COURSE
TITLE
BST
1
0
1
Microbiology
L
T
P
CREDITS
(or Max.
Marks)
4
0
1
5
COURSE OBJECTIVES: To acquaint students with knowledge of microbiology. It would
enable them to understand the microbiology & its application, which will further help them
to pursue a career in the relevant field.
PRE-REQUISITES:
COURSE CONTENTS:
Unit
1
Description
History, Scope & Introduction of microbiology: A.Leewenhook,, L.Pasteur,
R.Koch, J.Lister, J.Tyndall,Biogenesis Vs Abiogenesis, Koch Postulates,
Discovery of antibiotics.
Principle of Microscopy & applications:
Bright fields, Dark field, Phase contrast, Fluorescent & Electron microscopy.
Morphological Classification based Staining Techniques:
Positive, Negative, Capsule, Acid fast, Gram staining.
Prokaryotes
A general account on characteristics, structure, Reproduction & functions of Bacteria
(Microbial spores: sporulation/germination process) Archaebacteria
Cyanobacteria, mycoplasma.
Eukaryotes:
A general account on characteristics, Structure, Reproduction, Functions of yeast,
Molds, Slime molds, Algae.
2
Microbial Growth & nutrition:
Requirement of nutrients for microbes, Nutritional categories among, microorganisms
Choice of media & incubation
Pure Culture Techniques:
Methods of culturing aerobic & anaerobic bacteria, Sterilization Techniques.
Microbial Growth:
Growth curve (phases of growth), Generation time, Growth rate, mono-auxic, diauxic & synchronous growth, Chemostat & turbidostat.
Normal Micro-flora in humans/animals:
Types of microbial pathogens & diseases caused by them, Microbial Interactions like
symbiosis & antibiosis etc.
3
Microbial Genetics:
Introduction to microbial genetics, Modes of bacterial conjugation, transduction
transformation.
Economic Importance:
Importance of Prokaryotes & eukaryotes, animal (1H1N1, Hepatitis, HIV, HSV1 and HSV-2, Dengue) & Plant viruses (Wheat Yellow Mosaic, Barley Yellow Dwarf
of Wheat, Oats, and Barley, Tobacco Mosaic Virus, Cucumber Mosaic Virus)
Structure & reproduction of above mentioned viruses, causative agent, disease caused
by these viruses
Suggested Readings:
1. Tortora, G.J., Funke, B.R. and Case, C.L. (2009) Microbiology: An introduction (Benjamin/
Cummings publishing company, Inc).
2. R. Y. Stanier, M. Doudoroff, E. A. Adelberg (1999). General microbiology (MacMillian Press
London).
3. M.J. Pelczar, E.C. Sun Chan, N.R. Krieg (1986). Microbiology (Tata McGraw Hill
Publication, New Delhi).
4. H.G. Schlegel, C.Zaborosch, M. Kogut (1993). General microbiology (Cambridge University
Press).
5. S.C. Prescott, C.G. Dunn (1959). Industrial microbiology (McGraw- Hill).
6. Purohit, S.S. (2003). Microbiology: Fundamentals and applications(Agrobios, India)
7. Postgate, J.R. (2000). Microbes and man (Cambridge Uni)
Instructions for paper setter.
The syllabus has been divided into three equal units. The paper setter is required to set Ten
questions in all, three questions from each unit and a compulsory question consisting of five sub
parts and based on the whole syllabus. The candidate will be required to attempt six questions
including the compulsory question number no 1 and not more than two questions from each unit.