INTEGRATED SCIENCE
DAY 5
September 14, 2012
LEARNING TARGETS
1.
2.
I can write a scientifically based conclusion
I can perform an experiment on a living
organism to test the effects of different
chemicals.
AGENDA
1.
2.
3.
4.
5.
6.
Kick off question: Look at your scored heart
rate lab –what areas could you improve on
Entry task
Conclusion writing and homework
instructions
Daphnia Lab Instructions
Daphnia Lab
Homework: Conclusion for heart rate lab on
separate sheet of paper in paragraph style
ENTRY TASK-SEPTEMBER 14
Look at your returned Heart rate, correct anything
I wrote comments on. Remind yourself what the
purpose of the experiment was. Listen as I
describe how to write a good conclusion. Now
write the answers to the conclusion questions in
complete sentences.
HOW TO WRITE A CONCLUSION:
You asked a question, you did an experiment, and you did the
experiment a second time. You recorded your results. Now it is
time to write your conclusion.
The conclusion, plain and simple, is the answer to your question.
It should be clear, concise and stick to the point. Resist the
temptation to jump to conclusions.
 If you were to do your experiment again, would you get the same
results?
 Can there be differences? Why?
 Ask yourself what happened when you tested your hypothesis.
 What have you learned from this experiment? What does this
experiment tell us
Now for homework-- using the answers to the questions on the
heart rate lab, write a final report summarizing your question,
research methods and conclusion.

Work on your conclusion on a separate piece of paper
until lunch. If you complete the conclusion, staple it to
your lab report and turn it into the second period box.
AFTER LUNCH
Read together the introduction to the daphnia lab
 Read the purpose
 Your table partner will be your lab partner, you
will be in teams of two instead of three. Make
sure that everyone has at least one partner.
 Each team will receive an alcohol fact sheet, a
caffeine fact sheet and a nicotine fact sheet
 Read through the fact sheets and record the
important information from each sheet on to the
data table for your lab.

REVIEW OF MICROSCOPE
Use your assigned microscope and return it to its
proper place as instructed
 Always start on low power
 Make sure your lenses are clean
 We will be using depression lenses, make sure
your Daphnia is in the well and do not overflow.

BIOASSAYS

Dose/Response Bioassays are used to:
Estimate toxicity to humans.
 Estimate maximum concentrations of specific
chemicals allowed to discharge into bodies of water.
 Investigate hazardous waste sites.

BIOASSAY SPECIES

3 organisms are often used
Daphnia
 Duckweed
 Lettuce Seeds


Expose organisms to chemicals and measure how
they respond.
9
REMEMBER YOU ARE WORKING WITH
A LIVING CREATURE


http://www.microscopyu.com/moviegallery/pondsc
um/daphnia/
At the end of each experiment return your
daphnia to the Rehabilitation and Recovery area
on the demonstration desk. Use a new daphnia
for each chemical
DAPHNIA
PART 1
Take a look at your daphnia on low power-use the
sketch I put on the overhead to help sketch and
label your daphnia
 Look at your daphnia on medium power and
sketch and label what you see
 Do not use high power with daphia or well plates

PART 2
Make sure to get a new daphnia for each
chemical you try
 You will need a timer to do the heart rate
 Make sure to record beats/min
 Return daphnia to the rehab bowl
 Clean up your lab station and return your
microscope to the cabinet as directed

DATA
Make sure to put your averages into the data
table at my desk
 Make sure to write down the class average
 Graph your data using a bar chart, showing your
averages and the class averages

CONCLUSION
If time permits fill in the conclusion parts of the
lab. Next class we will use this information and
some background information about the drugs to
build a more complete conclusion
 Turn in your lab report whether you complete the
report or not.
