Is the Bacteria Living in Our Environment Harmful To us? Disease Ecology: Bacteria A presentation created by: Tamanisha John & May Choi What is Disease Ecology? Ticks are skin parasites. They like motion, warm temperatures, and carbon dioxide exhaled by mammals. Disease Ecology is the study on how diseases spread through and impact host populations, and how hosts, pathogens, and their environment react and evolve in response to one another. I. What are Bacteria? Bacteria are living singlecelled organisms who are neither plants nor animals. Instead, they belong to their own group. Living Organisms Below: Plants Bacteria Animals single-celled microorganisms appeared on earth about 4 billion years ago. Scientists say they were the first life forms on Earth. II. What Are Bacteria? Bacteria come in three main shapes: Spherical (like a ball) Rod Shaped Spiral Spherical are usually referred to as cocci Rod Shaped are usually referred to bacilli Spiral are usually referred to as spirillia Did you know that Planet Earth is estimated to hold at least 5 nonillion bacteria! (Nonillion in U.S. means there are 30 zero’s after a number, while in the U.K there are 54 zero’s after the number. We’re using the U.S. version) (medicalnewstoday.com) Bacteria: The Good, The Bad, & The Ugly Why do you think we used tooth pictures? The Good: The Bad & The Ugly: Without bacteria we would die. The good bacteria help us digest our food and they live on our skin and in our mouth to provide protection against bad bacteria. Simply put, can either make us sick or dead. Agar V.S. Gelatin Jell-O Why Can Gelatin Substitute Agar? Gelatin can substitute agar because they are both gelling agents. Agar, however, is more preferable due to its long term solidity, meaning that unlike gelatin, which is liquefied easier, agar will stay solid. Think of Gelatin as ice, it won’t stay solid forever. The Aim Of Our Experiment: Is to figure out whether or not the bacteria in our school have a negative or positive impact on our environment. To decide whether or not they are harmful, we plan to harvest the bacteria we collect and place it on an apple slice in order to see whether the apple slices rot or turn bad quickly. If the bacteria have a negative affect on the apple slices, it will probably be a bad thing for our environment. Our Hypothesis Since bacteria can only be considered very dangerous in large amounts, but even then, small amounts of bacteria can reproduce rapidly, we do not think that the bacteria in our school is any more harmful than the common cold because we all act and interact with our environment daily and nothing too serious (like someone dying) happens. Do you think the bacteria in our school is dangerous? Materials: Gelatin (Jell-O), Microscope, Beaker with Water, 3 Q-tips, 3 Petri Dishes, 3 Zip Lock Baggies, Marker, Knife, Gloves, Tape, Bleach, Apple Before Starting Our Experiment: we collected bacteria from a toilet handle in the girls bathroom, one of the computer keyboards in Meghan’s office, and one of the auditorium chairs in the auditorium. Then we swiped the collected bacteria in our Gelatin, secured our collected data and went on winter break The Experiment Safety Steps to Consider: 1.Wear Gloves when handling bacteria 2. Wash Hands Frequently 3. Bleach bacteria at the end of experiment What Do They All Have In Common? Mold! Mold is a type of fungus (an organism that lacks chlorophyll and feeds on organic matter) and some funguses are pathogenic. Mold travels through the air in the form of tiny spores (not visible to the naked eye) and like to make their way to damp and moist areas so that they can breed and multiply. Data and Results: 25 20 Control 15 Girls Bathroom 10 M e ghan's Office Auditorium 5 0 1/5/2011 1/6/2011 1/7/2011 All of the apple slices weight gradually decreased as they started to rot. However, our control’s apple slice weighed more than any of the other apple slices indicating it wasn’t rotting as quickly. We noticed that our control’s apple slice will decreased by about 2grams while all the other slices decreased by about 3grams. I. Observations On the second day of our experiment (1/6/2011), we observed that our control apple slice and the one with bacteria from a keyboard in Meghan’s office were soft around their edges, but hard on their backs and middle slice while the other two were softer. II. Observations On the second day we also noticed that our mold turned grey, which, according to gardenline.usask.ca , means that our mold must’ve gotten cooler and wetter than before, which did happen since each day our Gelatin got more liquefied. & Our Hypothesis Was… Partially Correct! During the course of our experiment, we found the bacteria from the toilet handle in the girl’s bathroom can be considered dangerous. Looks like my job won’t be over anytime soon Thinking Outside The Box To figure out why the girls bathroom bacteria rotted its apple faster than any other bacteria, besides the fact that it must’ve multiplied faster, we ran a survey in our school surveying 25 girls (both teachers and students), in order to figure out how they flush the toilet. Out of the 25 girls surveyed, 20 flushed the toilet with their feet, while 5 girls flushed the toilet with their hands. Sources Of Error •Other occurring experiments that could’ve added carbon to the air (i.e. the experiments with fire) •Natural air borne bacteria which could have had an effect on the rotting processes of our apples, since they could have been attracted to the bacteria we collected and put on them and speeded up the rotting process •Survey’s small sample size Future Experiment: Only collect bacteria from the girl’s bathroom toilet handle, but a week in advance, “monitor” or put up a sign in the girl’s bathroom saying ‘Do Not Flush Toilet With Your Shoes, Experimental Reasons,’ in order to collect more accurate data without the possibility of shoe germs. Why Would You Do That? While conducting our survey, the usual response was: “Ew, who touches that dirty handle?” If every girl assumes the handle is dirty, then there’s less of a chance that any of them would want to touch the handle, therefore, they will use their shoe and shoe germs will get on the handle. Bibliography Trudy Wassenaar. Bacteria: Good or Bad?. www.newton.dep.anl.gov. November 10, 1991. Newton Ask A Scientist. December 23, 2010 Science Clarified. Parasites. http://www.scienceclarified.com/OiPh/Parasites.html. 2011. Advameg, Inc. December 17, 2010 Trudy Wassenaar. What Are Bacteria. www.bacteriamuseum.org. 1998. The Virtual Museum of Bacteria. Educational Resources. December 24, 2010 Nordqvist, Christian. What is Bacteria? What are Bacteria?. http://www.medicalnewstoday.com/articles/157973.php. July 17, 2009. Medical News Today. December 30, 2010 The University of Georgia: Biomedical & Health Science Institute. Ecology of Infectious Disease a Research Initiative at the University of Georgia. www.biomed.uga.edu. September 7, 2010. University of Georgia BHSL. December 24, 2010 Centers for Disease Control and Prevention. Molds in the Environment. http://www.cdc.gov/mold/faqs.htm. February 8, 2010. National Center for Environmental Health. January 10, 2011 George A. Miller, Christiane Fellbaum, Randee Tengi, Helen Langone, Adam Ernst, Lavanya Jose. WordNet a Lexical Datatbase for English. www.wordnetweb.princeton.edu. 1995. MIT Press. December 23-30, 2010 S.E. Smith. What is Agar?. www.wisegeek.com. 2003. Conjecture Corporation. December 23, 2010 Steve Spangler. Growing Bacteria in Petri Dishes. www.stevespanglerscience.com. 2009. Steve Spangler Science. December 24, 2010 Nordqvist, Christian. What is Fungus? What are Fungi?. http://www.medicalnewstoday.com/articles/158134.php. July 21, 2009. Medical News Today. January 10, 2011 Kilpatrick, A.M. & Altizer, S. (2010) Disease Ecology. Nature Education Knowledge 1(12):13. December 20, 2010 Spangler, Steve. Growing Bacteria in Petri Dishes. www.stevespanglerscience.com. 2009. Steve Spangler Science. December 31, 2010 Shannan Muskopf. Estimating Population Size. www.biologycorner.com. 2009. The Biology Corner Wordpress. January 1, 2011 Smith, S.E. What is Agar?. www.wisegeek.com. 2003. Conjecture Corporation. December 30, 2010 University of Saskatchewan Extension Division: Department of Plant Sciences and Provincial Government. Gardenline. www.gardenline.usask.ca. 1994. U of S. January 4, 2011 Anglin, M.R. What are Pathogens?. http://www.wisegeek.com/whatare-pathogens.htm. December 2, 2010. Conjecture Corporation. December 28, 2010