MICROBIOLOGY 20 MORPHOLOGICAL UNKNOWN REPORT
Name: ___________________________
Date: ______________
Unknown Number: __________________
STAINING
PROCEDURE
BROTH
SLANT
SKETCH OR PICTURE
Magnification
Gram Stain
Gram Stain (____X)
Color
Interpretation (+ or -)
Morphology
Arrangement
Acid Fast Stain
Color
Interpretation (+ or -)
Acid Fast Stain (____X)
Capsule Stain
Capsule Stain (____X)
Date:_____________
Interpretation (+ or -)
Endospore stain
Endospore stain (___X)
Date:_____________
Interpretation (+ or -)
Comments:
COLONY CHARACTERISTICS
Diameter (mm)
Pigment Color
Margin
Entire Colony Appearance
Elevation (optional)
Relative Growth (More, less, equal)
BROTH GROWTH: CHECK ALL THAT APPLY
Turbidity
Sediment
Pellicle
Pigment
25 OCELSIUS
37 OCELSIUS
Flocculance
Slant Growth: Describe the growth pattern and color on a slant and draw a sketch below.
Determination of Motility: This unknown is motile (circle answer):
Motility was determined by (circle all that apply):
YES
Hanging drop
Wet mount
NO
Motility agar
+
+
-
-
-
-
-
+
+
+
-
-
-
-
-
-
-
-
-
rod
coccus
rod
rod
rod
rod
rod
single
pairs
single
pairs
single
pairs
single
pairs
37o C
37o C
bluegreen
Red at
<28o C
+
+
Serratia
marcescens
+
Pseudomonas
aeruginosa
Citrobacter
freundii
+
Klebsiella
pneumoniae
Staphylococcus
epidermidis
+
Escherichia
coli
Micrococcus
roseus
+
Mycobacterium
smegmatis
Micrococcus
luteus
Gram
Stain
Endospores
Morphology
Arrangement
Optimal
Temp.
Bacillus
megaterium
+
Bacillus
cereus
Bacillus
subtilis
POSSIBLE UNKNOWN ORGANISMS
rod
rod
rod
coccus
coccus
pairs
chains
pairs
chains
pairs
chains
tetrads
clusters
tetrads
clusters
mycelia
clusters
single
pairs
37o C
30o C
30o C
30o C
25-35o C
37o C
37o C
37o C
37o C
37o C
-
+
+
-
(+ or -)
(+ or -)
+
+
(+ or -)
(+ or -)
Mucoid,
raised,
shiny
colonies
Diffusible
pigment
Fruity odor
Temp.
dependent
pigment
production
Pigment
Motility
Acid
Fast
Capsule
+
+
+
yellow
pink/red
-
-
-
(+ or -)
(+ or -)
(+ or -)
-
-
+
-
Other
“Mousy”
odor
Large
irregular
colonies
Colonies
appear
“wormlike”
after 48 h.
No growth
@52o C
White,
circular,
entire
colonies
FLOWCHART: Construct a flowchart below or on a separate sheet of paper with all possible unknowns listed in the table
and indicate which unknown(s) you have narrowed your specimen down to. NOTE: You are not expected to conclusively
identify your unknown.
Conclusion: Unknown number _________ is (one of) the following organism(s):
_____________________________________________________________________________________
Comments (Indicate if any problems or unexpected results were encountered):