Process of Photosynthesis Photosynthesis Equation Photosynthesis is the process of capturing light energy and converting it into chemical energy, (usually in the form of carbohydrates) 6 CO2 + 6 H2O + Light C6H12O6 + 6O2 Photosynthesis is broken down into two reactions. Light Dependent Reaction energy from light makes the reaction happen Light Independent Reaction (Calvin Cycle; Dark Reaction) Doesn’t need direct light energy, but it does need the high-energy products from the Light Rx. ATP and NADPH Light Dependent ReactionsIn the Chloroplast 1. Light energy is pigments absorbed by _________ in the thylakoid membranes of the chloroplast (pigments are located in Photosystem I & II) 2. In Photosystem II the electrons become energized ________________or “excited”. 3. The excited electrons are passed down a series of molecules called the ____________________ Electron Transport _________. Chain 4. To replace the electrons that are lost, some are stolen from water . This breaks the water molecule apart like this: + 2H2O 4H + O2 5.As electrons move down the chain, their energy is used to ATP (a highmake ______ energy molecule) 6. The electrons now go to Photosystem I. Light energizes new electrons in PI and they combine with NADP+ to make ________ NADPH (another high-energy molecule). LIGHT RX http://www.cnr.vt.edu/DENDRO/forestbiology/photosynthesi s.swf The final products of the light reaction At the end of the light reaction we have made: 1. ATP 2. NADPH 3. O2 Reactants used during the light reaction: 1. Water 2. Also used sunlight Light independent reaction ( Calvin Cycle) A. Needs: CO2 , ATP, NADPH B. Where do they come from? Light Reaction- ATP NADPH Air-CO2 C. Where do they go? Calvin Cycle Location stroma Enzymes are found in the stroma Calvin cycle (dark reaction): doesn’t need light - Uses the ATP and NADPH “charged” by the light reactions to link CO2 together to build C6H12O6 Reactant: 6CO2 Product: C6H12O6 What happens to the glucose? 1. Move to the mitochondria to be converted into ATP through Cell respiration 2. Go through dehydration synthesis to build a big starch chain and be stored for future use Photosynthesis: An Overview Chloroplast Chloroplast Light CO2 H2O NADP+ ADP + P Calvin Cycle LightDependent Reactions ATP NADPH O2 Sugars Factors affecting Photosynthesis: Light Intensity: the rate of increases photosynthesis _________as light intensity_________, increases then levels off CO2 levels: the rate of increases as CO2 photosynthesis _________ increases then levels off _________, What causes the rate to level off in each case? With more light, the plant is limited by the amount of CO2 available. Don’t forget that the wavelength of light matters!!! With more CO2, the plant is limited by the amount light energy available. Temperature: increasing the temperature, _________the increases rate of photosynthesis, then the rate decreases peaks and ____________. Why does the rate decrease after a certain temperature? The enzymes and other molecules controlling the reaction start to break down after that temperature. Alternative Pathways hot Some plants that live in ____, dry environments have ____ modified internal structures and use alternative carbon fixation pathways. water loss Better at preventing _________. Leaf structure Stoma (stomata pl.) How does a plant stop water loss? Close the stomata. But what problem does that cause? Run out of CO2 and too much O2 builds up. C4 pathway The C4 pathway uses a four special ____-carbon compound to “fix” carbon into glucose. This is more efficient when the level of CO2 is low. Examples: corn, crab grass, sugar cane CAM pathways Opens stomata at ________ night to store CO2. Close stomata in the day, which prevents water loss. Examples: pineapple, cacti Exceptions to the Rules: Autotrophic Bacteria: Example: Cyanobacteria (“Blue” bacteria) Exceptions to the Rules: Autotrophic Protists: Example: Algae and Euglena Exceptions to the Rules: Heterotrophic plants: some plants get food from other organisms Mistletoe: makes food AND takes sap (high in sugar) from other trees Exceptions to the Rules: Venus Flytrap: traps and digests insects as a food source Photosynthesis Summary Light Reaction Location Reactants Products Calvin Cycle Photosynthesis Summary Location Reactants Products Light Reaction Thylakoid Calvin Cycle Photosynthesis Summary Location Reactants Products Light Reaction Thylakoid Calvin Cycle Stroma Photosynthesis Summary Location Light Reaction Thylakoid Reactants Light, H2O Products Calvin Cycle Stroma Photosynthesis Summary Location Light Reaction Thylakoid Reactants Light, H2O Products Calvin Cycle Stroma CO2, ATP, NADPH Photosynthesis Summary Location Light Reaction Thylakoid Reactants Light, H2O Products ATP, NADPH, O2 Calvin Cycle Stroma CO2, ATP, NADPH Photosynthesis Summary Location Light Reaction Thylakoid Reactants Light, H2O Calvin Cycle Stroma CO2, ATP, NADPH Products ATP, NADPH, Glucose O2 Photosynthesis: An Overview Chloroplast Chloroplast Light CO2 H2O NADP+ ADP + P Calvin Cycle LightDependent Reactions ATP NADPH O2 Sugars Concept Map Photosynthesis includes Lightdependent reactions Calvin cycle use take place in Energy from sunlight Thylakoid membranes to produce ATP NADPH O2 takes place in Stroma uses ATP NADPH of to produce Chloroplasts High-energy sugars