Microbiology
BIOL 2164 Course Syllabus FALL 2014
Instructor: Jacob Collums
Lecture: Monday & Wednesday 9:30AM-10:50AM
Room 8296
Lab: Monday 11:00AM-1:50AM
Room 8204
Phone: leave message
w/ACS Office
(918) 595-7673
Email: jacob.collums@tulsacc.edu
Office Hrs: by appointment
Associate Dean: Lyn Kent
Science and Mathematics Division Office: 8125
Phone: 918-595-7742
Course Prerequisites
BIO1224 (Intro to Biology for Majors) or equivalent* AND CHE1114 (Principles of Chemistry)
or CHE1315 (General Chemistry).
This course will be taught with the assumption that you have a solid grasp of basic chemical and
biological concepts and terminology. *With approval of SEC Associate Dean of Math and
Science.
Course Description
Included will be a survey of the viruses, protozoa and fungi of medical significance, with an indepth survey of bacteria, their morphology, metabolism, pathogenic capabilities and
classification. Laboratory will emphasize those techniques concerned with isolation and pure
culture of microorganisms and the use of biochemical criteria in taxonomy. Lecture 3 hours.
Laboratory 3 hours. (4 Credit Hours)
Required Textbook and Materials


Microbiology, 4th Edition, Robert W. Bauman, Benjamin Cummings Publishing Co.
A Photographic Atlas for the Microbiology Laboratory 4th Edition, Leboffe and Peirce.
Morton Publishing
 Lab Exercises for Microbiology 2164, TCC Southeast Campus,* Marva Volk, Neil
Enis, Dusti Sloan, Ivan Lurz,; Revised January 2014, TCC Publishing
Labcoat* (disposable), “sunglass style” safety goggles*, slides (1 package)*, non-latex gloves,
colored pencils, permanent marker (fine tip), large 3 ring binder for lab notebook and handouts.
Coverslips will be provided.
*Best to purchase these items from the Southeast Campus bookstore.
SUPPLEMENTAL:
Prentice Hall
USB flash drive (highly recommended);
AIDS Update 2014, Stine,
Course Withdrawal
The deadline to withdraw from a course shall not exceed ¾ the duration of any class. Contact
the Counseling Office at any TCC campus to initiate the withdrawal from a course (“W” grade or
to change from Credit to Audit. Check the TCC Academic Calendar for deadlines. Students
who stop participating in the course and fail to withdraw may receive a course grade of “F”
which may have financial aid consequences for the student. See the information about course
withdrawal on Blackboard. The last day to withdraw with a “W” or change from credit to
audit is Friday, November 7.
Course Objective
 To provide the student with a thorough knowledge of the anatomy and physiology of
microorganisms, with emphasis on the bacteria.
 To indicate bacterial diversity in a survey of the major orders of bacteria and the use of
morphological and physiological differences in establishing taxonomic schemes.
 To survey physical and chemical techniques for microbial control, including sterilization,
disinfection, and chemotherapy.
 To introduce the concept of pathogenesis and the resultant immune response in the
infected host.
 To provide a laboratory experience which will make the student proficient in culturing and
identifying bacteria by using staining, biochemical tests, selective and differential media,
etc.
Computer Assisted Instruction
For any Internet Assignment you may use the Math Lab, Computer Lab, LRC, or you may work
from home if you have internet access.
Disability Resources
It is the policy and practice of Tulsa Community College to create inclusive learning
environments. Accommodations for qualifying students in compliance with the Americans with
Disabilities Act (ADA) and Section 504 of the Rehabilitation Act are available. To request
accommodations, contact the Education Access Center (EAC) at eac@tulsacc.edu or call (918)
595-7115 (Voice). Deaf and hard of hearing students may text (918) 809-1864.
Failure to Withdraw Policy
It is the student’s responsibility to withdraw from the course. Failure to withdraw may result in
the student’s receiving a regular grade of “F” at the end of the semester.
Attendance Policy
Attendance is essential to achieving success. Material presented may not be in your textbook or
difficult to understand. If you must miss a class, it is your responsibility to obtain missed
information from your colleagues, texts, Blackboard, or instructor. More than 3 unexcused
absences may constitute an automatic administrative withdrawal or lowering of one letter of your
final grade.
Institutional Statement
Each student is responsible for being aware of the information contained in the TCC Catalog,
TCC Student Handbook, TCC Student Code of Conduct Policy Handbook, and semester
information listed in the class schedule. All information may be viewed on the TCC website:
www.tulsacc.edu
Learning Support Services
Reading, writing, math and computer learning services are available. Check campus directory
for location and operating hours.
Laboratory Safety
We will be working with live cultures in the laboratory. It is imperative that you follow all
safety recommendations. A lab coat is REQUIRED. You should wash your hands before and
after each lab. Absolutely NO food or drink is allowed in the laboratory. Please inform your
instructor of any aspect of your health status (i.e. pregnancy, medications, convulsions, etc.)
which might impact your lab participation or performance.
Plagiarism Policy
It is the firm policy of your instructors that cheating and plagiarism are unacceptable and will
result in an automatic zero for that assignment or possible failure of the course. Plagiarism is
claiming, indicating, or implying that the ideas, sentences, or words of another writer are your
own; it includes having another writer do work claimed to be your own, copying the work of
another and presenting it as your own, or following the work of another as a guide to ideas or
expression that are then presented as your own. The student should review the relevant sections
of the TCC Student Code of Conduct Policy Handbook.
Academic Dishonesty or Misconduct
Academic dishonesty (cheating) is defined as the deception of others about one’s own work or
about the work of another. Academic dishonesty or misconduct is not condoned or tolerated at
campuses within the Tulsa Community College system. Tulsa Community College adopts a
policy delegating certain forms of authority for disciplinary action to the faculty. Such
disciplinary actions delegated to the faculty include, but are not limited to, the dismissal of
disrespectful or disorderly students from classes. In the case of academic dishonesty a faculty
member may: A. require the student to redo an assignment or test, B. record a “zero” for the
assignment or test in question; C. recommend to the student that the student may withdraw from
the class, or administratively withdraw the student from the class; D. record a grade of “F” for
the student at the end of the semester.
Faculty may request that disciplinary action be taken against a student at the administrative level
by submitting such request to the Dean of Student Services
Computer Services Acceptable Use
Access to computing resources is a privilege granted to all TCC faculty, staff, and students. Use
of TCC computing resources is limited to purposes related to the College’s mission of education,
research, and community service. See TCC Student Code of Conduct Policy Handbook
Cell Phones and other Cellular Devices
Be sure to TURN OFF cell phones and other cellular devices when entering the class.
Inclement Weather
TCC rarely closes. If extreme weather conditions or emergency situations arise, TCC always
gives cancellation notices to radio and television stations. This information is also posted on the
TCC website (www.tulsacc.edu).
General Education Goals
General Education courses at TCC ensure that our graduates gain skills, knowledge, and abilities
that comprise a common foundation for their higher education and a backdrop for their work and
personal lives. TCC’s General Education goals are: Critical Thinking, Effective
Communication, Engaged Learning, and Technological Proficiency.
Classroom Etiquette
Open and mutually respectful communication of varied opinions, beliefs and perspectives during
classroom or online discussion encourages the free exchange of ideas that is essential to higher
learning and to the ability to learn from each other. Use of any electronic device is at the
discretion of the teacher.
Tobacco Free College
Tulsa Community College is a Tobacco Free college in accordance with the Governor’s
Executive Order 2012-01 and Title 63 of the Oklahoma Statutes, Section 1-1523 which prohibits
smoking or the use of any tobacco products in all public places, in any indoor workplace, and all
vehicles owned by the State of Oklahoma and all of its agencies and instrumentalities. This
Order includes property leased, rented, or owned by TCC including, but not limited to, all
grounds, buildings, facilities, and parking lots. Tulsa Community College’s policy includes a
tobacco free environment on all campus and off-campus locations conducting TCC credit or noncredit classes. The TCC Campus Police is responsible for ensuring compliance with the
Tobacco-Free Environment Policy. Violations of the policy may be addressed through issuance
of campus or state citation.
Evaluation
There will be 3 class exams and 1 final exam, as well as, a project and a research paper. Each
laboratory exercise will be graded and included in the total points for the final course grade. You
must inform your instructor PRIOR to laboratories and examinations if you cannot attend.
Make-up exams will most likely be longer, more difficult, and without curve. Contact your
instructor immediately if you miss lab to arrange a make up. Due to the special materials
needed, missed laboratory classes may be made up only with the instructor’s approval. Three or
more absences from lecture, lab and/or failure to complete your Unknown may result in
Administrative Withdrawal from the course. Lab definitions and research questions in italics
and bold print are to be completed BEFORE coming to lab. Lab reports will be checked at
the beginning of lab for pre-lab preparedness and labs reports may be due in lab at the end of the
period the lab is completed. Lab quizzes may be given in lab prior to or following the lab.
Late assignments will not be accepted without instructor approval.
All grades are calculated on a total points system:
-Exams 1-3: 240points (80ea)
-Final: 150points
-Lab Reports: 200points
-Bergey’s Project: 25points
-Lab Final:100points
-Disease Paper: 75points
-Unknown: 50points
Total Points available: 840points
Extra Credit: Disease Presentation, seminars, other
Grading Scale
755.5-840points = A
671.5-755.4points = B
587.5-671.4points = C
503.5-587.4points = D
<503.5points = F
If you have questions about any grade received in this class, such as your grade for a test,
assignment, lab, or report; you must speak with and send a MyTCC email to your instructor
within one week after receiving that grade and work with your instructor to resolve the issue as
soon as possible.
Students are strongly encouraged to maintain an accurate and updated record of progress. It is
strongly recommended you keep up with all work and assignments and ask the instructor for
assistance when needed. Always drop the course if you are failing or unable to attend class.
This is your responsibility to ensure you do not receive an “F” at the end of the semester.
Check with the registrar about the last Drop date.
Research Assignments
Bergey’s Project. 25 Points. This short project is designed to help you become more familiar
with how to use Bergey’s manuals, which you will use to help identify your Unknown. A form
to use for your Bergey’s project and a sign-up sheet will be available in lecture.
Written Disease Report. 75 Points.
Select a bacterial, protozoan, fungal, viral or prion disease. Look in Chapters 19-25 in our text, in
Topley & Wilson’s Microbiology and Microbial Infections (one of the excellent resources
available on reserve in the reference section in our LRC) or in the Bacterial Pathogens,
Mycology, and Parasitic Protozoans Exercises in our lab manual if you need some suggestions
for your topic. Each student must choose a different disease for his or her report. A sign-up
sheet will be available in lecture. The same bacteria may, but does not have to, be used for your
Bergey's project and your disease report. If you choose to do your disease report on a disease not
associated with the bacteria used for your Bergey's project, you may “release it” so that another
student may use that topic for their report.
Review the information on Plagiarism on Blackboard and Handouts before you begin writing.
Write a paper on your topic. Include the classification of your organism in standard format
(“Bergey Project” format for bacteria but no Roman numerals required), in your paper. The text
of your paper EXCLUDING title page information (title, your name, class, date), section
headings, spaces between paragraphs, illustrations, charts and any other materials that you did
not write should be at least five pages double spaced, with 1 inch margins, 12 point font (Times
New Roman, Arial or similar font.) You are allowed 3 lines for your classification information.
Your paper should be in the format of review article and may have a 1/4 - 1/3 page abstract.
You should have five (or more) references, cited in the format used for scientific journals (such
as Nature), for your bibliography.
Your references should include:
1. A full text peer reviewed article, with references, from a refereed journal. For ONE peerreviewed article, turn in a copy of ONLY the title (first) page of the peer-reviewed article
and one page of the bibliography (usually last or next to last page of the paper).
2. One scientific journal article that is at least 2 pages long.
3. A recent journal article (~1 year). NOTE: One article may, but does not have to, fulfill
requirements 1. - 3.
4. An article from the CDC website (see link on Blackboard in Projects&Reports). See me for
an acceptable alternative if needed.
5. Other references may include Topley & Wilson’s Microbiology and Microbial Infections
(available in our LRC) your text (if applicable) and Internet websites.
6. NOTE: You may NOT use Wikipedia as one of you five required references. If you do
use Wikipedia, you must correct the classification scheme to be consistent with Bergey’s
Manual.
You must submit your report to SafeAssignment on Blackboard and receive a plagiarism score of
25% or less. Directions for submitting your paper to SafeAssignment will be posted on
Blackboard. You should post your disease report file on the “Disease Report Parking Spot” in
Reports&Projects on Blackboard for safekeeping.
Turn in a printed copy of your paper, with references, in lecture on the due date. Keep a copy of
your paper to use for your presentation (and as a back-up in case the Lost Assignments monster
eats it.) The paper you turn in will not be returned.
You will have the opportunity to make a ~ 10 minute presentation of your disease report for 10
points extra credit when we cover diseases in lecture. Following the discussion of your disease
presentation, you will present four objective questions to the class and post your questions on our
MyTCC "My Consolidated Courses" site. Exam questions will be selected from student
presentation questions.
Some Recommended Journals (there are many more excellent journals)
Journal of Cell and Molecular Biology, Journal of Microbiology, Science. Virology, Journal of
Infectious Disease, Clinical Infections, Nature, New England Journal of Medicine
-Many of these journals are in the TCC LRCs. Feel free to use journals from other libraries.
Many journal articles are available online. If you use Internet resources, also include the web
address in your bibliography.
Tentative Class Schedule (Note: There may be changes made in the class schedule. Be sure
to keep up with the updates!)
DATE
CHAPTER
LECTURE TOPIC
LABORATORY
August 18
Ch. 4
Microscopy
Lab 1: Microscope &
Bacterial Morphology
August 20
Ch. 1
August 25
Ch. 2
A Brief History of
Microbiology
The Chemistry of
Microbiology
Lab 2 : Aseptic Technique
& Isolation Streak
August 27
Ch. 3
September 1
No Class
Cell Structure and
Function
LABOR DAY 9/1/14
NO LAB
September 3
Ch. 4
September 8
EXAM 1 Ch. 1-4
September 10
Ch. 5
September 15
Ch. 6
September 17
September 22
Staining and
Classification
Lab 3: Gram Stain
Metabolism (omit p. 149158)
Microbial Nutrition and
Growth
Lab 4: Spore Stain & Acid
Fast Stain
Ch. 7
Ch. 7
Microbial Genetics
Transformation
Lab Appendix A-D
Begin Unknown Journal:
Part I. Streak, Ana Jar
September 24
Ch. 8
Recombinant DNA
RNA Interference
(handout, website)
September 29
EXAM 2 Ch. 5-8
October 1
Ch. 9
Controlling Microbial
Growth
October 6
Ch. 10
Antimicrobial Drugs
October 8
Ch.11
Bergey Sign-up
Prokaryotes
Lab 5: Negative Stains:
Nigrosin & Capsule
Begin Lab 6 Selective &
Differential Media
Unknown Journal: Part II.
Unknown Gram Stain,
Catalase & Oxidase tests,
(Morphology)
Complete Lab 6 Selective &
Differential Media
Unknown Morphology
October 13
Ch.12
Eukaryotes
October 15
Ch. 13
Disease Report
Sign-up
Viruses
October 20
Ch. 13
Viruses Cont’d
October 22
EXAM 3 Ch. 9-13
Bergey Project Due
10/22/14
October 27
Ch. 14
Infection & Epidemiology
October 29
Ch. 15
Non-specific Immunity
November 3
Ch. 16
Specific Immunity
November 5
Disease Paper
SafeAssignment
Due 11/5/14
Ch. 17
Immunization
November 10
November 12
November 17
November 19
November 24
November 26
December 1
December 10
Ch. 18
Disease Paper Due
11/12/14
No Class
Immunological Disorders
No Class
Disease
Presentations Ch.1925
Lab Exam Review
No Class
Disease
Presentations Ch.
19-25
Wed. 12/10/14
9-10:50AM
Lab 7: Inoculate
Biochemical Tests; Biochem
Charts Due at
BEGINNING of Lab
Notebook Check: Turn in
Labs 1-6
Lab 7 Read & Turn In
Biochemical Tests
Gloves are REQUIRED for
this lab!
Inoculate broth for Lab 8
(begin working on Lab 8;
prelab due next week)
Lab 8 prelab due. Begin
Lab 8: Effect of UV
Radiation&Antibiotic
Susceptibility Testing
Work on Unknown
Identification in Lab
Begin working on your Lab
9 Charts!!!
Finish & turn in Lab 8 UV
& Antibiotics
Begin Lab 9 Eukaryotic
Microorganisms: Fungi
Last Day to withdraw
Friday November 7
Complete Lab 9
Eukaryotes: Protzoans
Turn in Lab 9 AND Lab 9
Chart
No Lab
Lab Exam Review
Turn in Unknown
Identification
Thanksgiving Break
LAB EXAM
FINAL EXAM
NO LAB