Cell Organelles and their Function Cell Membrane Cytoplasm •Semi-permeable •Regulates movement in & out •All the material from the nucleus to the cell membrane More on the cell membrane to come… Nucleus Nucleolus Chromosomes Nuclear Envelope •Control center of the cell •Located inside the nucleus •Ribosomes are made here •DNA material seen in the nucleus •Made up of Chromatin (DNA and proteins) •Encloses the nucleus See next slide for chromatin Chromatin DNA wrapped around proteins A protein Chromatin condenses to form the chromosomes Endoplasmic Reticulum Golgi Apparatus Vesicle •Synthesizes and transports proteins (Rough ER) •Synthesizes lipids and steroids (Smooth ER) •Collects, modifies, sorts, and secretes cell products •Picks up molecules from golgi apparatus and transports in cell or to other cells Ribosomes –Synthesize proteins during translation Free – in cytoplasm Bound – on rough ER Lysosome Peroxisome •Break down cellular waste products and debris •Rid cells of hydrogen peroxide and other toxins Mitochondria •Creates energy through cellular respiration Centrosome In animal cells only •Play a role in cell division •Help to organize cytoskeleton •Animal cells have Centrioles which are paired structures within the centrosome •Located near the nucleus Vacuoles • Central Vacuole (in plant cells only) • Food vacuoles • Contractile vacuoles •Holds food, water and minerals •Formed when particles are taken into the cell •Pump water out of cells In Plant Cells Only Plastids – A double membrane bound organelle involved in the synthesis and storage of food, and is commonly found within the cells of photosynthetic organisms, like plants. Chloroplast (example of plastid) Cell Wall Site of photosynthesis in plants only •Thick outer covering outside the cell membrane •Protects and supports a plant cell Cytoskeleton • A network of protein filaments that organizes structures and activities in the cell. • All help to maintain the cell shape and other specific functions – See next slides Components of the Cytoskeleton • Microtubules – Hollow tubes made of proteins known as tubulins – Main Functions • Cell motility (cilia and flagella) • Chromosome movements in cell division • Organelle movements Components of the Cytoskeleton • Microfilaments – Two intertwined strands of actin (a protein) – Main Functions • • • • Changes in cell shape Cytoplasmic streaming Cell motility (pseudopodia) Cell division (cleavage furrow formation) Components of the Cytoskeleton • Intermediate Filaments – Fibrous proteins supercoiled into thicker cables – Main Functions • Anchorage of nucleus and other organelles • Formation of nuclear lamina • The Endomembrane System A cell's endomembrane system consists of the rough and smooth endoplasmic reticulum, Golgi apparatus, vesicles and plasma membrane, all working together to transport cellular materials. http://study.com/academy/lesson/the-endomembrane-system-functions-components.html Cell Membrane • Allows materials to move in and out of the cell. • The “fluid-mosaic model” Of an Animal Cell • Made of a phospholipid bilayer – Two layers of lipids (they can move. They need to be fluid to work properly). Allow certain materials to pass through – dotted with proteins • Proteins aide in certain molecules moving through the membrane Gen bio. Lipids and proteins only. Copy of this slide Phospholipid bilayer of Cell Membrane Water loving • The arrangement of the phospholipid bilayer allows only certain molecules to pass through the cell membrane. • Some large molecules, like sugar Cannot “fit” through this layer • Small molecules generally move through easily • Also if the molecule is soluble in the “tail” region it will go through faster. Water fearing “head region” – likes water “tail region” - does not like water Cell Membrane of an Animal Cell ECM – extracellular matrix (cells attach to this • and send signals to one another. Made of protein fibers) Carbohydrate chains – assist in cell signaling Proteins-aid certain molecules moving through the membrane Cholesterol- keeps membrane fluid, from not freezing or becoming too liquid Regeneration of cells – regrowing finger https://www.youtube.com/watch?v=ITxx2sOLW2Y Phospholipid bilayer – allow certain materials to pass through How do substances move in and out of cells? How Do Substances Move In and Out of Cells? 1. Diffusion – movement of molecules from greater concentration to lesser concentration. No Energy Required by the cell Passive Transport DIFFUSION – A type of Passive Transport How do substances move in and out of cells? 2. Osmosis – movement of water across a membrane from greater to lesser concentration. – – Examples – water into plant cell, salt on slugs, preserving meats Also passive transport (no energy needed) Review of terms • Solute – what is being dissolved • Solvent – What is doing the dissolving • Solution – solute + solvent Types of Solutions • Hypertonic - higher concentration of solute • Hypotonic - lower concentration of solute • Isotonic - equal concentration of solute Less solute / More water More solute/less water Hand out Hand out answer In a solution with higher concentration of solute In a solution with equal concentration of solute and solvent In a solution with lower concentration of solute (more water) • Osmosis and animal cells “Special Cell Processes” Examples Salt on slug E.coli http://www.youtube.com/watch?v=9QCxTf0QfTo&feature=related Osmosis on lettuce https://www.youtube.com/watch?v=SrON0nEEWmo Egg osmosis Facilitated Diffusion Passive Transport No Energy required -Molecules move from Higher concentration to Lower concentration. -Molecules are being “helped” through by proteins The molecules “glide” through, like a boat going through a channel Active Transport -Requires Energy Molecules are now moving the opposite way we have discussed. • Molecules move from a lower concentration to a higher concentration. • Uses a transport protein • Energy comes from ATP Lower concentration https://www.youtube.com/watch?v=ovHYKlHYpyA Review of diffusion and osmosis Higher concentration Active Transport-example • The Sodium (Na), Potassium (K) pump High Na Low K High K Low Na Yahoo search sodium potassium pump - animation Bulk Transport across the cell membrane require energy • Endocytosis – Taking materials into the cell Bulk Transport across the cell membrane require energy • Exocytosis – Secreting materials (moving materials from inside the cell to outside the cell) • Usually moving products