BIOTECHNOLOGY Midyear Exam Review
UNIT 1 – The Biotechnology Century and its Workforce
1. What is Biotechnology? Definition:
a. List types of biotech:
b. List some biotech jobs:
2. Scientific method: review steps (Bio text, if needed)
UNIT 2 – An Introduction to Genes and Genomes
3. cells
a. review functions of organelles (textbook pp30-31)
b. properties of water: polarity  solvent, osmosis, etc.
c. review plasma membrane – read through bellwork activity before Hepatitis C research
d. CREATE: Venn diagram that compares/contrasts eukaryotes versus prokaryotes (text p28)
PROKARYOTES
EUKARYOTES
4. DNA
a. history of DNA science – review PowerPoint notes and history notes
MATCHING:
1. _____ Miescher
2. _____ Griffith
3. _____ Avery/MacLeod/McCarty
A. demonstrated transformation
B. determined that DNA is genetic
material
C. identified nucleic acids
b. DNA structure – DRAW a nucleotide (text p33)
c. LIST DNA nucleotides: ___________________________________________________________
_______________________________________________________________________________
d. LIST RNA nucleotides: ___________________________________________________________
_______________________________________________________________________________
e. What is a gene? definition:
_______________________________________________________________________________
5. DNA replication – explain:
a. When does this happen? ___________________________________________________________
_______________________________________________________________________________
b. Where? ________________________________________________________________________
_______________________________________________________________________________
c. Why? _________________________________________________________________________
_______________________________________________________________________________
d. What molecules are involved? ______________________________________________________
_______________________________________________________________________________
e. phosphodiester bond - _____________________________________________________________
_______________________________________________________________________________
6. What is a mutation? Definition: _______________________________________________________
_______________________________________________________________________________
a. LIST THREE TYPES OF MUTATIONS (text pp51-53): _________________________________
_______________________________________________________________________________
b. What is a karyotype? Definition: ___________________________________________________
_______________________________________________________________________________
7. protein synthesis – from DNA to protein
a. Central Dogma
b. see notes from 1/6/15
8. What is the epigenome? Definition: ___________________________________________________
_______________________________________________________________________________
UNIT 3 – Recombinant DNA Technology and Genomics
9. recombinant DNA a. What is a restriction enzyme? Where are they found? How are they named?
_________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
b. What makes a good plasmid DNA vector? ____________________________________________
_______________________________________________________________________________
c. What is transformation of bacterial cells? _____________________________________________
_______________________________________________________________________________
10. What is cloning? ___________________________________________________________________
_______________________________________________________________________________
a. DNA libraries? __________________________________________________________________
b. cDNA? ________________________________________________________________________
11. recombinant DNA technology
a. gel electrophoresis – How is it used? _________________________________________________
_______________________________________________________________________________
b. DNA sequencing – How is it used? _________________________________________________
_______________________________________________________________________________
UNIT 4 – Proteins as Products
12. LIST some enzymes (p102/text): ____________________________________________________
_______________________________________________________________________________
13. LIST some protein products (p103/text): ______________________________________________
_______________________________________________________________________________
14. Review structures (p105/text)
15. Production – What is a bioreactor? __________________________________________________
_______________________________________________________________________________
16. COMPLETE TABLE 4.3 (p110/text)
Advantages and Disadvantages of Recombinant Protein Production in E.coli
ADVANTAGES
DISADVANTAGES
17. What is proteomics? Definition: ______________________________________________________
_______________________________________________________________________________