Directions: How does a restriction enzyme know
where to cut?
Today’s Goal
Aim: How can we use DNA evidence and gel
electrophoresis to solve a crime?
Agenda
Activity #1—Restriction Enzyme Review
Activity #2—Lab Materials
Stations
—Lab Demo
—Google Drive Lab report
Restriction Enzyme Review
A cut site is a pattern of DNA that marks the
place where a restriction enzyme will cut.
CC|GG
Restriction Enzyme Review
A cut site is a pattern of DNA that marks the
place where a restriction enzyme will cut.
CC|GG
For example, in our lab, the restriction enzymes
cut in between two CC’s and two GG’s.
Restriction Enzyme Review
• Directions: Using the diagram in your notes,
answer questions #1-#3.
Restriction Enzyme Review
Suspect #1 DNA
Restriction Enzymes are added
Suspect #2 DNA
Restriction Enzyme Review
Suspect #1 DNA
ATCGCCGGATCGCCGGATCGCCGGATCG
Suspect #2 DNA
ATCGATCGCCGGATCGATCGCCGGATCGATCG
Restriction Enzyme Review
Suspect #1 DNA
ATCGCC / GGATCGCC / GGATCGCC / GGATCG
Suspect #2 DNA
ATCGATCGCC / GGATCGATCGCC / GGATCGATCG
Think, Pair, Share
• Directions: Silently write a response to the
following question:
Why do we need restriction enzymes in gel
electrophoresis to help solve a crime?
Think, Pair, Share
• Directions: Discuss your answer within your
group.
Lab Agenda
Lab Agenda—Day 2
• Step #3: Load DNA samples from each suspect
into the gel
• Step #4: Run the gel by applying electricity
http://learn.genetics.utah.edu/content/labs/g
el/
Activity #1—Materials &
Lab Demonstration
What lab materials are needed for
Steps #3 and #4?
1. Agarose Gel
Agarose Gel (Gel)
Separates DNA fragments based on size
Activity #1—Materials &
Lab Demonstration
What lab materials are needed for
Steps #3 and #4?
1. Agarose Gel
2. Buffer
Buffer (Bf)
Carries electrical current
Activity #1—Materials &
Lab Demonstration
What lab materials are needed for
Steps #3 and #4?
1. Agarose Gel
2. Buffer
3. Gel Electrophoresis Chamber
Gel Electrophoresis Chamber
(GE Chamber)
Holds the gel
Activity #1—Materials &
Lab Demonstration
What lab materials are needed for
Steps #3 and #4?
1. Agarose Gel
2. Buffer
3. Gel Electrophoresis Chamber
4. Power source
Power Source (PS)
Provides electricity to pull DNA
fragments apart
Starting Your Lab Report on Google
Drive
1. Go to www.google.com/drive
2. Username: firstname.lastname@fhsaonline.org
3. Password: ID number
4. Click on the red “Create” button → Document
5. Name the document “Firstname.Lastname.GelLab”
6. Use 12 font
My Notes on the Procedure
• Directions: Watch as your teacher shows a
demonstration of the lab techniques used to
complete Steps #3 and #4.
DURING THIS TIME YOU ARE WRITING WHAT
YOU ARE OBSERVING IN AS MUCH DETAIL AS
POSSIBLE.
My Notes on the Procedure
While writing the procedure, try to answer
these questions:
What lab tools is my teacher using?
What ingredients are being mixed together?
What happens first, next, last?
Agenda
iLearnNYC Lesson 36 and 37
1. Quizlet, Les Animaux
2. Quizlet, Adjectives
3. Quizlet, Nouns
Finish Early? End-of-Week Assignment
Exit Ticket—due by the End of Class