Agenda 11/17/14- Photosynthesis • Cellular Respiration Quiz • Intro to Photosynthesis worksheet (work on when you finish quiz) • New Information: Photosynthesis and Plant Cell Specialization • Photosynthesis Diagram worksheet • HW- finish packet Photosynthesis Video • As you watch, consider the following: – Where does the energy that drives photosynthesis come from? – What molecules are formed during photosynthesis? – What 2 molecules carry energy that is eventually stored in glucose? – What kinds of organisms contain chloroplasts? THE BASICS OF PHOTOSYNTHESIS • Most plants are photosynthetic autotrophs, as are some bacteria and protists – Autotrophs: generate their own organic matter through photosynthesis – Sunlight energy is transformed into chemical energy stored in chemical bonds (a) Mosses, ferns, and flowering plants (b) Kelp (c) Euglena (d) Cyanobacteria Light Energy Harvested by Photosynthetic Autotrophs 6 CO2 + 6 H2O + light energy → C6H12O6 + 6 O2 Food Chain Waxy coating Blade Vein Stem • The location and structure of chloroplasts Chloroplast LEAF CROSS SECTION MESOPHYLL CELL LEAF Mesophyll CHLOROPLAST Intermembrane space Outer membrane Granum Grana Stroma Inner membrane Stroma Thylakoid Thylakoid compartment Chloroplasts Intermembrane space Thylakoid Outer membrane Granum Inner membrane Stroma A Chloroplast Light Energy H2O CO2 Chloroplast NADP+ ADP Grana (stacks of thylakoids) LightDependent Reactions Calvin Cycle ATP NADPH O2 Sugars Chemical Energy 2 Pathways of Photosynthesis • Light-dependent reactions – Light energy absorbed – Occurs across thylakoid membranes – Produces ATP and NADPH (and O2) • Light-independent reactions or Calvin Cycle – Does not require light energy – Occurs in the stroma – Produces glucose using CO2 ATP ATP - Adenosine Triphosphate Adenine Ribose 3 Phosphate groups Formation of ATP ADP ATP Energy Adenosine diphosphate (ADP) + Phosphate Partially charged battery Adenosine triphosphate (ATP) Fully charged battery ATP stores enough energy for many cellular functions. A Chloroplast Light Energy H2O CO2 Chloroplast NADP+ ADP Grana LightDependent Reactions Calvin Cycle ATP NADPH O2 Sugars Chemical Energy H2O CO2 Sunlight Energy NADP+ ADP Light Independent Reactions in the Stroma Light Dependent Reactions in the Thylakoids ATP NADPH O2 C6H12O6 Light Dependent Reactions • Pigments arranged into photosystems – Photosystem I (happens 2nd) – Photosystem II (happens 1st) • Inputs: – H2O – Light Energy – ADP, NADP+ • Products: – ATP, NADPH (go on to fuel the Calvin Cycle) – O2 Light Dependent Reactions • Electron transport chain – Pumps H+ ions across the thylakoid membrane- results in energy for ATP production • ATP Synthase (enzyme) – H+ ions pass through the thylakoid membrane via ATP synthase, making it spin (energy) – ATP synthase spins, binding ADP and a phosphate group into ATP The Two Photosystems of the Light Reactions: P-680 and P-700 Potential Energy Off to the Calvin Cycle H2O CO2 Sunlight Energy NADP+ ADP Stroma Thylakoids ATP NADPH O2 C6H12O6 Light-Independent Reactions, aka Calvin Cycle • occurs in stroma • uses ATP and NADPH from the light-dependent reactions and CO2 to make the organic molecule glucose (C6H12O6) The Calvin Cycle CO2 Enters the Cycle Energy Input •Inputs: • ATP • NADPH • CO2 • Products: • C6H12O6 • ADP • NADP+ 5-Carbon Molecules Regenerated 6-Carbon Sugar Produced Sugars and other compounds H2O CO2 Sunlight Energy NADP+ ADP Stroma Thylakoids ATP NADPH O2 C6H12O6 Agenda 11/19/14- Photosynthesis and Plant Pigments • Bellwork • New Information- Photosynthesis and Properties of Light, Endosymbiotic Theory • Lab- Plant Pigments and Chromatography • HW- finish lab (if possible), formal lab report due Monday. • What is the primary role of photosynthesis in the carbon cycle? A. releasing carbon to the oceans B. removing carbon from the atmosphere C. transferring carbon from the soil to organisms D. transferring carbon from producers to decomposers Some types of bacteria are able to perform photosynthesis. These bacteria must therefore contain which of the following in their membranes? • A. chlorophyll • B. glucose • C. mitochondria • D. ribosomes Properties of Light • when a photon (unit of light) strikes a substance, it may – be reflected – be absorbed – be transmitted • Plant pigments absorb light energy. – This fuels the production of ATP, NADPH Why Are Plants Green? Electromagnetic Spectrum and Visible Light Gamma rays X-rays UV Infrared & Microwaves Visible light Wavelength (nm) Radio waves WHY ARE PLANTS GREEN? Different wavelengths of visible light are seen by the human eye as different colors. Gamma rays X-rays UV Infrared Visible light Wavelength (nm) Microwaves Radio waves Why are plants green? Because they reflect green light The color of light seen is the color REFLECTED, NOT ABSORBED • Chloroplasts have pigments that absorb light energy and convert it to chemical energy • What colors of light does chlorophyll absorb? Reflect? Light Reflected light Absorbed light Transmitted light Chloroplast Paper Chromatography Lab • What pigments can be found in leaves? Chloroplast Pigments • Thylakoids in chloroplasts contain several pigments • • • • • Chlorophyll a (bright green) Chlorophyll b (yellow-green) Carotenoids (yellow/orange) Xanthophylls (yellow) Anthocyanins (red) Figure 7.7 Different pigments absorb light differently Green to Greenishyellow Yellow/Orange/R ed Why do plants have pigments other than chlorophyll? Green to Greenishyellow Yellow/Orange/R ed Why do plants have pigments other than chlorophyll? • To absorb more energy – from wavelengths (colors) of light that chlorophyll does not absorb. • This allows them to make more food! Chlorophyll Photosynthesis Activity Site Endosymbiotic Theory (Lynn Margulis, 1981) • Mitochondria and chloroplasts may have originated as free-living prokaryotes that lived symbiotically within cells, leading to eukaryotes. • Evidence: – – – – Circular DNA Reproduction Two or more cell membranes Ribosomes animation Agenda 11/20- Photosynthesis and Cellular Respiration • Bellwork • Finish Lab- calculate Rf values, answer lab questions, discuss lab report • Review for test Spinach Leaf Pigments Distance travelled by solvent: 65 mm Concept Map Section 8-3 Photosynthesis includes Lightdependent reactions Calvin cycle use take place in Energy from sunlight Thylakoid membranes to produce ATP Go to Section: NADPH O2 takes place in Stroma uses ATP NADPH of to produce Chloroplasts High-energy sugars Compare/Contrast Photosynthesis Cellular Respiration Energy Storing or Releasing Energy storing Energy releasing Products Glucose, oxygen gas Carbon dioxide, water, energy (ATP) Reactants Carbon dioxide, water, light energy Glucose, oxygen gas Location Chloroplast Mitochondrion