Chapter 8
Microbial Metabolism
Study guide
What is a metabolic pathway?
Why do cells need enzymes?
Explain enzyme specificity:
Be able to identify parts of an enzyme:
Draw a picture labeling an enzyme. Label: enzyme, substrate, Co-enzyme, enzyme substrate complex, products.
Describe your drawing using complete sentences using the same labels/words to describe as above.
What is an apoenzyme? Describe the components.
Why are coenzymes important?
What are the different factors that affect enzyme activity?
Describe allosteric enzymes.
Describe feedback inhibition.
Compare and contrast competitive and non-competitive inhibitors.
How are catabolic and anabolic reactions different?
Describe the role of ATP in a cell.
What is the difference between oxidative and substrate level phosphorylation?
Describe Oxidation and Reduction:
What is the role of electron carriers in a cell?
Carbohydrate catabolism
Summarize what is happening to glucose when catabolized.
Glycolysis
Answer the following question set for glycolysis:
What is happening to glucose during glycolysis?
How many ATP are used?
How many ATP are generated?
What is the net gain of ATP?
How much and in what form of reducing power is generated?
What are the end products of glycolysis?
Cellular Respiration
What are the 2 types of respiration? How are they different?
Transition reaction
What is the input product into the transition reaction?
How much reducing power and in what form is generated if any?
What are the end products for 1 molecule of glucose?
Krebs cycle
What are the input products for the Krebs cycle?
How much ATP is produced?
How much reducing power and in what form is generated?
What are the end products?
Electron transport system
What is the electron transport system and where is it located?
What is the source of electrons in the electron transport system?
Describe the electron transport system and its role in generating the proton motive force.
What is the proton motive force?
How is ATP synthesized from the proton motive force?
How much ATP is generated from 1 molecule of glucose through oxidative phosphorylation?
What is the difference between aerobic respiration, anaerobic respiration and fermentation?
List some important by-products from fermentation.
Is anaerobic respiration and fermentation the same process?
Describe the catalase test.
Describe the oxidase test.