Heartland Community College
Fall 2009
Biology 191
Introductory Microbiology Lab
Lab Sections
Lab 1
Lab 2
Lab 3
MW 9:00am – 10:50am
TR 9:00am – 10:50am
MW 1:00pm – 2:50pm
Instructor Information:
Instructor – Leslie Cleary
Phone – 309-268-8514 (HCC)
815-945-7309 (home)
e mail – leslie.cleary@heartland.edu
Office hours – by appointment
Laboratory Manual Information
Students may obtain lab exercise instructions, pre-lab assignments, and final
lab reports by printing instructions from the website at this address:
http://employee.heartland.edu/SROSE01/BIOL191/Lab/lab.index.htm
Notice of Cancelled Class Sessions
Cancelled class sessions, for all HCC classes, will be listed under Cancelled
Class Meetings in the A-Z Index and under Academic Information in the Current
Students page on the HCC Web site. Go to
http://www.heartland.edu/classCancellations/ to learn what classes have been
cancelled for that day and the upcoming week. Be sure to check the last column,
which might contain a message from the instructor.
Microbiology Lab Schedule
Fall 2009
Exercises
Date
Week 1
8/17, 8/18
Introduction to the lab
Video: Bacteria
Lab schedule, safety
regulations, laboratory
equipment and student
evaluation are discussed.
Lab: Microscopy
Principles of microscopy are
introduced. Students learn to
utilize the compound
microscope and immersion oil
techniques.
Day 1
Lab: Widespread Distribution of
Microorganisms.
Students examine the diversity
and ubiquitous nature of
bacteria. Aseptic technique is
introduced.
8/19, 8/20
Week 2
8/24, 8/25
Objectives
Video: The Tree of Life
8/26, 8/27
Week 3
8/31, 9/1
Day 2
Widespread Distribution of
Microorganisms complete.
Students learn simple staining
techniques. Students will
observe the diverse cells using
the microscope.
Day 1
Lab: Pure Cultures and Aseptic
Technique.
Culturing procedures are
performed utilizing a mixed
culture.
QUIZ 1
9/2, 9/3
Day 2
Pure Cultures and Aseptic
Technique continued
Students observe colonies from
the mixed samples and
subculture to isolate pure
cultures.
CD-ROM: Biochemistry: The
Chemistry of Living Things
Students perform interactive
tutorials.
Week 4
9/7, 9/8
9/9, 9/10
LABOR DAY
NO LAB
Day 3
Pure Cultures and Aseptic
Technique complete
Students observe pure cultures
and view them under the
microscope.
Video: E. coli: Confessions of a
Germ
Week 5
9/14, 9/15
9/16, 9/17
Week 6
9/21, 9/22
9/23, 9/24
Day 1
Lab: Sampling Food, Water and
Milk
Sampling procedures are
discussed and performed.
Day 1
Lab: Intestinal Pathogens
Day 2
Sampling Food, Water and Milk
complete
Students will attempt to isolate
intestinal pathogens on selective
media
Colony counts and the
presumptive determination of
contaminating microorganisms
are performed. Students observe
contaminating microbes using
the microscope.
Day 2
Intestinal Pathogens continue
Fermentation tests will be
performed on selected intestinal
pathogens
QUIZ 2
Day 3
Intestinal Pathogens continue
Final inoculations will be
performed on differential media
Day 1
Lab: Control of Microbial
Growth: Alcohol Evaluation &
Effectiveness of Hand Scrubbing
Students begin testing the
effectiveness of alcohol swabs
to kill bacteria and hand
scrubbing to degerm the skin
Day 4
Intestinal Pathogens complete
Final evaluation of isolated
intestinal pathogens
Day 2
Lab: Control of Microbial
Growth: Alcohol Evaluation &
Effectiveness of Hand Scrubbing
complete
Students evaluate the effects of
alcohol and hand scrubbing
Week 7
9/28, 9/29
Day 1
Lab: Control of Microbial
Growth: Evaluation of
Antimicrobics
Students begin tests to
determine the effectiveness of
specific disinfectants,
antiseptics, and antibiotics
Video: Super Staph: Invincible
Bacteria?
9/30, 10/1
Week 8
10/5, 10/6
Day 2
Evaluation of Antimicrobics
complete
Students evaluate the
effectiveness of the
antimicrobics.
Day 1
Lab: Isolation of an Antibiotic
Producer from Soil
Students begin primary
isolation of antibiotic producing
bacteria from soil
Day 2
Isolation of an Antibiotic
Producer from Soil continue
Students will select antibiotic
producing colonies from soil
cultures
Video: Creators of the Future
QUIZ 3
10/7, 10/8
Week 9
10/12, 10/13
10/14, 10/15
Day 3
Isolation of an Antibiotic
Producer from Soil complete
Students will observe soil
cultures for evidence of
antibiosis
Day 1
Lab: Transformation
Students will begin experiments
which will lead to the genetic
transformation of E. coli
CD-ROM: Microbes in Motion
Students will perform
interactive tutorials
Day 2
Transformation complete
Students will observe
transformed bacterial cultures
Lab: DNA Extraction
Students will isolate bacterial
DNA and examine its
characteristics
Week 10
10/19, 10/20
10/21, 10/22
Week 11
10/26, 10/27
10/28, 10/29
Week 12
11/2, 11/3
QUIZ 4
Day 1
Lab: Preparing Unknown
Cultures: Morphological
Characteristics.
Morphological characteristics
of an unknown bacterial culture
are examined.
References needed to determine
the identity of unknowns are
discussed.
Day 2
Preparing Unknown Cultures:
Morphological Characteristics
complete.
Morphological characteristics of
the unknown are examined.
Day 1
Lab: Unknown Cultures:
Physiological Characteristics of
the Unknown
Students test biochemical
characteristics of their
unknown. In addition, students
will become familiar with
problems involved in
determining the identity of an
unknown and beneficial
resource manuals.
Students complete biochemical
testing of their unknown.
References needed to determine
the unknown are utilized.
Day 2
Physiological Characteristics of
the Unknown complete
QUIZ 5
Video: Understanding Viruses
Epidemiology Part 1
11/4, 11/5
Students begin recreating an
investigation involving an
outbreak of a mysterious illness
Unknown Characteristics
Chart due
Week 13
11/9, 11/10
Epidemiology Part 2
Students continue their
investigation.
Epidemiology Part 3
11/11, 11/12
Week 14
11/16, 11/17
Epidemiology Part 4
Students complete recreating an
investigation involving an
outbreak of a mysterious illness
and being preparations for the
oral presentation.
Teams continue preparing for
the oral presentation
Epidemiology Part 5
Oral presentation.
Video: Dangerous Friends and
Friendly Enemies
Epidemiology Journals due
11/18, 11/19
Pre-lab Reports, Lab Reports, and Exams
Excluding the Microscopy, Epidemiology, CD-ROM labs, exercises contain two assignments,
the PRE-REPORT and the LAB REPORT. The Pre-Report should be completed before the day
the exercise BEGINS. It will be collected AT THE BEGINNING of the lab period before the
pre-lab instruction. The purpose of the pre-report is to encourage students to read the lab before
coming to class. It will not be accepted late. The Final Lab Report should be completed and
turned in immediately after the lab is completed. Because lab work is “hands-on”, your presence
in class is necessary. Therefore, if you are absent, the pre-report and/or lab report due that day
will not be accepted. There are no make-up labs.
The laboratory portion of the course comprises 25% of the total BIOL 191 grade. However, a
grade of 70% or higher in the lab portion is required in order to pass the course. Failure to
achieve 70% or more in the lab portion will result in a grade of “F” for the entire course
regardless of the grade in the lecture portion.
Total possible points for the laboratory portion of the course
Microscopy Lab Report
Pre-reports (10 @ 5pt. Each)
Lab reports (11 @ 5pt. Each)
CD-ROM labs (2 @ 5pt. Each)
Quizzes (5 @ 25pt. Each)
Unknown Characteristic Chart
Epidemiology Journal
Epidemiology Oral Presentation
Points
10
50
55
10
125
30
20
20
320 pts.
310 pts.
BIOL 191 Microbiology Lab
Fall 2009
Lab Exercise
Microscopy
Widespread Distribution of Microorganisms
Widespread Distribution of Microorganisms
Biochemistry-CD-ROM
Quiz 1
Pure Culture & Aseptic Technique
Pure Culture & Aseptic Technique
Sampling Food, Water, & Milk
Sampling Food, Water, & Milk
Quiz 2
Intestinal Pathogens
Intestinal Pathogens
Evaluation of Antimicrobics
Evaluation of Antimicrobics
Alcohol Evaluation & Hand Scrubbing
Alcohol Evaluation & Hand Scrubbing
Quiz 3
Antibiotic Production of Soil
Antibiotic Production of Soil
Transformation
Transformation
DNA Extraction
Microbes in Motion-CD-ROM
Quiz 4
Unknown Cultures-Morphological Characteristics
Unknown Cultures-Morphological Characteristics
Physiological Characteristics of the Unknown
Physiological Characteristics of the Unknown
Unknown Characteristics Chart
Quiz 5
Epidemiology Journal
Epidemiology Oral Presentation
TOTAL POINTS SEMESTER
Points
lab report
pre-lab
lab report
pre-lab
lab report
pre-lab
lab report
pre-lab
lab report
pre-lab
lab report
pre-lab
lab report
pre-lab
lab report
pre-lab
lab report
lab report
pre-lab
lab report
pre-lab
lab report
Points possible
10
5
5
5
25
5
5
5
5
25
5
5
5
5
5
5
25
5
5
5
5
5
5
25
5
5
5
5
30
25
20
20
320
310