Eukaryotes • • • • • Protista, Fungi, Plantae, Animalia Complex >10x larger DNA in nucleus Cell membrane – Some also have cell wall • Membrane bound organelles – specialization Protista • Very diverse • mostly unicellular, some colonial, some multicellular (w/simple tissues) • Autotrophs – Plant-like • Heterotrophs – Animal-like protozoans – Fungal-like • Mixotrophs – Photoautotrophic and heterotrophic pathways • Aquatic or moist environments Protistan origin • Eukaryotes that share some Archaea characteristics (e.g. biochemistry & genetics) – Lacks the peptidoglycons in cell walls of bacteria • Otherwise varies in modes of Eukarya reproduction, locomotion, & morphology • Endocytosis; 1o endosymbiosis with prokaryotes (Fig 28.2) – 2o endosymbiosis: heterotroph engulfing red or green algae “…protist phylogeny continues to emerge… changing rapidly…”(Campbell 28.1) • Five Supergroups: – – – – – Excavata Chromalveolata Rhizaria Archaeplastida Unikonta Figure 28.3a Parabasalids Euglenozoans Excavata Diplomonads Apicomplexans Ciliates Diatoms Stramenopiles Golden algae Chromalveolata Alveolates Dinoflagellates Brown algae Oomycetes Forams Radiolarians Green algae Chlorophytes Charophytes Land plants Archaeplastida Red algae Rhizaria Cercozoans Gymnamoebas Entamoebas Opisthokonts Nucleariids Fungi Choanoflagellates Animals Unikonta Amoebozoans Slime molds “…it may be helpful to focus less on the specific names of groups of organisms and more on why the organisms are important…” (Campbell 28.1) Functionally… “Animal-like” Protozoans heterotrophic consumers “Fungi-like” Protists share fungal characteristics; many moved back/forth from Fungi “Plant-like” Algae non-plant, photosynthetic aquatic eukaryotes Diplomonads (Excavata clade – 8 ed.) – Spore forming – Two nuclei – Multiple flagella – Usually anaerobic • Due to their modified mitochondria that lack ETCs & the use of O2 in respiration – E.g. Giardia • Intestinal parasite • Contaminates streams • Causes severe diarrhea Kinetoplastids (Excavata clade – 8 ed.) • Kinetoplast – DNA in mitochondria • • • • E.g. Trypanosoma Blood parasite Single encased flagellum African Tsetse fly – Sleeping sickness (attacks nervous system; lethargy; death if untreated) • So.Amer. Kissing bugs – Chagas disease Amoeboids • Pseudopodia – extensions of the cell • Locomotion • Feeding • Various environments – Aquatic; parasitic; moist soils • Various morphology Protozoans • Animal-like protists – Heterotrophs – Unicellular or colonial – Various forms • Foraminiferans – – – – Have a CaCO3 shell Thin extended pseudopods Planktonic or benthic Adds to the calcareous sediments • Radiolarians – Silica shells; thin pseudopods; planktonic; silicious sediments • Ciliates – Use of cilia to feed and move – Stalked, colonial, or “freeswimming” Ciliates • Cilia to move and feed – E.g. Paramecium, Stentor, Vorticella • Contractile vacuole – osmoregulation • Two nuclei types – Macronucleus • Everyday activities – Micronuclei • Sexual reproduction Apicomplexans • Parasites • Apex structures for penetrating host cells • Lack cilia, flagella, or pseudopods • E.g. Plasmodium – Malaria – Enters and feeds on red blood cells – Vector = ♀ mosquitoes Dinoflagellates • Two flagella in grooves – Spinning flagellates • Phytoplankton – Photosynthesizing aquatic species – Red tides • red pigments along with chl a – Some are toxic • Shell fish accumulation – Bioluminescence Diatoms • Phytoplankton • Silica cell wall – Glass-like – Two halves like a petri plate • Mitotically divide the halves • Secretes the smaller half • Nucleus triggers meiosis when too small Diatom reproduction • Chrysophyta – Silicoflagellates • Star shaped; silicon – Desmids • Pectin & silican walls • Golden or yellow • Coccolithorids – Calcium carbonate coccoliths • All are important planktonic 1o producers Emiliania huxleyi (E. huxleyi) Multicellular Protists (algae) • More photoautotrophic protistans… known as: – Algae, seaweed, kelp – Thalli may be filamentous, grow in mats or crusts, sheets, or kelp • “ Plant-like” primary producers, but not plants: – Lack true leaves, stems & roots • Organized by pigment variations – Phaeophyta (browns) – Rhodophyta (reds) – Chlorophyta (greens) Current phylogeny of the 5 supergroups of Eukaryotes See also: Summary Table on page 598 of text (Ch 28) Phaeophyta • Brown/yellow pigment – Fucxanthin, some phycobilin, some carotenoids (& chl) • Diverse morphologies – Simple, small individual to large & complex (i.e. lengths up to 100m) • kelp forest communities • Some exhibit rapid growth – 1 to 2 feet a day – Important source of algin • Thickener, stabilizer, emulsifier in many products Rhodophyta • Red pigments – Phycoerythrin, Phycobilins, carotenoids • As a group, expands to greater depths than other algae – Why? • Coralline algae – CaCO3 in cell walls • Defense and structure • Important component of coral reef environments – Filamentous or Encrusting • Important component of coral reef environments • Commercial uses – Source of carrageenan & agar (emulsifiers & gel thickeners) • Food – Nori (sushi wraps) from Porphyra Chlorophyta • Green pigments – Chl a & Chl b – Same as plants • Diverse morphologies – – – – Filamentous Sheets Spongy Calcareous • Important component of coral reef environments Other Chlorophytan examples • Colonial • plant-like chloroplasts – Volvox – Ulothrix – Spyrogyra