Writing Laboratory Reports Delphine Masse, Wake Forest University Lab reports Objective: learn to report the results of a scientific inquiry in a generally used format Delphine Masse, Wake Forest University The parts of a lab report Title Abstract Introduction Methods Results Discussion Literature cited Tables and Figures Delphine Masse, Wake Forest University Title Describes your experiment Specific topic Mentions the organisms studied Delphine Masse, Wake Forest University Abstract Last thing to write! Usually < 200 words, summary of your whole experiment (ie: summarizes every part of the paper) – About one sentence per section Self explanatory & independent from paper No abbreviations or citations Delphine Masse, Wake Forest University Introduction Introduces the reader to your topic Explains why the topic is of interest Explains the main concepts necessary to understand your experiment States which organisms were used (with scientific name) Refers to literature States your purpose & hypothesis Explains how your experiment will address your purpose Delphine Masse, Wake Forest University Introduction : more specifically 1. 2. 3. 4. 5. General statement introducing the topic Why is this topic of interest ? Explain all concepts necessary to understand what you did. What is your purpose? Hypothesis 1. 2. 3. 6. 7. Includes the word “hypothesis” or “hypothesized” States your dependant and independent variables Gives rationale for your expectations How are you going to test this? How do the variables recorded reflect the phenomenon you want to measure? Briefly mention the outcome of the experiment Delphine Masse, Wake Forest University Hypothesis example eg: "Since male B. splendens have to protect fertilized eggs against predators, it was hypothesized that the aggressiveness of males, measured by the average number of displays per minute, would be higher in the presence of eggs than in the absence of eggs." Note: this assumes that you explained the link between displays and aggressiveness in the background Delphine Masse, Wake Forest University Methods Enough information so that the experiment can be repeated (But: assumes the reader has basic lab knowledge) Passive voice, past tense Contains: – Experimental setup: – Measurements taken: – Specific: Durations, Concentrations, Voltages, quantities Order in which things were performed Emphasize how the experimental groups differ from the controls. What did you record, how? In case you transformed data (eg. Used percentages…), why? Analysis Methods used: What type of statistics were used, why, and to compare what groups. Delphine Masse, Wake Forest University Methods - Examples: “0.5 mL of 10-6M Muristerone in water were added drop by drop to each of the four vials in group 2.” Delphine Masse, Wake Forest University Methods – examples: stats Not-so-good : "A t-test was calculated for the number of tail beatings” ambiguous! BETTER: "A student's t-test was used to compare the number of tail beatings in male B. splendens with the number of tail beatings in female B. splendens." Dependant variable (outcome) Test used First group of data points Second group of data points Delphine Masse, Wake Forest University Results Presents your results Past tense NO INTERPRETATION! Text: talks about trends & comparisons. Refers to figures &/or tables. Backs up statements with results from statistical analysis – include the p-values (that is what the text refers to as “state if your hypothesis was supported”) Delphine Masse, Wake Forest University Reporting results - Examples: The root:shoot ratio of plants grown in low-nutrient soil was significantly lower than the root:shoot ratio of the control (p = 0.03). The number of tail beatings in male B. splendens was significantly different from the number of tail beatings in females (p < 0.05). The root:shoot ratio of plants grown in low-nutrient soil was greater than, though not significantly different from, the root:shoot ratio of the control (p = 0.07). Delphine Masse, Wake Forest University Discussion Goal: Interpretation of data Addresses all the results presented before – and no new results (do not repeat the results) States your conclusions and your rationale (with your evidence + data from other research) Forms or refines hypotheses Puts your results into a larger context Delphine Masse, Wake Forest University Discussion – questions to cover 1) 2) Did the data correspond to what you expected? Why or why not? Did the data correspond to what other people found? (use citations here) 1) 2) 3) If no, why do you think it happened? Present a new hypothesis If yes, are there other factors that may have caused this outcome? Finish on further research / implications Delphine Masse, Wake Forest University Literature cited CBE format: see manual. 5 refs, incl. 3 journal articles, required. In the text: – – – Always paraphrase (no direct quotes) (Jones 2003) Eg: Methoprene has been shown to significantly affect the development of Drosophila melanogaster (Jones 2003). Or: Jones (2003) has found that …… Delphine Masse, Wake Forest University Literature cited (cont.) Some special cases: – Anonymous : Use a short version of the title – In text: (Lab. Manual 2003) Reference: _________. Spring 2004. Comparative physiology Laboratory Manual. Dept. Biology, Wake Forest University, Winston-Salem, NC. Paper you accessed online: Cite the print version NOT the database Delphine Masse, Wake Forest University Tables and Figures Present summary data (no raw data!) Tables then Figures Number tables and figures independently, in the order that they are mentioned in the text All tables and figures should be mentioned in the text Delphine Masse, Wake Forest University Tables - Example Table 1. P-values obtained when comparing Root:Shoot ratios of the different groups using a Student's t-test Delphine Masse, Wake Forest University Figures - Example Figure 1. Average resting blood pressure (mmHg) of a group of 15 undergraduates and a group of 15 graduate students. Error bars represent +/- SD for each group. Delphine Masse, Wake Forest University General format issues Numbers: – – If <1, always have a 0 in front : eg. 0.1 not .1 If <5 and no units then spell it out; >5 use number Eg: a group of four vials; a total of 12 vials Format: 1’’ margins, double spaced, 3 pages of text, 1 of figures (references can be on a separate page); Times 11 pt. Delphine Masse, Wake Forest University Extra requirements: Attach to the report a printout of – – – Your raw data, correctly arranged and labeled. The outputs for the statistical tests you did with Excel, correctly labeled. The first page of each scientific paper you cited. Do not mention these attachments in the text. These are just for verification purposes. Delphine Masse, Wake Forest University