Title of Lesson: Plants—How Structure, Function, and Physiology Impact Their Role in the Carbon and Hydrologic Cycles Content/Level: Biology Grade 9 Lesson Overview: Students will investigate the following questions from labs in the text, Argument Driven Inquiry in Biology and work with supplementary texts and materials to develop an understanding of plant structure, the relationship between structure, function, and the properties of water in plants’ physiological processes (photosynthesis and transpiration), and how plants are players in the water and carbon cycles. Lab 5 Revised: Why Do Temperature and Light Intensity Affect the Rate of Photosynthesis in Plants ( Lab 7 Revised: Transpiration—How Do Environmental Factors (Light Intensity, Temperature, Wind, and Humidity) and Anatomical Features (Leaf Surface Area) Affect the Movement of Water Through a Plant Benchmarks: SC.912.L.14.2 Relate structure to function for the components of plant and animal cells. Explain the role of cell membranes as a highly selective barrier (passive and active transport). SC.912.L.14.7 Relate the structure of each of the major plant organs and tissues to physiological processes. SC.912.L.18.7 Identify the reactants, products, and basic functions of photosynthesis. SC.912.L.18.11 Explain the role of enzymes as catalysts that lower the activation energy of biochemical reactions. Identify factors, such as pH and temperature, and their effect on enzyme activity. SC.912.L.18.12 Discuss the special properties of water that contribute to Earth’s suitability as an environment for life: cohesive behavior, ability to moderate temperature, expansion upon freezing, and versatility as a solvent. SC.912.E.7.1 Analyze the movement of matter and energy through the different biogeochemical cycles, including water and carbon. SC.912.N.1.1 Define a problem based on a specific body of knowledge and do the following: 1. Pose questions about the natural world; 2. Conduct systematic observations 3. Examine books and other sources of information to see what is already known 4. Review what is known in light of empirical evidence 5. Plan investigations 6. Use tools to gather, analyze, and interpret data (this includes the use of measurement in metric and other systems, and also the generation and interpretation of graphical representations of data, including data tables and graphs) 7. Pose answers, explanations, or descriptions of events 8. Generate explanations that explicate or describe natural phenomena (inferences) 9. Use appropriate evidence and reasoning to justify these explanations to others 10. Communicate results of scientific investigations 11. Evaluate the merits of the explanations produced by others Relevant Achievement Level Descriptions Compare structures and describe related functions in different types of cells Relate structures of plant tissues and organs directly to their roles in physiological processes (photosynthesis and transpiration) Analyze how enzymes speed up the rate of a biochemical reaction and describe the effect of environmental factors on enzyme activity Summarize the properties of water and analyze how these properties make water essential for life on Earth. Analyze the movement of matter through different biogeochemical cycles (water cycle and carbon cycle) Interpret, analyze, and synthesize data to determine causal relationships in a complex investigation Evaluate the reliability of other sources of information to make predictions and defend conclusions based on experimental design or scientific argumentation Students should be able to: Know Understand Do Plants are autotrophic Plants undergo the chemical Photosynthesis ADI—How (produce their own food) reaction photosynthesis to and why do temperature and produce their own food. light intensity impact the rate Photosynthesis rely on carbon of photosynthesis dioxide and water as reactants, therefore Factors That Affect photosynthesis is a player in Photosynthesis Informational both the carbon and water Text cycle. Photosynthesis Lab CEJ Template & Reflective Questions Plants utilize only about 1% of Transpiration is an important Transpiration ADI— How Do the water taken in at the roots player in the hydrologic Environmental Factors (Light for photosynthesis. The other (water) cycle, which returns Intensity, Temperature, Wind, 99% evaporates at the leaf water from the soil to the and Humidity) and surface in a process called atmosphere. Anatomical Features (Leaf transpiration. Surface Area) Affect the Transpiration is impacted by Movement of Water Through both environmental factors a Plant and the plant’s anatomical features. Transpiration Lab CEJ Worksheet Transpiration Assessment Plants have specialized cells, Students should understand Plant Structure and Function tissues, and organs that assist the structure and function of in Photosynthesis and in the processes of plant organs, including: roots, Transpiration Worksheet photosynthesis and stems, and leaves. transpiration. Students should understand the structure and function of plant tissues including: dermal, ground, and vascular. Students should be able to cite examples of each tissue type in the roots, stems, and leaves. Students should identify the types of cells found in plant tissues, including: parenchyma, sclerenchyma, and collenchyma. Students should be able to cite examples of each cell type in the roots, stems, and leaves. Transport of Matter in Plants Writing Prompt Students should relate plant structure and function to transpiration and photosynthesis, and hence the hydrologic and carbon cycles. Water processes (osmosis, Students should relate these Celery Model of Water transpiration) and properties processes and properties to Transport & Extension CEJ (solvent, surface, tension, movement of water through cohesion, adhesion) xylem in a plant, which is contribute to movement of required for photosynthesis water through a plant. and transpiration. Text Reference(s): Factors That Affect the Rate of Photosynthesis-- Adapted from: http://www.passmyexams.co.uk/GCSE/biology/factors-affecting-rate-ofphotosynthesis.html How Do Large Trees, Such As Redwoods, Get Water From Their Roots to The Leaves? http://www.scientificamerican.com/article/how-do-large-trees-such-a/ The Simple Story of Photosynthesis and Food Electronic http://ed.ted.com/lessons/the-simple-but-fascinating-story-of-photosynthesis- References and-food-amanda-ooten The Happy Scientist: Heartless Plants Username: hillsboroughmiddle Password: hillsboroughmiddle http://thehappyscientist.com/science-video/heartless-plants Plant Transpiration Lab https://www.classzone.com/books/hs/ca/sc/bio_07/virtual_labs/virtualLabs.html Water Transport in Plants Video Clips a. Plant Transport, Xylem & Phloem—Transpiration (00:05:37) https://www.youtube.com/watch?v=xGCnuXxbZGk b. Water Transport in Plants (00:03:18) http://tstem.psjaisd.us/apps/video/watch.jsp?v=41282 Electronic Attachments Day 1 -2 Photosynthesis Lab Gizmo Data Set Revised ADI Lab 5 Photosynthesis—How and Why Do Temperature and Light Intensity Affect the Rate of Photosynthesis in Plants? Photosynthesis Lab Paper Whiteboard Template Factors That Affect the Rate of Photosynthesis Text CEJ Photosynthesis Lab Template & Reflective Questions Revised ADI Lab 7—How Do Environmental Factors and Anatomical Features Affect the Rate of Transpiration in Plants? Revised ADI Request for Proposal 3 Transpiration Paper Whiteboard Template CEJ Transpiration Lab Worksheet Transpiration Assessment Plant Structure and Function in Photosynthesis and Transpiration Worksheet Celery Model of Water Transport Does Adding Solute Affect the Rate Water is Transported Through the Xylem—Paper Whiteboard Template Transport of Matter in Plants Writing Prompt Objective Students will complete and ADI and analyze a data set (from the Gizmo, “Photosynthesis Lab” to determine how temperature and light intensity affect the rate of photosynthesis in plants. Materials Pacing Computer with Internet Connection & LCD Projector Photosynthesis Lab Gizmo Data Set ADI Lab 5: Photosynthesis- How and Why Do Temperature and Light Intensity Affect the Rate of Photosynthesis in Plants? Photosynthesis Lab Paper Whiteboard Template One class period (approximately 50 minutes) Advanced Prep: Print color copies of the data set from the Gizmo, “Photosynthesis Lab” for each group of 3-4 students. Xerox copies of (a) the ADI Lab 5, “Photosynthesis--Why Do Temperatures and Light Intensity Affect the Rate of Photosynthesis in Plants” and (b) Paper Whiteboard Template for each student. ENGAGE: The Simple Story of Photosynthesis and Food http://ed.ted.com/lessons/the-simple-but-fascinating-story-of-photosynthesis-and-food-amandaooten EXPLORE: (50 minutes) 1. Provide each student with a copy of (a) the ADI, “Photosynthesis: How and Why Do Temperature and Light Intensity Affect the Rate of Photosynthesis?” and (b) a copy of a paper whiteboard. This ADI includes some background information, including a basic review of photosynthesis, as well as prompting students that photosynthesis does not occur all of the time, and that certain environmental factors impact the rate it occurs. 2. After reviewing the ADI with students, provide each group (3-4 students) with a Photosynthesis Lab Gizmo Data Set. The data set includes two sets of graphics showing (a) the relationship between light intensity and oxygen production and (b) temperature and oxygen production. 3. Student groups should analyze the data set and construct a paper whiteboard, indicating their claim, evidence, and justification on the template provided. Instruct students that they will need this completed paper template the following day in class when they construct their actual whiteboard in preparation for carousel review. (Note: The purpose of constructing a paper whiteboard is that we have found there is not enough instructional time in a 50-minute class period to analyze the data, construct a whiteboard, and complete a sufficient carousel review and discussion.) Day 2 Objective Students will communicate their claim, evidence, and justification to other student researchers using a carousel review. Materials Pacing Completed Photosynthesis Lab Paper Whiteboard Template (from Day 1) ADI Whiteboards Portable Tripods (to display whiteboards) Dry Erase Markers Erasers One class period (approximately 50 minutes) Advanced Prep: Ensure you have a whiteboard set (whiteboard, tripod, dry erase markers, and eraser) for each group of 3-4 students. ELABORATE/EXTEND: (50 minutes) 1. Have students transfer the information on their paper whiteboard to their actual whiteboard. Students will take forever embellishing, so we recommend that this be a timed activity. (15 minutes) 2. Explain the carousel review process to students, indicating each round will last five minutes. See diagram below. (5 minutes) 3. Conduct a minimum of four rounds of carousel review to allow students to view multiple perspectives. (20 minutes) 4. Allow students to reconvene with their home group to discuss what they learned from other groups and to consider possible changes to their paper whiteboards. (10 minutes) Day 3 Objective Students will participate in a class discussion and read a piece of informational text to make additions/revisions to their paper whiteboards. (In particular, we hope that students will make significant adjustments to their scientific justification of their evidence.) Materials Pacing Supplemental Text: “Factors That Affect the Rate of Photosynthesis” Completed Photosynthesis Lab Paper Whiteboard Templates (From Day 1) One class period (approximately 50 minutes) Advanced Prep: Xerox a copy of the supplemental text, “Factors That Affect the Rate of Photosynthesis” for each student. You might wish to Xerox additional copies of the Photosynthesis Lab Paper Whiteboard Templates to have on hand for students who wish to make significant revisions. EXPLAIN: (50 minutes) 1. Conduct a whole class discussion, asking questions to promote thinking, such as (10 minutes) (a) What did you learn from other groups that made you skeptical? (b) What did you learn from other groups that corroborated your findings? (c) What other information would you like to have, or what other experiments would you propose? (d) What did you expect and what surprised you as you analyzed the data sets? Why? (e) Why do you think the rate of photosynthesis changed when light intensity changed? Temperature changed? What “science” might explain this? 2. Provide students with the supplemental informational text, “Factors That Affect the Rate of Photosynthesis.” Have them read for potential lines of justification to explain the trends observed in the data sets in order to explain their claim and evidence. (We would expect that most student groups produced weak justification statements, or may have missed the mark entirely—and that is okay. This piece of text should help them make sense of their evidence.) (15 minutes) 3. Prompt student groups to collaborate with their group to amend their paper whiteboard templates to reflect new learning gleaned from the carousel review, whole class discussion, and the supplemental informational text. You may wish to have blank copies on hand. (25 minute) Day 4 4& Objective Students will complete CEJ Photosynthesis Lab & Reflective Questions as summative measure of their learning. Materials Pacing CEJ Photosynthesis Lab Template & Reflective Questions ADI Lab 5: Photosynthesis- How and Why Do Temperature and Light Intensity Affect the Rate of Photosynthesis in Plants? (Student Optional) Photosynthesis Lab Paper Whiteboard Template (Student Optional) Photosynthesis Lab Gizmo Data Set (Student Optional) One class period (approximately 50 minutes) Advanced Prep: Xerox a copy of CEJ Photosynthesis Lab & Reflective Questions for each student. EVALUATE (50 minutes) 1. Provide each student with a copy of CEJ Photosynthesis Lab & Reflective Questions to complete independently as a means of summative assessment. Students may refer to their copy of the ADI, the data set, and their paper whiteboard in completion of this assessment. (50 minutes) Day 5 Objective Students will complete an investigation proposal to determine whether leaf surface area and other environmental factors impact the rate of transpiration in plants. Materials Pacing Computer with internet connection & LCD Projector Revised ADI Lab 7: How do environmental factors (light intensity, temperature, wind, and humidity) and anatomical features (leaf surface area) affect the rate of transpiration in plants? Revised ADI Request for Proposal 3 One class period (approximately 50 minutes) Advanced Prep: Ensure you have an internet connection and log on to the Happy Scientist to show the “Heartless Plants” video clip. Xerox copies of the revised ADI Lab 7: How do environmental factors (light intensity, temperature, wind, and humidity) and anatomical features (leaf surface area) affect the rate of transpiration in plants? for each student. Xerox copies of the Revised ADI Investigation Proposal C for each student. ENGAGE: (10 minutes) 1. Show the video clip, “Heartless Plants” from the Happy Scientist (3 minutes) http://thehappyscientist.com/science-video/heartless-plants Username: hillsboroughmiddle Password: hillsboroughmiddle 2. Discuss some of the major ideas in the video, which might include (5 minutes) (a) Photosynthesis occurs in leaves, and one of the reactants is water. (b) Water must be able to get from the roots to the leaves in plants. (c) The xylem is the plant tissue responsible for transporting water from the roots to the leaves. (d) When water vapor is released from the plant during transpiration, this creates negative pressure in the xylem that draws water from the roots to the leaves, similar to how liquid moves through a drinking straw. 3. Tell students we will investigate environmental and anatomical features that impact the rate of transpiration in plants. (2 minutes) EXPLORE: (40 minutes) 1. Provide each student with a copy of the revised ADI Lab 7: How do environmental factors (light intensity, temperature, wind, and humidity) and anatomical features (leaf surface area) affect the rate of transpiration in plants? 2. Review the lab with students and determine a mechanism for assigning lab groups a variable to test (light intensity, temperature, wind, humidity, or leaf surface area). 3. Provide each student with a copy of the revised ADI Investigation Proposal C. With their lab group, they should complete the investigation proposal and get it approved by the teacher. Day 6 Objective Materials Students will investigate a variable that impacts the rate of transpiration in plants. Revised ADI Lab 7: How do environmental factors (light intensity, temperature, wind, and humidity) and anatomical features (leaf surface area) affect the rate of transpiration in plants? (From Day 5) Transpiration Paper Whiteboard Template Lab materials for ADI Lab 7 (See list below): Calibrated/Graduated Pipettes Plastic Tubing Parafilm or Vaseline Ring Stands Clamps Pacing Plant Samples Water Lamps Various Wattage Light Bulbs Desk Fan Large Ziplock Bags Twist Ties or Rubber bands Plastic Tubs Various Lab Glassware Scissors Ruler Centimeter (cm2) Grid Graph Paper Microscope Slides Clear Packing Tape Electronic Balance One class period (approximately 50 minutes) Advanced Prep: Obtain enough plants for each student group to have a stem with healthy leaves. (These can be woody plants, shrubs, bean sprout seedlings, etc. Be certain to follow the directions for cutting the stems in the lab procedure) Each group should experiment with the same species to eliminate this an additional variable in experimentation. Ensure you have compiled the materials for ADI Lab 7--How do environmental factors (light intensity, temperature, wind, and humidity) and anatomical features (leaf surface area) affect the rate of transpiration in plants? Familiarize yourself with the techniques for building a potomter, measuring leaf surface area, and making stomatal leaf impressions so you can support students. Xerox a copy of the Transpiration Lab Paper Whiteboard Template for each student. EXPLORE: (50 minutes) 1. Student groups should perform their experiment, collect, and analyze their data according to their approved, “Revised ADI Investigation Proposal C.” 2. Students should review the directions for the revised Presentation Session on the ADI Lab 7-How do environmental factors (light intensity, temperature, wind, and humidity) and anatomical features (leaf surface area) affect the rate of transpiration in plants? and complete their own Transpiration Lab Paper Whiteboard Template for homework. (Homework) Note: There is a good digital transpiration lab at the following web address that you might have students complete as a supplemental activity for the variables they did not test directly. https://www.classzone.com/books/hs/ca/sc/bio_07/virtual_labs/virtualLabs.html Day 7 Objective Students will communicate their claim, evidence, and justification to other student researchers in a group presentation to the scientific community (classmates & teacher). Materials Pacing Completed Transpiration Lab Paper Whiteboard Template (from Day 6 Homework) ADI Whiteboards Portable Tripods (to display whiteboards) Dry Erase Markers Erasers CEJ Transpiration Lab Worksheet (optional use during presentation session) One class period (approximately 50 minutes) Advanced Prep: Ensure you have a whiteboard set (whiteboard, tripod, dry erase markers, and eraser) for each group of 3-4 students. Xerox copies of CEJ Transpiration Lab Worksheet if you would like to hold students accountable for the presentations of other groups during the presentation session. ELABORATE/EXTEND: (50 minutes) 1. Have students transfer the information on their paper whiteboard to their actual whiteboard. Students will take forever embellishing, so we recommend that this be a timed activity. Students also should determine the responsibilities of each group member during their presentation. (15 minutes) 2. Use a random selector to determine the order in which each group will present. Allow groups up to 5 minutes to present their findings. You may wish to have students complete the CEJ Transpiration Lab Worksheet as they listen to group presentations. (35 minutes). Day 8 Objective Students will complete questions 1-3 on the Transpiration Assessment as a summative measure of their learning. Students will research how plant anatomy is related to the processes of photosynthesis and transpiration. Materials Transpiration Assessment Plant Structure & Function in Photosynthesis and Transpiration Pacing One class period (approximately 50 minutes) Advanced Prep: Xerox a copy of the Transpiration Assessment for each student. Xerox a copy of Plant Structure & Function in Photosynthesis and Transpiration for each student. ELABORATE/EXTEND: (25 minutes) 1. Provide each student with a copy of Transpiration Assessment to complete independently as a means of summative assessment. Students may refer to their copy of the ADI, their CEJ notes from the Presentation Session, and their own paper whiteboard in completion of this assessment. (30 minutes) EXPLAIN: (25 minutes) 2. Provide each student with a copy of Plant Structure & Function in Photosynthesis and Transpiration. After the assessment, students should use their biology textbook to research plant structures in the roots, stems, and leaves and relate them to the processes of photosynthesis and transpiration. Students should complete this assignment for homework. Day 9 Objective Students will learn about properties of water through a lab investigation, “Celery Model for Water Transport.” Materials Lab Materials for Celery Model for Water Transport Various solutes (salt, sucrose) Various solvents (water, rubbing alcohol) Cups or Beakers Celery (lots of it) Pennies Capillary tubes Straws Petri dishes Pipettes Glass microscope slides Food coloring Graduated cylinders Various laboratory glassware Computer, internet connection, and LCD projector Pacing Celery Model for Water Transport Lab Celery Model for Water Transport CEJ Whiteboard Paper Template One class period (approximately 50 minutes) Advanced Prep: Xerox a copy of the Celery Model for Water Transport for each student. Gather materials and set up the Celery Model for Water Transport lab. ENGAGE (Approximately 5 minutes) 1. Preview the video clips below, and select the one that you believe best meets the needs of your students. Each covers the movement of water in vascular plants. c. Plant Transport, Xylem & Phloem—Transpiration (00:05:37) https://www.youtube.com/watch?v=xGCnuXxbZGk d. Water Transport in Plants (00:03:18) http://tstem.psjaisd.us/apps/video/watch.jsp?v=41282 EXPLORE (45 minutes) 1. Students should set up the Celery Model for Water Transport lab and read the background information in the lab handout. Day 10 Objective Students will learn about properties of water through a lab investigation, “Celery Model for Water Transport.” Students will communicate their claim, evidence, and justification to other student researchers in a group presentation to the scientific community (classmates & teacher). Materials Celery Model for Water Transport Paper Whiteboard Template Pacing One class period (approximately 50 minutes) Advanced Prep: Xerox a copy of Celery Model for Water Transport CEJ Paper Template for each student. EXPLORE (Approximately 5 minutes) 1. Students will complete the Celery Model for Water Transport Lab a) Find final mass b) Calculate change in mass 2. Students will complete the Celery Model for Water Transport Paper Whiteboard Template and complete for homework if necessary. Day 11 Objective Students will communicate their claim, evidence, and justification to other student researchers in a group presentation to the scientific community. (classmates & teacher). Materials Pacing Completed Celery Model for Water Transport Lab Paper Whiteboard Template ADI Whiteboards Portable Tripods (to display whiteboards) Dry Erase Markers Erasers CEJ Celery Model for Water Transport Lab Worksheet (optional use during presentation session) One class period (approximately 50 minutes) Advanced Prep: Ensure you have a whiteboard set (whiteboard, tripod, dry erase markers, and eraser) for each group of 3-4 students. Xerox copies of CEJ Celery Model for Water Transport Paper Whiteboard Template if you would like to hold students accountable for the presentations of other groups during the presentation session. ELABORATE/EXTEND: (50 minutes) 1. Have students transfer the information on their paper whiteboard to their actual whiteboard. Students will take forever embellishing, so we recommend that this be a timed activity. Students also should determine the responsibilities of each group member during their presentation. (15 minutes) 2. Use a random selector to determine the order in which each group will present. Allow groups up to 5 minutes to present their findings. You may wish to have students complete the CEJ Celery Model for Water Transport Lab Worksheet as they listen to group presentations. (35 minutes). Day 12 Objective Students will compose a written response to a writing prompt as a summative measure of their learning. Materials Pacing One class period (approximately 50 minutes) Transport of Matter in Plants Writing Prompt Prior assignments and biology textbook may be used. Ensure you have a whiteboard set (whiteboard, tripod, dry erase markers, and eraser) for each group of 3-4 students. Xerox copies of CEJ Transpiration Lab Worksheet if you would like to hold students accountable for the presentations of other groups during the presentation session. Lesson Written By: Mindy Pearson OTG Science Coach HCPS Secondary Education