Cell Cycle An Internet Activity Purpose: In this activity, you will be presented with cells from the tip of an onion root. You will classify each cell based on what phase it is in. Procedure: 1. Open Google Chrome. Go to http://www.biology.arizona.edu. 2. Click on the “Cell Biology” link about halfway down the page. 3. Under the “Activities” section in the upper left corner, click on the “On-line Onion Root Tips.” 4. Read the information and watch the animation. Answer the pre-lab questions on the worksheet. When finished, click “Next” at the bottom. 5. Scan the information about the 5 phases of the cell cycle. For this activity, Interphase will not be broken down and Cytokinesis will not be considered. Answer the pre-lab questions on the worksheet. When finished, click “Next.” 6. The table on the next page is the same as the table on the left page of your notebook. Click “Next.” 7. A cell will be presented to you. As a group, determine which phase you believe this is in. Click that phase. If you are correct, it will put that cell in the table. If you are not correct, it will describe the cell to you and tell you why you are wrong. Try again. 8. When you have classified all of the cells, count how many cells are in each phase of the table and put that number in your table on the left. They should equal 36 total cells. 9. Calculate the percentage of each phase by using the following formula: Number of Cells X 100 Total Number of Cells 10. Show your work in the calculations section. 11. Choose one cell from each column and draw it exactly as you see it in the space in the table. 12. Log out and close the computer unless the charge is less than 50%. If the charge is less than 50%, please show it to the instructor. 13. Answer the post-lab questions. Cell Cycle- Internet Activity Analysis Pre-Lab Background Information: a. Why are roots good for studying the cell cycle? _______________________________________________ _______________________________________________________________________________________ b. Although the root captures many cells in different phases of the cell cycle, what must we keep in mind? _______________________________________________________________________________________ _______________________________________________________________________________________ c. Next to each major event write phase of mitosis in which it occurs. Answers are used more than once. ___________________ Spindle fibers align the chromosomes along the middle of the cell nucleus ___________________ The DNA duplicates to prepare for mitosis ___________________ Cytokinesis may begin during this stage ___________________ Chromatin in the nucleus begins to condense ___________________ New membranes form around the daughter nuclei ___________________ Chromosomes are not clearly discerned in the nucleus ___________________ The paired chromosomes separate at the kinetochores and move to opposite sides of the cell ___________________ The nuclear membrane dissolves ___________________ The chromosomes disperse and are no longer visible under the light microscope Data Table: Interphase Number of Cells % of Cells Prophase Metaphase Anaphase Telophase Total 36 100% Drawing Calculations: Questions: 1) Which phase of the cell cycle did the cell spend the most time in? Why? ______________________________________________________________________________ ______________________________________________________________________________ 2) Describe what is happening in each phase in your table. ________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________