Genetics Mendelian Genetics While assigned to teach, he was also assigned to tend the gardens and grow vegetables for the monks to eat. Augustinian Monk at Brno Monastery in Austria (now Czech Republic) Not a great teacher but well trained in math, statistics, probability, physics, and interested in plants and heredity. Gregor Mendel “Father of Genetics” Mountains with short, cool growing season meant pea (Pisum sativum) was an ideal crop plant. Gregor Mendel’s Work • Starting in 1856 Mendel studied peas which he grew in a garden out side the Abbey he lived in. • Showed that the traits he studied behaved in a precise mathematical way and disproved the theory of "blended inheritance.” • Mendel’s work was rediscovered in 1900 by three botanists: – Carl Correns (Germany) – Erich von Tschermak (Austria) – Hugo de Vries (Holland) Why Peas? Mendel used peas to study inheritance because: – True breeding commercial strains were available – Peas are easy to grow – Peas have many easy to observe traits including: Consistency is Good Gene Expression • • Each form of the particular gene is an allele. Alleles can be either 1. Dominant – always show trait - T 2. Recessive – only see if dominant trait absent – t • In order to see the trait expressed, 2 alleles must be paired together (one from mom and one from dad) T + t Tt Gene Expression • Genes come in pairs that separate during the formation of gametes (meiosis). • The members of these pairs can be the same (homozygous) or different (heterozygous). Practice: Take out the Applying Genetics Terminology paper and complete. You have 2 minutes. Gene Expression • When two alleles are paired we can express them by their phenotype or genotype. – Phenotype – physical characteristics • Ex. Tall, Short, Dark, Round, Wrinkled – Genotype – the allele combination or genes • Ex. AA, Aa, aa, Dd, Rr, rr, tt Using terminology: Results of Mendel’s monohybrid parental cross: “Mendel’s Principle of Dominance” F1 offspring of a monohybrid cross of true-breeding strains resemble only one of the parents. Why? Smooth seeds (allele S) are completely dominant to wrinkled seeds (allele s). Example of Mendel’s Work P Smooth SS x Wrinkled ss Homozygous Dominant Homozygous Recessive All Smooth Ss F1 Heterozygous Clearly Smooth is Inherited… What happened to wrinkled? 1. Smooth is dominant to wrinkled 2. Use S/s rather than W/w for symbolic logic F1 x F1 = F2 Punnett Square: F2 possible gametes Phenotype Genotype possible gametes S s Smooth Smooth S SS Ss s Smooth wrinkled Ss ss NEVER use S/W or s/w 3/ Smooth 1/ wrinkled 4 4 wrinkled is not missing…just masked as “recessive” in diploid state Mendel as a Scientist Test Cross: Unknown Smooth x possible gametes If Unknown is Ss: Punnett Square: Wrinkled ss possible gametes If Unknown is SS: s s possible gametes possible gametes S s S Smooth Smooth SS Ss s Smooth wrinkled Ss ss s S Smooth Smooth Ss Ss S Smooth Smooth Ss Ss Test Progeny All Smooth possible gametes s S Smooth Ss possible gametes F1 x F1 = F2 F2 Wrinkled ss s Smooth Ss Wrinkled ss Test Progeny Half Smooth Half wrinkled “Mendel’s Principle of Segregation”: • Recessive characters masked in the F1 progeny of two true-breeding strains, reappear in a specific proportion of the F2 progeny. • Two members of a gene pair segregate (separate) from each other during the formation of gametes. Segregation Monohybrid Crosses Yielded Consistent Results Therefore, the Principle of Segregation indeed is a general principle of genetics. Probability and Genetics Equation for probability NUMBER OF THINGS YOU ARE LOOKING FOR PROBABILITY = ----------------------------------TOTAL NUMBER OF THINGS I have quarter in my pocket. What is the probability that I get heads when flipped? Answer: ½ You have a total of 2 sides and 1 of them is heads. I have 3 pennies and 5 nickels in my pocket. If I pull out one coin what is the probability that I get a nickel? Answer: 5/8 You have a total of 8 coins and 5 of them are nickels. If I tossed a coin 100 times, how many heads would you expect to get? Tails? 50 each What if you didn’t get 50: 50? How would you know if the numbers you got were good enough? Chi-Square Analysis – determine how close your data is to the known probability of occurrence Chi-Square Lets look at the chance of flipping heads or tails Options Heads Tails Observed (o) 43 57 Expected (e) 50 50 N (degree of freedom) = # of options – 1 N=2-1 N=1 o – e (d) d2 d2/e 43-50 = 72 = 49/50 = -7 49 .98 57-50 = 72 = 49/50 = 7 49 .98 X2 = 1.96 Add this column for X2 Chi-Square cont. N=1 X2 = 1.96 What do I do with these numbers? Once the Chi-square and N values are computed, look on the chart. Degrees of Freedom Probability Values (P) (N) .95 .90 .80 .70 .50 .30 .20 .10 .05 .01 1 .004 .016 .064 .148 .455 1.07 1.64 2.71 3.84 6.64 2 .103 .211 .446 .713 1.39 2.41 3.22 4.61 5.99 9.21 3 .352 .584 1.00 1.42 2.37 3.66 4.64 6.25 7.82 11.34 X2 values are in the shaded region N value look here P = about 17% If the probability (P) given in the table is high, it is very likely that this would occur by chance, and we have a good “fit”. If P is low, we conclude that it is not likely that the deviation observed would occur by chance alone. Activity: Probability and Chi-Squares With a partner, get 2 pennies ( or any coin). Toss your coins 100 times. Make sure you record on the chart how many HH, HT and TT you got. Using Chi-Square analysis, how “fit was your data? What is the probability of each landing on heads or tails? Heads Tails 1/2 1/2 Heads HH HT 1/2 1/4 1/4 Tails HT TT 1/2 1/4 1/4 Phenotype: 1/4 Heads/Heads: 1/2 Heads/Tails: 1/4 Tails/Tails Punnett Squares work in the same manner Alleles: T = tall What are the possible gametes produced by these parents? Tt T Tt T t Tall Tall TT Tt t = short Phenotype: ¾ or 75% Tall ½ or 25% short t Tall Tt Short tt Genotype: ¼ or 25% TT 2/4 or 50% Tt ¼ or 25% tt Practicing Monohybrid Punnett Squares Exercise: Punnett Squares •Do numbers 1, 2 and 3 – make sure to include phenotype and genotype ratios •Get each problem checked by me before you move to the next With a partner, go to the back of the room and complete the lab. You will need your chi-square chart. Lab – Corn Genetics