BIO 215-01: MICROBIOLOGY
FALL 2014 SYLLABUS – 15 WEEKS
M 8:00-10:30am, SCOTT 751
W 8:00-10:30am, SCOTT 721
LECTURE/LAB HOURS/CREDITS: 3/3/4
INSTRUCTIONAL METHOD: Web Enhanced
Vasant Chary, Ph.D, Adjunct Instructor
vchary@rcgc.edu
Office Hours: By Appointment
It is the responsibility of the student to review the RCGC Information and Policies, as well as the
Online Syllabus at www.rcgc.edu/syllabi
TEXTBOOK AND COURSE MATERIALS
• Textbook: Microbiology, A Systems Approach by Cowan, 4th Edition, ISBN #978007302437,
Publisher: McGraw-Hill
• Lab Manual: Microbiology, Custom Edition, ISBN #2811002010793, RCGC Bookstore
• Safety Glasses, RCGC Bookstore
Please see current textbook prices at www.rcgc.bncollege.com
EVALUATION AND ASSESSMENT
Grading Distribution
•
•
•
•
•
Tests (4 @100 pts. each)
Quizzes (5 @ 10 pts. each)
Lab Assignments (12 @ 5 pts. each)
Student Presentation
Attendance & Class participation
TOTAL
* Lowest quiz grade would be dropped
Grading Scale
The following grading scale will be used in this course:
93% - 100%
91% - 92%
89% - 90%
83% - 88%
81% - 82%
79% - 80%
=
=
=
=
=
=
A
AB+
B
BC+
73% 71% 69% 63% Below
400 points
40 points*
60 points
65 points
35 points
600 points
78%
72%
70%
68%
63%
=
=
=
=
=
C
CD+
D
F
2
BIO 215 CORE COMPETENCIES
This course focuses on two of RCGC’s Core Competencies:
• Scientific Knowledge and Reasoning
• Quantitative Knowledge and Skills
STUDENT LEARNING OUTCOMES: BIO 215: MICROBIOLOGY
Successful completion of BIO 215 will
help students:
RCGC Core
Competencies
Evaluation / Assessment
1. Distinguish among the various groups
of microorganisms within the microbial
world.
- Scientific Knowledge
and Reasoning
- Exams
2. Develop the skills of handling,
observing, culturing and staining
microorganisms.
- Scientific Knowledge
and Reasoning
- Laboratory assignments
3. Describe the main characteristics of
bacteria, including their morphology,
growth, reproduction and classification.
- Scientific Knowledge
and Reasoning
- Quantitative
Knowledge and Skills
- Exams
- Quizzes
4. Understand and apply methods of
microbial control that can be used to
prevent both the transmission of
infectious diseases and the spoilage of
foods.
- Scientific Knowledge
and Reasoning
- Quantitative
Knowledge and Skills
- Exams
- Quizzes
- Laboratory assignments
5. Understand and describe the
fundamental principles of the hostparasite relationship both in health and
disease.
- Scientific Knowledge
and Reasoning
- Quantitative
Knowledge and Skills
- Exams
- Quizzes
- Laboratory assignments
6. Describe the components of the human
immune system and understand how
these components interact in
generating an immune response.
- Scientific Knowledge
and Reasoning
- Quantitative
Knowledge and Skills
- Exams
- Quizzes
- Laboratory assignments
COURSE POLICIES
ATTENDANCE – Students attain maximum academic benefit through regular class attendance.
Therefore, students are expected to attend all class sessions for which they are scheduled.
Attendance will be recorded. If you miss a class, it is your responsibility to make up all material
missed. You should make arrangements with someone who attended the class to get copies of
handouts and notes.
EXAMS/QUIZZES – Exams and quizzes will be based on lecture notes, laboratory exercises,
assigned textbook reading and handout sheets and could include multiple choice and essay
3
questions. The student is responsible for assigned textbook readings to prepare you for tests
and quizzes.
MAKE UP POLICY – There are no make-ups for exams or quizzes.
PARTICIPATION AND ARTICLES – Students may elect to find articles regarding current topics
in the news pertaining to any microbiology issue. Students will bring in the article(s) and be able
to discuss the article with the class. Students can do this two times during the semester in order
to receive +5 points each extra credit. These articles can be found in local papers, on the web,
etc.
STUDENT PRESENTATIONS – All students are required to present a formal PowerPoint
presentation on a disease found in chapters 18-23 of the Microbiology textbook. Presentations
will occur over several class periods at the end of the semester. Students may work individually
or in groups of 2 but each student must present a portion of the lecture. A schedule with topics
to choose from and a list of the required elements are posted on eLearning listed under “student
presentations.”
ACADEMIC INTEGRITY – In cases of cheating, plagiarism and/or fabrication, faculty have the
authority to impose an academic sanction that is reasonable and commensurate with the
violation (Rules and Regulations #R-8019). Students will receive grade of “0” for cheating
violation. Make sure you reference your sources of information, including internet sources.
ELECTRONICS – Use of cell phones, MP3 players, pagers, and similar electronic devices is not
permitted during class time. Approval must be gained from the instructor prior to student use of
audio or video recording devices in class.
BIO 215 MICROBIOLOGY SYLLABUS
1. Introduction to Microbiology – Science of Microbiology, Microbial Cells. Evolution and
Extent of Microbial cells. Pathways of Discovery in Microbiology (Contributions of
Leeuwenhoek, Pasteur, Koch etc.,). Overview of microbial Diversity. Modern era of
Microbiology.
2. Cell Structure and Function (Cell Shape and Size, Cytoplasmic membrane and
transport, Cell Walls of Prokaryotes, Cell surface structures, Microbial locomotion.
3. Microbial Metabolism. Nutrition and culture of microorganisms. Essentials of
Catabolism (Aerobic vs Anaerobic respiration vs Fermentation)
4. Microbial growth. Dynamics of bacterial growth and measurement.
5. Control of Microbial growth (Selection of Antimicrobial procedures. Heat, Chemical
and Physical methods of growth control).
6. Antimicrobial agents. Antibiotics. Mode of action of antibiotics and mechanisms of
antibiotic resistance. Determinations of MIC & Susceptibility testing)
7. Viruses and Virology. Virus Structure and Growth. Viral replication. Overview of
bacterial viruses. T2 and Temperate bacteriophages, Lambda.
8. Microbial interactions with humans – An overview. Normal micro flora of Skin, Oral
cavity, GI and other body regions.
9. Microbial virulence and Pathogenesis. Host microbe interactions. Mechanism of
Pathogenesis Invasion, colonization and infection. Exotoxins and Endotoxins.
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10. Immunology. Immunity and Host Defense. Innate and Acquired immunity. Antigen and
Antibody – Structure and function.
11. Applications of Immune Response (Vaccines and Immunization Procedures).
12. Microbial diseases. Important human diseases caused by microorganisms. For eg:
Skin & Wound infections caused by Bacteria and Viruses. Respiratory infections by
bacteria and Viruses. Diseases of the digestive system. Sexually transmitted diseases.
Vector-borne microbial pathogens. Human diseases caused by Protozoa and Fungi.
Emphasis would be on microorganism involved, mode of transmission, prevention and
control.
LECTURE NOTES
Lecture outlines and other class materials are available on my webpage:
https://drvasantchary.wikispaces.com.
Instructions to open and download the lecture file from the Weblink
1.
2.
3.
4.
Web link: https://drvasantchary.wikispaces.com/
Click ‘Pages and Files’ menu present on the top right hand side.
Single click on any of the Files you need. - A new Window opens
Click ‘download’ button present on the top right hand side.
DOWNLOAD NOTES A DAY BEFORE LECTURE CLASS. The lectures are PowerPoint
presentations, so you will need to select from the Format option when printing them out. Most
students prefer either the Outline Format or the Handouts Format. With the Handouts format,
specify 3 slides per page and you will get lines to write notes on. If you do not select an option,
the notes will print out as 1 slide per page and this is not a very useful format. Lecture and lab
notes will also be posted on the Wikispaces web site for your reference.
HOW TO SUCCEED IN BIO 215- MICROBIOLOGY:
1. Plan study time into your schedule! You will need 6-8 hours of study time per week. I
think it is more effective to do several shorter study sessions per week rather than one
very long weekly study session. The main idea to keep in mind is that science is not a
subject that can be learned by trying to read five chapters the day before the exam.
2. What to study: You will need to coordinate lecture and text materials as you study and
do the review questions at the end of each chapter. Most students need to read each
chapter twice to really understand and remember the material. Don’t forget to study the
Figures in the text. Use “chapter key word” and “outline” files posted on the Canvas
website to take notes while you read.
3. When to study: I’d strongly recommend that you come prepared for the class by
reading the material a day before the lecture class. You may not understand everything,
but that is fine. By reading beforehand you become familiar with certain terms and
concepts that would greatly help you in understanding them in depth during the class.
Write down some questions that you would like to discuss during the class as it would
help you actively participate during classroom discussions. This is called active learning
process that would be included for a portion of grading.
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4. Find your own best study technique: use flashcards, draw diagrams if you are a
visual learner, record and listen to your notes if you remember what you hear. Make
studying more social by forming study groups.
LECTURE SCHEDULE*
Date
Topic
Reading
Chapters
Section 1: Cell Structure and Function
Sept.03 (W)
Introduction into the Course
Chapter 1
(W)
Sept.08
(M)
The Themes of Microbiology
Chapter 1
Sept.10 (W)
Biomolecules - The Chemistry of Biology
Chapter 2
Sept.15 (M)
Prokaryotic Cell – Part 1
Chapter 3
Sept.17 (W)
Prokaryotic Cell – Part 2
Chapter 3
Sept.22 (M)
Eukaryotic Cell
Chapter 4
Sept.24 (W) Buffer day** & Review day for the Unit 1 Exam
---Sept.29 (M)
No Class on this day
---Section II: Microbial Growth, Metabolism & Viruses
Oct.01 (W)
Section II EXAM
---Oct.06 (M)
Methods of Culturing Microorganisms Chapter 5
Oct.08 (W)
Microbial Nutrition
Chapter 7
Oct.13 (M)
Microbial Growth
Chapter 7
Oct.15 (W)
Metabolism
Chapter 8
Oct.20 (M)
Viruses – Part 1
Chapter 6
Oct.22 (W)
Viruses – Part 2
Chapter 6
Oct.27 (M)
Buffer day** & Review day for the Unit 2 Exam
---Oct.29 (W)
College Day classes - Closed
---Section III: Microbial Control, Antibiotics & Host defense mechanisms
Nov.03 (M)
Section III EXAM
---Nov.05 (W)
Microbial Control - Physical & Chemical
Chapter 11
Nov.10 (M)
Principles of Chemotherapy Chapter 12
Nov.12 (W)
Microbe-Human Interactions
Chapter 13
(Antibiotics
and Drug -resistance)
Nov.17 (M)
Host defense
mechanisms
Specific & NonChapter 14
Nov.19 (W)
Host defense mechanisms
Chapter 14
specific - Specific & NonNov.24 (M)
Buffer day** & Review
specific
day for the Unit 3 Exam
---Nov.26 (W)
Section III Exam
---Section IV: Immunology and Infectious Diseases
Dec.01 (M)
Immunity: Steps 1 and 2
Chapter 15
Dec.03 (W)
Immunity: Steps 3 and 4 (Immunization)
Chapter 15
Dec.08 (M)
Human Infectious diseases – Part 1
Chapters 16-23
Dec.10 (W)
Human Infectious diseases – Part 2
Chapters 16-23
Dec.15 (M)
Buffer day** & Review day for the Final Exam
---Dec.17 (W)
Section IV - FINAL EXAM
---* This schedule is tentative. Some lecture classes may not be covered on the days specified.
** Buffer day is used for completion of any incomplete lecture class. It would also be used for
review of the completed chapters for the unit test that follows
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LABORATORY OUTLINE
I.
II.
III.
IV.
All lab experiments are held on Wednesdays of the week except for Oct 27th
which would be held on Monday of that week
Laboratories will be completed and handed in the day they are completed in their
entirety.
Students may work in groups of 3-4 and should remain lab partners for the
semester, as you will be sharing lab equipment
The following labs will be covered during this semester (see calendar)
DATE
LAB/EXP
NUMBER
10th Sept.
1&2
17th Sept.
3&4
th
LAB EXPERIMENT
Introduction to Laboratory Safety & Microscopy
Examining Live and Fixed Bacterial Preparations
Simple Staining, Negative Staining
24 Sept.
5&6
1st Oct
7&8
8th Oct.
9
Gram Staining and Acid-Fast Staining
Streak Plate & Spread Plate Technique/ Culturing
Microorganisms
Chemical Agents of Microbial Control
27th Oct**
5th Nov.
12th Nov.
10
11
12
Protista, Fungus and Worms
Epidemiology Simulation and Lab 12 swab distribution
Contaminants at home
19th Nov.
13
Identify Disease - Bacterial DNA
** Lab on Monday – FOR THIS WEEK ONLY
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STUDENT LABORATORY RULES
1.
Equipment location will be identified for students by the instructor.
2.
Students will be assigned microscopes and stain kits to use for the duration of the
semester. Students are responsible for taking care of equipment and are to report any
damage to the instructor immediately.
3.
Food and beverages are not permitted on the lab benches.
4.
All tables to be cleaned up at the end of each laboratory session. All wastes must be
properly disposed of; i.e., paper towels in wastebasket.
5.
All chairs to be put in place under table when leaving.
6.
Each student shall have the responsibility for the cleanliness and maintenance of the
laboratories within the framework of mutual respect and professional ethics.
7.
Absences from a lab counts as an overcut in the course. No make-up labs are available
by which the absence can be erased. The student is still responsible for course content
covered by the lab that was missed.
8.
BEFORE YOU LEAVE THE LAB:
a. Be sure the class is officially excused.
b. Store your microscope properly.
c. Return other materials, if any, to proper storage, as directed. (If you are not sure-ASK!)
d. Clean tabletop completely (E. alcohol). Wipe down the counter with a damp paper
towel.
e. Push lab chair under the table at your place.
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RUBRIC FOR ASSESSING PRESENTATIONS
Introduction to
Topic
Explanation of
Scientific
Studies
D
Beginning or
Incomplete
Very little
background
information
provided or
information is
incorrect
No primary
research
explained or noncredible
unscientific
research
sources.
C
Developing
B
Accomplished
A
Exemplary
Some introductory Introduction is nearly
information, but
complete, missing
still missing some some minor points
major points
Introduction
complete and wellwritten; provides all
necessary
background
principles for the
talk.
Primary research
studies are
presented but
many are
misstated,
indicating a lack of
understanding or
studies are not
explained in an
interpretable
manner to the rest
of the class.
Study results have
been interpreted
correctly and
discussed, good
understanding of
results is conveyed
All important
conclusions have
been drawn from
studies, could be
better stated
Score
All important
conclusions have
been clearly made,
individual shows
good understanding.
Some studies
mentioned but
briefly and not
explained.
Oral
Communication
Presentation is
rough and
incomplete.
Presentation is
confusing and
difficult to follow.
Presentation follows
a logical progression
but could be
improved. Audience
had difficulty
understanding.
All information
communicated well
and understood by
audience.
PowerPoint
Sections difficult
Appearance and to follow,
formatting
Presentation
does not make
sense.
Slides can be
read but with
difficulty. Slides
do not help
audience follow
along.
Slides are readable
but could still be
improved as a more
effective visual aid.
All slides are wellformatted and very
readable. Easy to
follow
References
Sited
Sources not
properly sited and
less than 3
scientific
resources
Three or more
scientific sources but
not properly sited
Three or more
scientific sources
properly sited
No references
sited
Total Score: _______