Revised 3/21/16
Anatomy & Physiology Lab Report Rubric – ELISA
Lab
Avon Grove High School
Mrs. Lamontia & Miss Rurode
1. Title Page (5 pts.)
To get full-credit, you must follow the correct format. The
name of the Lab and the Page number are right-hand justified
starting with the Title page. The following information must
be centered vertically and horizontally (12 pt. Times New
Roman) on a cover sheet and double-spaced:
Name of the Experiment
Name:
Partners’ Names:
Course:
School:
Date Report Completed:
EXAMPLE:
ELISA Lab 1
ELISA Lab
Jane Smith
Sophia Rini, Jorge Ayllon, Frank Schmidt
Anatomy and Physiology
Avon Grove High School
12 March 2009
Revised 3/21/16
2. Introduction (5 pts.)
This should state the purpose of the lab and describe, in
general terms, how the
purpose will be accomplished. It should be about 1/3
to ½ page (100 – 150 words)
long.
3. Expected Results and Underlying Principles (30
pts.)
State the expected results for the positive and negative
controls. Follow this with a brief explanation of why the
presence antibodies in a body fluid sample can be used
to confirm the presence of prior exposure to an antigen.
Include a discussion of the humoral immune response as
well as how the lab uses the events of the humoral
response to detect the antigen. Also explain the purpose
of the Second Antibody and the HRP (enzyme
substrate). Be sure to discuss the real life application of
this test (what test are you simulating).
Do not discuss your results here.
4. Materials (5 pts.)
Briefly list the materials used in the experiment. Do not
include safety glasses, markers, calculators, or routine
measuring devices. Be sure you have the full name of
each solution used, not just an abbreviation.
Revised 3/21/16
5. Procedure (5 pts.)
In a paragraph, briefly describe how the test was
performed. You may use general descriptions such as
“Wash the wells three times with wash buffer.”
6. Data/Results (25 pts.)
Present your results by making a 2- column or 2-row
table with one line per well and list the results as +, - ,
or inconclusive. No conclusions or interpretation of the
data should be included here. Sources of error should be
included in the Discussion.
7. Discussion/Conclusion (15 pts.)
This section is critical to your lab report. Summarize
and explain all results.
Describe possible sources of error, even if your results
were “perfect.” Explain any deviations from expected
results. How could those results have occurred? Explain
uncertainties in observations/measurements.
Conclude with a short paragraph that states the results
for the patient (provide the sample #).
8. Formatting/Grammar/Spelling (10 pts.)
All labs are typed in 12 pt. Times New Roman with 1”
margins. The report should be double-spaced. Avoid
Revised 3/21/16
contractions and colloquial phrases. Write in third
person (no “I”, “we” or “they”). Be sure your verb tense
is consistent and that your antecedents and pronouns
agree. Be sure your verbal clauses modify the correct
noun.
a. GOOD: The pipet was set to 50 microliters then it was
used to transfer 50 microliters of the antigen to each
well.
i. Antecedent- pipet
ii. Pronoun- it
b. BAD: The positive and negative controls was in wells
1-6.
i. Antecedent- positive and negative controls
ii. Pronoun- was
c. Also BAD: A pipet was used to transfer 50 microliters
of the unknown serum from the yellow tube then it
was left untouched for 5 minutes and washed out
i. Antecedent- unsure (could be pipet, yellow tube,
or neither)
ii. Pronoun- it