Oogenesis Behind every successful embryo stands a hard-working mother. Eggs are enormous cells carrying nutrients for the developing embryo Why? Mass of egg must equal the mass of the embryo that emerges! Even human eggs are huge cells Eggs are enormous cells carrying nutrients for the developing embryo • yolk=large stores of nutrients • large stores of macromolecules • most eggs surrounded by a coat or shell The germ line is set aside early in development as a separate cell lineage "P granule" This can sometimes be visualized by the segregation of putative "determinants” as in C. elegans HOW are P-granules segregated? PAR proteins & microfilaments see p. 246 and Fig. 8.43 Fertilized egg P1 mother cell of germline = P4 C. elegans: P granules are segregated at each division and make the cell that inherits them the germline Figure 8.44 cell nuclei P-granules Similar mechanisms lead to very early segregation of the germline in the fruit fly Drosophila A P Germ cell progenitors The Germline is also set aside in Humans Oocytes arrest in prophase of meiosis I for up to 50 years! Figure 19.23 How does a single egg cell make all the stuff needed to start development? Fly Oogenesis Nurse cells are mitotic sisters of the oocyte Figure 19.4 Nurse cells synthesize macromolecules and pump them into the oocyte through the cytoplasmic bridges follicle cells oocyte nurse cells The Drosophila ovary is composed of somatic follicle cells, germ line nurse cells, and the oocyte Most of the instructions for forming organisms are already in the egg! Asymmetric distribution of egg contents influences embryonic development Molecules synthesized by the nurse cells and specifically localized in the oocyte determine the anterior/posterior axis of the embryo Oocyte Anterior Determinant (bicoid) Specific mRNAs are bound by proteins and transported along microtubules by motor proteins to their destinations Posterior Determinant (nanos) Ventral follicle cells build a signal into the egg shell that determines the dorsal/ventral axis of the embryo Dorsal See Fig. 9.11 for more details Torpedo receptor gurken mRNA Anterior nucleus binds Gurken; signal inhibits Pipe in dorsal follicle cells Posterior Pipe protein Oocyte signals follicle cells, follicle cells signal back to oocyte cytoplasm Ventral Ventral follicle cells Pipe intiates a signaling pathway for activation of Dorsal transcription factor in ventral cells only nucleus Ventral signal initiated by Pipe Transmembrane receptor (Toll) Signaling pathway Transcription factor (Dorsal) relocalized to ventral nuclei of embryo activating new genes Localized mRNAs also influence vertebrate development Vg1 mRNA See also Figure 5.38 Human oogenesis: An example of hormonal control of reproduction Meiosis I 2 million present at birth ovulation Cyclic changes in hormonal signals regulate the timing of female reproduction Release into the Fallopian tube Most mammalsonce/year Humans- monthly (Turns off FSH and LH!) Cyclic changes in hormonal signals regulate the timing of female reproduction RU486- blocks progesterone receptors Plan B-progestin (interferes with ovulation, fertilization, implantation) Progesterone and estrogen birth control pillsprevent maturation of new ova Release into the Fallopian tube (Turns off FSH and LH!) Anton van Leeuwenhoek WNYC’s Radiolab clip from “Sperm” (12/2/08) By Jad Abumrad & Robert Krulwich Figure 7.1 The Human Infant Preformed in the Sperm, as Depicted by Nicolas Hartsoeker (1694) The sperm: a stripped down machine for delivering DNA Figure 7.2 Chemical cues from the egg attract sperm In sea urchins, the cue is resact 0 sec 20 sec 40 sec Figure 7.9 Sperm chemotaxis Inject resact 90 sec Step one: Contact between the sperm and the eggshell Step two: Contact between the sperm and the egg’s plasma membrane (zona pellucida=egg shell) Fertilization: a multistep process Sperm-eggshell contact triggers the acrosome reaction Example 1-- the sea urchin Species specificity (equivalent to zona pellucida) enzymes attracts/activates sperm Figure 7.8 Sperm-eggshell contact triggers the acrosome reaction Example 2: Mammals Eggshell includes ZP3/Sperm have ZP3 receptor Figure 7.8 BLOCKING POLYSPERMY 1. Fast/transient: prevent sperm fusion 2. Slow/permanent: removal of other bound sperm Slow block 20-60 seconds permanent Fast block 1-3 seconds lasts 1 min How do we know this? We can measure these events directly! (sperm can’t fuse with egg) +20 mV resting -70 (sperm can fuse with egg) Figure 7.17 Membrane potential of sea urchin eggs before and After fertilization. Blocking polyspermy: SLOW BLOCK Figure 7.6 Cortical granules: poised for release 15,000 cortical granules in a sea urchin egg • contain enzymes that clip the egg’s bindin receptor and any attached sperm • alter the vitelline envelope (zona pellucida in mammals so that ZP3 receptor on sperm cannot bind) Sperm-eggshell contact triggers the acrosome reaction Example 2: Mammals Sperm have ZP3 receptor A wave of increased calcium can be visualized moving across the egg from the point of sperm entry Figure 7.20 Slow block 20-60 seconds permanent Fast block 1-3 seconds lasts 1 min pH 1 0-5 7.4 Ca 2+ 1 0-6 7.2 intracellular calcium intracellular pH 1 0-7 7.0 1 0-8 6.8 200 400 600 time (seconds)