Key: Yellow highlight = required component Human Body Systems Subject Area(s) NS/Cells/ Human Body (homeostasis) Associated Unit Lesson Title Biology/ Chemistry Cellular respiration and Exercise http://www.fuelsvnnanaimo.com/the-importance-of-energy-on-growth/ 1. Synthesis reactions– reactions occur in the body by taking two atoms or molecules and form a larger more complex molecule. A good example of this is when an amino acids synthesize to form a protein molecule. 2. Decomposition reactions– reactions occur in the body that take a larger molecule and break it down into smaller molecules or atoms. A good example is the breakdown of glycogen into glucose. Grade Level 6-12 Lesson # 1 of 1 Time Required 2 hours Summary Throughout the lesson and its activity students are introduced to concepts. These concepts will assist students in investigating the process of respiration. Students will be able to answer the question “How does exercise affect the amount of carbon dioxide exhaled?” All parts of the body require energy so that work can be put in. Muscles need energy to contract and your body may need it to synthesize molecules. Your body receives its energy by combining oxygen and food molecules which is also known as cellular respiration. Sugar glucose in added with oxygen and releases carbon dioxide, CO2, water, H2O, and ATP. ATP is the energy your body can use. Version: August 2013 1 http://gallery4share.com/c/cellular-respiration-reactants-and-products.html Engineering Connection When toxic materials are spilled into the environment, engineers can use microorganisms, fungi or plants to clean up the spill through a process called bioremediation. The engineers choose an organism that can "eat" the target contamination. One way that engineers can tell if the bioremediation is working is by measuring how much the bacteria are "breathing." Engineers measure how much organisms are breathing by changes in pH of the soil or water in which they are growing. Measuring the results of cell activity is usually easier than trying to keep track of the actual amount of toxic material in the environment. Engineering Category = 1. Relating science and/or math concept(s) to engineering 2. Engineering analysis or partial design Keywords cellular respirations, fermentation, oxygen, anaerobic, aerobic respiration, CO2, O2, lactic acid Educational Standards (List 2-4) State STEM Standard (required) SC.912.L.18.8 Identify the reactants, products, and basic functions of aerobic and anaerobic cellular respiration. SC.8.L.18.2 Describe and investigate how cellular respiration breaks down food to provide energy and releases carbon dioxide. SC.912.L.18.9 Explain the interrelated nature of photosynthesis and cellular respiration. Power is the rate at which energy is converted from one form to another or transferred from one place to another, or the rate at which work is done. (6-8) Pre-Requisite Knowledge Version: August 2013 2 Learning Objectives After this lesson, students should be able to: Know why people exercise What happens when you exercise Benefits and consequences of exercise Students will explain the role of the electron transport chain in cellular respiration. Explain why ATP is required for the preparatory steps of glycolysis. State the basic function of fermentation. Compare the fate of pyruvate in alcohol fermentation and in lactic acid fermentation. Compare the processes of fermentation and cellular respiration. Introduction / Motivation Have you ever thought about how your body uses your muscles to exercise? Version: August 2013 3 Aerobic and anaerobic can be simply clarified as the presence and absence of oxygen. Exercise over a long period of time that the oxygen from the lungs is giving it to the muscles. The difference for anaerobic exercise and aerobic exercise is that for an aerobic has to be created from a different source of energy. When engaging in aerobic exercise, muscles contract consecutively without getting tired. However, for anaerobic exercise, muscles cells depend on chemical reactions that do not necessarily need oxygen for muscle contraction. Anaerobic metabolism in cells make waste that can result in damage for muscle contractions. This cause destruction can be recognized as fatigue. However, when exercising one can manage to have a buildup of harmful acids. Lactic acid comes from the lack of oxygen and pyruvic acid. There are three pathways this organism can take; lactic acid fermentation, alcohol fermentation or cellular (anaerobic) respiration. Lesson Background & Concepts for Teachers A glucose is broken down, CO2, is made. If too much CO2 is produced, it can lead up to mutilation to muscles or various body parts. To rid of the CO2, you take in the fresh air and exhale to clear out the stale, CO2 air out of your lungs. A fire needs fuel and oxygen from the air to keep burning. Similarly, your muscles and other parts of your body need a continuous amount of glucose and oxygen to create ATP. Lesson Version: August 2013 4 Materials: 3mL Phenolphthalein solution, straws, 2 plastic pipettes, 2 small beakers, 8mL sodium hydroxide solution, 2 flasks, paper towels, 2 graduated cylinder, timer, 200mL water Lab Preparation 1. Label each plastic pipette – one with a “P” for the phenolphthalein, and one with an “S” for Sodium hydroxide. 2. Label each small beaker – one with a “P” for the phenolphthalein, and one with an “S” for Sodium hydroxide. 3. Label each graduated cylinder - one with a “P” for the phenolphthalein, and one with an “S” for Sodium hydroxide. 4. Label each flask – one as the control, and the other as the variable. 5. Measure out and pour 100 mL of water into each flask. 6. Using the “P” graduated cylinder, measure out 3 mL of phenolphthalein solution into your small beaker labeled “P”. 7. Using the “S” graduated cylinder, measure out 8 mL of sodium hydroxide solution into the small beaker labeled “S”. 8. Using the “P” plastic pipette add 5 drops of phenolphthalein solution to each flask. 9. Using the “S” plastic pipette add 10 drops of sodium hydroxide solution to each flask. Put the rubber stopper on the control flask. Part A – Carbon Dioxide Production Before Exercise 1. Take your resting pulse rate (see “The Pulse” in the Background section). 2. Record your resting pulse rate (before exercise) in Data Table 1. 3. Using the straw, breathe into the variable flask for 30 seconds. MAKE SURE TO REMOVE YOUR MOUTH FROM THE STRAW WHEN YOU NEED TO TAKE A BREATH!!! 4. Notice that the color changes from pink to clear. 5. Using the “S” plastic pipette, add sodium hydroxide solution one drop at a time to the variable flask. COUNT each drop. Continue to add sodium hydroxide solution until the pink color of the variable flask matches the color of the control flask. Record the number of drops it took in Data Table 1 for “Before Exercise”. Part B – Carbon Dioxide Production after Aerobic Exercise 1. Run in place or complete step-ups on a stable chair for 3 minutes. 2. After 3 minutes, take your pulse rate (see “The Pulse” in the Background Section). 3. Record your pulse rate for “Aerobic Exercise” in Data Table 1. Version: August 2013 5 4. Within 30 seconds of stopping exercise, breathe into the variable flask using the straw for 30 seconds. MAKE SURE TO REMOVE YOUR MOUTH FROM THE STRAW WHEN YOU NEED TO TAKE A BREATH!!! If you did not breathe into the straw within 30 seconds of stopping exercise, repeat the 3 minutes of exercise. 5. Using the “S” plastic pipette add sodium hydroxide solution one drop at a time to the variable flask. COUNT each drop. Continue to add sodium hydroxide solution until the pink color of the variable flask matches the color of the control flask. Record the number of drops it took in Data Table 1 for “Aerobic Exercise”. Part C – Carbon Dioxide Production after Anaerobic Exercise 1. Place a textbook in each hand. Hold each textbook out to the sides of your body for one minute or as long as you can. 2. Immediately take your pulse rate (see “The Pulse” in the Background Section). 3. Record your pulse rate for “Anaerobic Exercise” in Data Table 1. 4. Within 30 seconds of stopping exercise, breathe into the variable flask using the straw for 30 seconds. MAKE SURE TO REMOVE YOUR MOUTH FROM THE STRAW WHEN YOU NEED TO TAKE A BREATH!!! If you did not breathe into the straw within 30 seconds of stopping exercise, repeat the exercise. 5. Using the “S” plastic pipette add sodium hydroxide solution one drop at a time to the variable flask. COUNT each drop. Continue to add sodium hydroxide solution until the pink color of the variable flask matches the color of the control flask. Record the number of drops it took in Data Table 1 for “Anaerobic Exercise”. If you have time and your teacher instructs you to do so, repeat Parts A-C for each member of your group. Return any of your extra phenolphthalein solution and sodium hydroxide solution to the original bottle you obtained them from. Data Table 1 Drops of NaOH Pulse Rate Before Exercise Aerobic Exercise Anaerobic Exercise Vocabulary / Definitions Version: August 2013 6 Word Definition Cellular respiration The process of oxidizing food molecules, like glucose, to carbon dioxide and water. The energy released is trapped in the form of ATP for use by all the energy-consuming activities of the cell. The process occurs in two phases: glycolysis, the breakdown of glucose to pyruvic acid. Glucose A sugar with the molecular formula C6H12O6. The name "glucose" means "sweet wine, must.” The suffix "-ose" is a chemical classifier, denoting a carbohydrate. Oxygen A chemical element with symbol O and atomic number 8. It is a member of the chalcogen group on the periodic table and is a highly reactive nonmetallic element and oxidizing agent that readily forms compounds (notably oxides) with most elements. Carbon dioxide A colorless, odorless gas vital to life on Earth. This naturally occurring chemical compound is composed of a carbon atom covalently double bonded to two oxygen atoms. Water A transparent fluid which forms the world's streams, lakes, oceans and rain, and is the major constituent of the fluids of living things. ATP A nucleoside triphosphate used in cells as a coenzyme often called the "molecular unit of currency" of intracellular energy transfer. Anaerobic Exercise An exercise intense enough to trigger lactate formation. It is used by athletes in non-endurance sports to promote strength, speed and power and by body builders to build muscle mass. Aerobic Exercise Physical exercise of low to high intensity that depends primarily on the aerobic energy-generating process. Fermentation A metabolic process that converts sugar to acids, gases or alcohol. It occurs in yeast and bacteria, but also in oxygen-starved muscle cells Lactic Acid An organic compound with the formula CH₃CHCO₂H. It is a white, water-soluble solid or liquid that is produced both naturally and synthetically Associated Activities Have students take theirs and another students’ pulse. Version: August 2013 7 The Pulse: Your pulse is your heart rate, or the number of times your heart beats in one minute. Pulse rates vary from person to person. Lesson Closure Have students reflect back on the lesson, Write a paragraph starting with…. Version: August 2013 8 - I learned…. I wonder how…. This is important because…. This is related to my life….. Assessment Pre-Lesson Assessment Develop a hypothesis or make predictions Scientific Process amd Variables: IV, DV, Control, Constant, Hypothesis IV- different exercise DV- pulse rate, time of solution color change Constant- Rest time, amount of solution mixed Hypothesis- If your___________________, then________________________________. Post-Introduction Assessment Lactic Acid build up in muscles: The build-up of lactic acid comes from when the body breaks down carbohydrates for energy but the oxygen levels are too low. There are a couple ways your body’s oxygen level may drop -During intense exercise -If you have an existing disease or infection A blood test most be performed to measure your lactic acid levels. Have students give examples of intense workouts that can possibly lead to lactic acid build up and preexisting conditions that lead to lactic acid build up. Lesson Summary Assessment Students will answer the following questions to see if the assignment was understood: Version: August 2013 9 a. How do different types of aerobic exercise impact CO2 production? b. How do different types of anaerobic exercise impact CO2 production? c. How do different everyday functions (resting, taking a test, eating, etc.) impact CO2 production? Homework Graph and Analyze Data >Read an exercise and energy summary > Research con(s) of exercising >Research the Disease of Loche wheel > Research lactic acid effects Lesson Extension Activities Review Questions What is cellular respiration? What is fatigue? Give examples of aerobic and anaerobic exercises. What is your resting heart rate? Why does lactic acid build up in muscles? Redirect URL Cellular Respiration and Exercise. (n.d.). Retrieved July 14, 2015, from http://www.haspi.org/curriculum-library/Med-Bio-Core-Labs/Med Bio Labs 2011 - 2013/03 Cellular Respiration & Exercise/03 Cellular Respiration & Exercise - Student.pdf Roth, S. (2006, January 23). Why does lactic acid build up in muscles? And why does it cause soreness? Retrieved July 14, 2015. http://www.scientificamerican.com/article/why-does-lactic-acid-buil/ Contributors Terri Burns-Davis Supporting Program Research Experience for Teachers (RET), Florida International University Engineering Center Acknowledgements Thank you to Dr. Milani Masoud, Stephanie Strange, Lavenette Merus, Brianna Davis Dr. Nezih Pala, and Kerlyn Prada Classroom Testing Information Version: August 2013 10