Heartland Community College
Fall 2011 - Biology 191 – Introductory Microbiology Lab
Lab Sections
L2 – TR 9:00 – 10:50 AM
L5 – TR 1:00 – 2:50 PM
Instructor Information:
Jon Hoekstra Office: 2414 ICB
HCC Phone – 309-268-8648 E-mail – jon.hoekstra@heartland.edu
Office hours – MTWR 11:00 AM – 12:00 PM; MW 4:00 – 4:50 PM
Laboratory Manual Information
Students may obtain lab exercise instructions, pre-lab assignments, and final lab reports by printing
instructions from the course website at this address:
http://employee.heartland.edu/SROSE01/BIOL191/Lab/lab.index.htm
Notice of Cancelled Class Sessions
Cancelled class sessions for all HCC classes will be listed under Cancelled Class Meetings in the A-Z
Index and under Academic Information in the Current Students page on the HCC Web site. Go to
http://www.heartland.edu/classCancellations/ to learn what classes have been cancelled for that day and the
upcoming week. Be sure to check the last column, which might contain a message from the instructor.
Pre-lab Reports, Lab Reports, and Exams
Excluding the Microscopy, Epidemiology, CD-ROM labs, exercises contain two assignments, the PREREPORT and the LAB REPORT. The Pre-Report should be completed before the day the exercise BEGINS.
It will be collected AT THE BEGINNING of the lab period before the pre-lab instruction. The purpose of the
pre-report is to encourage students to read the lab before coming to class. It will not be accepted late. The
Final Lab Report should be completed and turned in immediately after the lab is completed. Because lab work
is “hands-on”, your presence in class is necessary. Therefore, if you are absent, the pre-report and/or lab report
due that day will not be accepted. There are no make-up labs.
The laboratory portion of the course comprises 25% of the total BIOL 191 grade. However, a grade of 70% or
higher in the lab portion is required in order to pass the course. Failure to achieve 70% or more in the lab
portion will result in a grade of “F” for the entire course regardless of the grade in the lecture portion.
Points for the laboratory portion of the course:
Assessment
Microscopy Lab Report
Pre-reports (10 @ 5pt. Each)
Lab reports (11 @ 5pt. Each)
CD-ROM labs (2 @ 5pt. Each)
Video Worksheet
Quizzes (5 @ 25pt. Each)
Unknown Characteristic Chart
Epidemiology Journal
Epidemiology Oral Presentation
TOTAL AVAILABLE:
BASIS FOR GRADE:
Points
10
50
55
10
10
125
30
20
20
330 pts.
320 pts.
Microbiology Lab Schedule Fall 2011
Date
Week 1
8/23
8/25
Exercises
PL = prelab due; LR = report due
Introduction to the lab
Video: Bacteria
Microscopy LR
Day 1 - Widespread Distribution PL
Objectives
Lab schedule, safety regulations, laboratory
equipment and student evaluation are
discussed.
Principles of microscopy are introduced.
Students learn to utilize the compound
microscope and immersion oil techniques.
Video: The Tree of Life
Students examine the diversity and ubiquitous
nature of bacteria. Aseptic technique is
introduced.
9/1
Day 2 - Widespread Distribution of
Microorganisms LR
Students learn simple staining techniques and
observe bacterial cells using the microscope.
Week 3
9/6
LABOR DAY – NO LAB (Tuesday LABS are “off” to maintain weekly schedule)
9/8
Day 1 - Pure Cultures & Aseptic Tech. PL
Week 2
8/30
Culturing procedures are performed utilizing
a mixed culture.
QUIZ 1
Week 4
9/13
Day 2 - Pure Cultures & Aseptic Technique
CD-ROM: Biochemistry: The Chemistry of
Living Things WS
Day 3 - Pure Cultures & Aseptic LR
9/15
Week 5
9/20
Video: E. coli: Confessions of a Germ
Day 1 - Sampling Food, Water, Milk PL
Day 1 - Intestinal Pathogens PL
Day 2 - Sampling Food, Water and Milk LR
Students observe colonies from mixed
samples and attempt to isolate pure cultures.
Students perform interactive tutorials.
Students observe pure cultures and view them
under the microscope.
Sampling procedures are performed.
Students attempt to isolate intestinal
pathogens on selective media.
Colony counts and presumptive determination
of contaminating microorganisms are done.
9/22
Day 2 - Intestinal Pathogens continued
Fermentation tests are performed on selected
intestinal pathogens
Date
Exercises
PL = prelab due; LR = report due
Objectives
QUIZ 2
Week 6
9/27
9/29
Week 7
10/4
Day 3 - Intestinal Pathogens continued
Final inoculations will be performed on
differential media
Day 1 - Control of Microbial Growth:
Alcohol & Hand Scrubbing PL
Students begin testing the effectiveness of
alcohol swabs and hand scrubbing
Day 4 - Intestinal Pathogens LR
Final evaluation of isolated intestinal
pathogens
Day 2 - Control of Microbial Growth:
Alcohol & Hand Scrubbing LR
Day 1 - Control of Microbial Growth:
Evaluation of Antimicrobics PL
Video: Super Staph: Invincible Bacteria?
Day 2 - Evaluation of Antimicrobics LR
10/6
Week 8
10/11
Day 1 - Isolation of an Antibiotic Producer
from Soil PL
Day 2 Isolation of an Antibiotic Producer
from Soil continued
Students evaluate the effects of alcohol and
hand scrubbing
Students begin tests to determine the
effectiveness of specific disinfectants,
antiseptics, and antibiotics
Students evaluate the effectiveness of the
antimicrobics.
Students begin primary isolation of antibiotic
producing bacteria from soil
Students will select antibiotic producing
colonies from soil cultures
Video: Creators of the Future
QUIZ 3
10/13
Week 9
10/18
Day 3 - Isolation of an Antibiotic Producer
from Soil LR
Day 1 – Transformation PL
CD-ROM: Microbes in Motion WS
Students will observe soil cultures for
evidence of antibiosis
Students will begin experiments which will
lead to the genetic transformation of E. coli
Students will perform interactive tutorials
Day 2 – Transformation LR
Students will observe transformed cultures
DNA Extraction LR
Students will isolate bacterial DNA
10/20
Date
Exercises
PL = prelab due; LR = report due
Objectives
QUIZ 4
Week 10
10/25
Day 1 - Preparing Unknown Cultures:
Morphological Characteristics PL
Morphological characteristics of an unknown
bacterial culture are examined.
10/27
Day 2 - Preparing Unknown Cultures:
Morphological Characteristics LR
Morphological characteristics of the unknown are
examined and described.
Week 11
11/1
Day 1 - Unknown Cultures:
Physiological Characteristics PL
Students test biochemical characteristics of their
unknown. Students become familiar with
problems involved in determining the identity of
an unknown and learn about resource manuals.
11/3
Day 2 - Physiological Characteristics of
the Unknown LR
Students complete biochemical testing of their
unknown. References needed to determine the
unknown are utilized.
Week 12
11/8
QUIZ 5
Video: And the Band Played On, Part 1
11/10
And the Band Played On, Part 2 LR
Unknown Characteristics Chart due
Week 13
11/15
Epidemiology Part 1
Students begin recreating an investigation
involving an outbreak of a mysterious illness
Epidemiology Part 2
Students continue their investigation.
Week 14
11/22
Epidemiology Part 3
Students complete investigation and begin
preparations for the oral presentation.
11/24
THANKSGIVING HOLIDAY – NO LAB
Week 15
11/29
Epidemiology Part 4
11/17
Teams continue preparing for the oral
presentation.
Epidemiology Part 5
Student teams perform their oral presentations.
12/1
Video: Dangerous Friends, Friendly
Enemies
Epidemiology Journals due
HANDY TABLE FOR TRACKING YOUR GRADE
Points
Earned
Lab Exercise
Points
Possible
Microscopy
lab report
10
Widespread Distribution of Microorganisms
pre-lab
5
Widespread Distribution of Microorganisms
lab report
5
Biochemistry CD-ROM
5
Quiz 1
25
Pure Culture & Aseptic Technique
pre-lab
5
Pure Culture & Aseptic Technique
lab report
5
Sampling Food, Water, & Milk
pre-lab
5
Sampling Food, Water, & Milk
lab report
5
Quiz 2
25
Intestinal Pathogens
pre-lab
5
Intestinal Pathogens
lab report
5
Alcohol Evaluation & Hand Scrubbing
pre-lab
5
Alcohol Evaluation & Hand Scrubbing
lab report
5
Evaluation of Antimicrobics
pre-lab
5
Evaluation of Antimicrobics
lab report
5
Quiz 3
25
Antibiotic Producer from Soil
pre-lab
5
Antibiotic Producer from Soil
lab report
5
Transformation
pre-lab
5
Transformation
lab report
5
DNA Extraction
lab report
5
Microbes in Motion CD-ROM
5
Quiz 4
25
Video: And the Band Played On
lab report
10
Unknown Cultures-Morphological Characteristics
pre-lab
5
Unknown Cultures-Morphological Characteristics
lab report
5
Physiological Characteristics of the Unknown
pre-lab
5
Physiological Characteristics of the Unknown
lab report
5
Unknown Characteristics Chart
30
Quiz 5
25
Epidemiology Journal
20
Epidemiology Oral Presentation
20
TOTAL POINTS SEMESTER
330
320