Unit 3: Mitosis and Meiosis Internet Activity ONION ROOT TIPS AND THE CELL CYCLE Actively dividing cells pass through a predictable series of stages called the cell cycle. A cell cycle starts with interphase, just after a new cell is formed by mitosis. During interphase, the cell duplicates its DNA and increases its mass and number of cytoplasmic components. This prepares the cell for the next round of mitosis. The cell cycle ends when the cell divides. If you look under a microscope at cells from actively dividing tissue, you will find that their cell cycles are not synchronized. Some cells are in interphase, while others are in the various stages of mitosis. By noting the number of cells in each stage, it is possible to calculate the relative lengths of each phase of the cell cycle. Activity In this activity, you will calculate the lengths of the various phases of the cell cycle in an onion root tip. First you will view some slides and graphics of onion root tips provided by the Molecular Expressions Photo Gallery. Then you will complete the online activity provided by the Biology Project at the University of Arizona. Once you have completed this exercise, you will play the Cell Cycle game. PART 1 Use your browser to go to Molecular Expressions Photo Gallery: Mitosis http://www.microscopy.fsu.edu/micro/gallery/mitosis/mitosis.html. Notice in the micrograph at the top of the page that there are cells in a variety of stages. View the onion slides in this section. Once you have read this entire page, click on "mitosis" in the sentence "Learn the steps in mitosis ...” This will take you to a Java tutorial. Keep in mind what you have learned here as you proceed to the second part of the activity. PART 2 Use your browser to go to Online Onion Root Tips at http://www.biology.arizona.edu/cell_bio/activities/cell_cycle/cell_cycle.html. Begin by reading the description of the five major cell phases. You will need to keep this information in mind during the activity. Keep your own copy of your data using the data table provided. You will need it to answer the questions. Proceed through the activity, identifying the phase for each cell you are shown. Pay attention to the hints if you misidentify a cell at first. PART 3: When you have completed the worksheet, including the drawings and the questions, go to the following website and work through the Cell Cycle game. http://nobelprize.org/educational_games/medicine/2001/cellcycle.html You are on your honor to try your best to complete the game. Unit 3: Mitosis and Meiosis Name: _________________ Internet Activity ONION ROOT TIPS AND THE CELL CYCLE After you have correctly identified the phases, sketch a picture of the cells going through each phase. You must include at least 4 different chromosomes in the nucleus in your sketches: Interphase: Prophase: Metaphase: Anaphase: Telophase: Data Table: Cell Cycle Interphase Prophase Metaphase Anaphase Telophase Total number of cells 36 percent of cells 100% When you have completed the activity, answer the following questions: 1. What percent of cells were in interphase? 2. What percent were in mitosis? 3. Which phase of mitosis takes the longest? 4. During which stage is the nucleolus visible as a dark spot? 5. How can you recognize a cell in metaphase?