Bio-imagingFacilitypublicationsupto2009

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2009
Milward A, Mankouri J, Harris M. The hepatitis C virus NS5A protein interacts with {beta}catenin and stimulates its transcriptional activity in aPI3K-dependent fashion. J Gen Virol.
2009 Oct 21.
Boyne JR, Whitehouse A. Nucleolar disruption impairs Kaposi'ssarcoma-associated
herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs. FEBS Lett. 2009 Oct
20.
Corless L, Crump C, Griffin SD, Harris M. Vps4 and the ESCRT- III complex arerequired for
the release of infectious hepatitis C virus particles. J Gen Virol. 2009 Oct 14.
Gamlen TP, Richards K, Mankouri J, Hudson L, McCauley J, Harris M, MacdonaldA.
Expression of the NS3 protease of cytopathogenic BVDV results in the inductionof apoptosis
but does not block activation of the interferon beta promoter. J GenVirol. 2009 Sep 30.
Mankouri J, Dallas ML, Hughes ME, Griffin SD , Macdonald A, Peers C, Harris M.
Suppression of a pro-apoptotic K+ channel as a mechanism for hepatitis C viruspersistence.
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15903-8.
Dugan GE, Hewitt EW. Dependence of the localization and function of the human
cytomegalovirus protein US6 on the transporter associated with antigenprocessing. J Gen
Virol. 2009 Sep;90(Pt 9):2234-8.
Jopling HM, Odell AF, Hooper NM, Zachary IC, Walker JH, Ponnambalam S. RabGTPase
regulation of VEGFR2 trafficking and signaling in endothelial cells.Arterioscler Thromb
Vasc Biol. 2009 Jul;29(7):1119-24.
Taneja TK, Mankouri J, Karnik R, Kannan S, Smith AJ, Munsey T, Christesen HB, Beech
DJ, Sivaprasadarao A. Sar1-GTPase-dependent ER exit of KATP channelsrevealed by a
mutation causing congenital hyperinsulinism. Hum Mol Genet. 2009Jul 1;18(13):2400-13.
Hughes M, Griffin S, Harris M. Domain III of NS5A contributes to both RNAreplication and
assembly of hepatitis C virus particles. J Gen Virol. 2009Jun;90(Pt 6):1329-34.
Vohra RS, Walker JH, Howell GJ, Homer-Vanniasinkam S, Ponnambalam S. The LOX1scavenger receptor cytoplasmic domain contains a transplantable endocytic motif. Biochem
Biophys Res Commun. 2009 May 29;383(2):269-74.
Mittar S, Ulyatt C, Howell GJ, Bruns AF, Zachary I, Walker JH, Ponnambalam S. VEGFR1
receptor tyrosine kinase localization to the Golgi apparatus iscalcium-dependent. Exp Cell
Res. 2009 Mar 10;315(5):877-89.
Herbert SP, Odell AF, Ponnambalam S, Walker JH. Activation of cytosolicphospholipase A2{alpha} as a novel mechanism regulating endothelial cell cycleprogression and angiogenesis.
J Biol Chem. 2009 Feb 27;284(9):5784-96.
Emmott E, Hiscox JA. Nucleolar targeting: the hub of the matter. EMBO Rep.2009
Mar;10(3):231-8. Epub 2009 Feb 20. Review.
Walmsley AR , Watt NT, Taylor DR , Perera WS, Hooper NM. alpha-cleavage of theprion
protein occurs in a late compartment of the secretory pathway and isindependent of lipid rafts.
Mol Cell Neurosci. 2009 Feb;40(2):242-8.
2008
Griffin S, Trowbridge R, Thommes P, Parry N, Rowlands D, Harris M, Bright H. Chimeric
GB virus B genomes containing hepatitis C virus p7 are infectious in vivo. J Hepatol. 2008
Sep 15. [Epub ahead of print]
Griffin S, Stgelais C, Owsianka AM, Patel AH, Rowlands D, Harris M. Genotype-dependent
sensitivity of hepatitis C virus to inhibitors of the p7 ion channel. Hepatology. 2008 Jul 28.
[Epub ahead of print]
Lambert LA, Whyteside AR, Turner AJ, Usmani BA. Isoforms of endothelin-converting
enzyme-1 (ECE-1) have opposing effects on prostate cancer cell invasion. Br J Cancer. 2008
Oct 7;99(7):1114-20. Epub 2008 Sep 9.
Emmott E, Dove BK, Howell G, Chappell LA, Reed ML, Boyne JR, You JH, Brooks G,
Whitehouse A, Hiscox JA. Viral nucleolar localisation signals determine dynamic trafficking
within the nucleolus. Virology. 2008 Sep 3.
Peckham M. Engineering a multi-nucleated myotube, the role of the actin cytoskeleton. J
Microsc. 2008 Sep;231(3):486-93.
Xia R, Mei ZZ, Milligan C, Jiang LH. Inhibitory interaction between P2X4 and GABA(C)
rho1 receptors. Biochem Biophys Res Commun. 2008 Oct 10;375(1):38-43. Epub 2008 Jul
31.
Smith AJ, Sivaprasadarao A. Investigation of K(ATP) channel endocytosis by
immunofluorescence. Methods Mol Biol. 2008;491:69-77
Mankouri J, Milward A, Pryde KR, Warter L, Martin A, Harris M. A comparative cell
biological analysis reveals only limited functional homology between the NS5A proteins of
hepatitis C virus and GB virus B. J Gen Virol. 2008 Aug;89(Pt 8):1911-20.
Foresti O, Denecke J. Intermediate organelles of the plant secretory pathway: identity and
function. Traffic. 2008 Sep;9(10):1599-612. Epub 2008 Jul 9.
Murphy JE, Vohra RS, Dunn S, Holloway ZG, Monaco AP, Homer-Vanniasinkam S, Walker
JH, Ponnambalam S. Oxidised LDL internalisation by the LOX-1 scavenger receptor is
dependent on a novel cytoplasmic motif and is regulated by dynamin-2. J Cell Sci. 2008 Jul
1;121(Pt 13):2136-47. Epub 2008 Jun 10.
You JH, Howell G, Pattnaik AK, Osorio FA, Hiscox JA. A model for the dynamic
nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory
syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging. Virology. 2008
Aug 15;378(1):34-47. Epub 2008 Jun 12.
Mankouri J, Griffin S, Harris M. The hepatitis C virus non-structural protein NS5A alters the
trafficking profile of the epidermal growth factor receptor. Traffic. 2008 Sep;9(9):1497-509.
Epub 2008 Jun 9.
Wilshaw SP, Burke D, Fisher J, Ingham E. Investigation of the antiadhesive properties of
human mesothelial cells cultured in vitro on implantable surgical materials. J Biomed Mater
Res B Appl Biomater. 2008 Jun 10. [Epub ahead of print]
Conway CL, Walker I, Bell A, Roberts DJ, Brown SB, Vernon DI. In vivo and in vitro
characterisation of a protoporphyrin IX-cyclic RGD peptide conjugate for use in
photodynamic therapy. Photochem Photobiol Sci. 2008 Mar;7(3):290-8.
Dunn S, Morrison EE, Liverpool TB, Molina-París C, Cross RA, Alonso MC, Peckham M.
(2008) Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live
cells. J Cell Sci. 2008 121(Pt 7):1085-95.
Bahnasi YM, Wright HM, Milligan CJ, Dedman AM, Zeng F, Hopkins PM, Bateson AN,
Beech DJ.(2008) Modulation of TRPC5 cation channels by halothane, chloroform and
propofol. Br J Pharmacol. 153:1505-12.
Xu SZ, Sukumar P, Zeng F, Li J, Jairaman A, English A, Naylor J, Ciurtin C, Majeed Y,
Milligan CJ, Bahnasi YM, Al-Shawaf E, Porter KE, Jiang LH, Emery P, Sivaprasadarao A,
Beech DJ. TRPC channel activation by extracellular thioredoxin. Nature. 451(7174):69-72.
2007
You JH, Reed ML, Hiscox JA. (2007) Trafficking motifs in the SARS-coronavirus
nucleocapsid protein. Biochem Biophys Res Commun. 358:1015-20. Pubmed
Cawood R, Harrison SM, Dove BK, Reed ML, Hiscox JA. (2007) Cell cycle dependent
nucleolar localization of the coronavirus nucleocapsid protein. Cell Cycle. 6:863-7. Pubmed
Taylor DR, Hooper NM. (2007) The low-density lipoprotein receptor-related protein 1
(LRP1) mediates the endocytosis of the cellular prion protein. Biochem J. 402:17-23. Pubmed
Harrison SM, Dove BK, Rothwell L, Kaiser P, Tarpey I, Brooks G, Hiscox JA. (2007)
Characterisation of cyclin D1 down-regulation in coronavirus infected cells. FEBS Lett.
[Epub ahead of print] Pubmed
Hiscox JA. (2007) RNA viruses: hijacking the dynamic nucleolus. Nat Rev Microbiol. 5:11927 Pubmed
Reed ML, Howell G, Harrison SM, Spencer KA, Hiscox JA. (2007) Characterisation of the
nuclear export signal in the coronavirus infectious bronchitis virus nucleocapsid protein. J
Virol. Pubmed
2006
Foresti O, daSilva LL, Denecke J. (2006) Overexpression of the Arabidopsis syntaxin
PEP12/SYP21 inhibits transport from the prevacuolar compartment to the lytic vacuole in
vivo. Plant Cell. 18:2275-93.
Musa H, Meek S, Gautel M, Peddie D, Smith AJ, Peckham M. (2006) Targeted homozygous
deletion of M-band titin in cardiomyocytes prevents sarcomere formation. J Cell Sci.
119:4322-31
Boyne JR, Whitehouse A. (2006) Nucleolar trafficking is essential for nuclear export of
intronless herpesvirus mRNA. Proc Natl Acad Sci USA . 103:15190-5.
Boyne JR, Whitehouse A. (2006) gamma-2 Herpes virus post-transcriptional gene regulation.
Clin Microbiol Infect. 12:110-7.
Griffiths RA, Boyne JR, Whitehouse A. (2006) Herpesvirus saimiri-based gene delivery
vectors. Curr Gene Ther. 6:1-15.
Swailes NT , Colegrave M, Knight PJ, Peckham M. (2006) Non-muscle myosins 2A and 2B
drive changes in cell morphology that occur as myoblasts align and fuse. J Cell Sci.
119:3561-70. Dalton JE , Cruickshank SM , Egan CE , Mears R , Newton DJ , Andrew EM ,
Lawrence B , Howell G , Else KJ , Gubbels MJ , Striepen B , Smith JE , White SJ , Carding
SR . (2006) Intraepithelial gammadelta(+) Lymphocytes Maintain the Integrity of Intestinal
Epithelial Tight Junctions in Response to Infection. Gastroenterology 131: 818-29. Pubmed
Smith AJ, Partridge CJ, Asipu A, Mair LA, Hunter M, Sivaprasadarao A (2006). Increased
ATP-sensitive K + channel expression during acute glucose deprivation. Biochem. Biophys.
Res.Comm. (In Press)
Mankouri J, Taneja TK, Smith AJ, Ponnambalam S, Sivaprasadarao A (2006) Kir6.2
mutations causing neonatal diabetes prevent endocytosis of ATP-sensitive potassium
channels. EMBO J (in press)
Ewan LC, Jopling HM, Jia H, Mittar S, Bagherzadeh A, Howell GJ, Walker JH, Zachary IC,
Ponnambalam S. (2006) Intrinsic Tyrosine Kinase Activity for Vascular Endothelial Growth
Factor Receptor 2 Ubiquitination, Sorting and Degradation in Endothelial Cells. Traffic
7:1270-1282
Reed ML, Dove BK, Jackson RM, Collins R, Brooks G, Hiscox JA (2006) Delineation and
modelling of a nucleolar retention signal in the coronavirus nucleocapsid protein. Traffic 7:116.
Street M, Marsh SJ, Stabach PR, Morrow JS, Brown DA, Buckley NJ. (2006) Stimulation of
Galphaq-coupled M1 muscarinic receptor causes reversible spectrin redistribution mediated
by PLC, PKC and ROCK. J Cell Sci. 119:1528-36
Dove BK, Brooks G, Bicknell K, Wurm T, Hiscox JH. (2006) Cell cycle perturbations
induced by infection with the coronavirus infectious bronchitis virus and their effect on virus
Replication. J Virology 80:4147-56. Pubmed
Dove BK, You J, Reed ML, Emmett SR, Brooks G, Hiscox JH. (2006) Changes in nucleolar
morphology and proteins during infection with the coronavirus infectious bronchitis virus.
Cellular Microbiology. In press
Brooke RE, Atkinson L, Edwards I, Parson SH, Deuchars J. (2006) Immunohistochemical
localisation of the voltage gated pottasium ion channel subunit Kv3.3 in the rat medulla
oblongata and thoracic spinal cord. Brain Res. 1070:101-115. Pubmed
McCormick CJ, Maucourant S, Griffin S, Rowlands DJ, Harris M. (2006) Tagging of NS5A
expressed from a functional hepatitis C virus replicon. J Gen Virol 87:635-640. Pubmed
Bentham M, Mazaleyrat S, Harris M. (2006) Role of myristoylation and N-terminal residues
in membrane association of the human innumodeficiency virus type 1 Nef protein. J Gen
Virol 87:563-571. Pubmed
Murphy JE, Tacon D, Tedbury PR, Hadden JM, Knowling S, Sawamura T, Peckham M,
Phillips SE, Walker JH, Ponnambalam S. (2006) LOX-1 scavenger receptor mediates
calcium-dependent recognition of phosphatidylserine and apoptotic cells. Biochem J . 393
:107-15. Pubmed
2005
You J, Dove BK, Enjuanes L, DeDiego ML, Alvarez E, Howell GJ, Heinen P, Zambon M,
Hiscox JA (2005) Subcellular localization of the severe acute respiratory syndrome
coronavirus nucleocapsid protein. J Gen Virol 86:3303-3310. Abstract
Awano S, Dawson LA , Hunter AR, Turner AJ, Usmani BA. (2005) Endothelin system in oral
squamous carcinoma cells: Specific siRNA targeting of ECE-1 blocks cell proliferation. Int J
Cancer [Epub ahead of print] Pubmed
Griffin S , Clarke D , McCormick C , Rowlands D , Harris M . (2005) Signal peptide cleavage
and internal targeting signals direct the hepatitis C virus p7 protein to distinct intracellular
membranes. J Virol 79 :15525 Abstract
Taylor DR, Watt NT, Perera WS, Hooper NM. (2005) Assigning functions to distinct regions
of the N-terminus of the prion protein that are involved in its copper-stimulated, clathrindependent endocytosis. J Cell Sci 118 :5141-53. Pubmed Abstract
Warner FJ, Lew RA, Smith AI, Lambert DW, Hooper NM, Turner AJ. (2005) Angiotensinconverting enzyme 2(ACE2), but not ACE, is preferentially localized to the apical surface of
polarised kidney cells. J Biol Chem Sep 15; [Epub ahead of print] Pubmed
Baxter RL, Vega-Riveroll LJ, Deuchars J, Parson SH. (2005) A2A adenosine receptors are
located on presynaptic motor nerve terminals in the mouse. Synapse 15:229-34. Pubmed
Herbert SP, Ponnambalam S, Walker JH. (2005) Cytosolic phospholipase A2-alpha mediates
endothelial cell proliferation and is inactivated by association with the Golgi apparatus. Mol
Biol Cell 16: 3800-9. Pubmed
Barnes K, McIntosh E, Whetton AD, Daley GQ, Bentley J, Baldwin SA. (2005) Chronic
myeloid leukaemia: an investigation into the role of Bcr-Abl-induced abnormalities in glucose
transport regulation. Oncogene 24: 3257-67. Pubmed
Moores TS, Hasdemir B, Vega-Riveroll L, Deuchars J, Parson SH. (2005) Properties of
presynaptic P2X7-like receptors at the neuromuscular junction. Brain Res 1034: 40-50.
Baldwin SA, Yao SY, Hyde RJ, Ng AM, Foppolo S, Barnes K, Ritzel MW, Cass CE, Young
JD. (2005) Functional characterization of novel human and mouse equilibrative nucleoside
transporters (hENT3 and mENT3) located in intracellular membranes. J Biol Chem 280:
15880-7
daSilva LLP, Taylor JP, Hadlington JL, Hanton SL, Snowden CJ, Fox SJ, Foresti O,
Brandizzi F, Denecke J. (2005) Receptor salvage from the prevacuolar compartment is
essential for efficient vacuolar protein targeting. The Plant Cell 17:132
Henderson Z, Boros A, Jenzso G, Westwood AJ, Monyer H, Halasy K. (2005) Somatodendritic nicotinic responses recorded in vitro from the medial septal diagonal band complex
of the rodent. J Physiology 562:165-182
2004
Henderson Z, Fiddler G, Saha S, Boros A, Halasy K. (2004) A parvalbumin-containg,
axosomatic synaptic network in the rat medial septum: relevance to rhythmogenesis.
European Journal Neuroscience 19:2753-2768
Howell GJ, Herbert SP, Smith JM, Mittar S, Ewan LC, Mohammed M, Hunter AR, Simpson
N, Turner AJ, Zachary I, Walker JH, Ponnambalam S. (2004) Endothelial cell confluence
regulates Weibel-Palade body formation. Mol Memb Biol 21:413
Dalton JE, Howell G, Pearson J, Scott P, Carding SR. (2004) Fas-Fas ligand interactions are
essential for the binding to and killing of activated macrophages by gamma delta T cells. J
Immunol 173:3660-3667
Tacon D, Knight PJ, Peckham M. (2004) Imaging myosin 10 in cells. Biochem Soc Trans 32:
689-93
Fox EJ, Stubbs SA, Kyaw Tun J, Leek JP, Markham AF, Wright SC. (2004) PRELI (protein
of relevant evolutionary and lymphoid interest) is located within an evolutionarily conserved
gene cluster on chromosome 5q34-q35 and encodes a novel mitochondrial protein. Biochem J
378: 817-25.
Pang S, Urquhart P, Hooper NM. (2004) N-Glycans, not the GPI anchor, mediate the apical
targeting of a naturally glycosylated, GPI-anchored protein in polarised epithelial cells. J Cell
Sci 117 :5079-5086
Dawson LA , Maitland NJ, Turner AJ, Usmani BA. (2004) Stromal-epithelial interactions
influence prostate cancer cell invasion by altering the balance of metallopeptidase expression.
Br J Cancer . 90 :1577-82.
Barnes K, Ingram JC, Bennett MD, Stewart GW, Baldwin SA. (2004) Methyl-betacyclodextrin stimulates glucose uptake in Clone 9 cells: a possible role for lipid rafts.
Biochem J . 378 :343-51.
Griffin SD , Harvey R, Clarke DS, Barclay WS, Harris M, Rowlands DJ. (2004) A conserved
basic loop in hepatitis C virus p7 protein is required for amantadine-sensitive ion channel
activity in mammalian cells but is dispensable for localization to mitochondria. J Gen Virol .
85 :451-61
2003
Musa H, Orton C, Morrison EE, Peckham M. (2003) Microtubule assembly in cultured
myoblasts and myotubes following nocodazole induced microtubule depolymerisation. J
Muscle Res Cell Motil . 24: 301-8.
Brooks DR , Appleford PJ, Murray L, Isaac RE. (2003) An essential role in molting and
morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensinconverting enzyme family that lacks a metallopeptidase active site. J Biol Chem . 278 :523406
Bentley J, Itchayanan D, Barnes K, McIntosh E, Tang X, Downes CP, Holman GD, Whetton
AD, Owen-Lynch PJ, Baldwin SA. (2003) Interleukin-3-mediated cell survival signals
include phosphatidylinositol 3-kinase-dependent translocation of the glucose transporter
GLUT1 to the cell surface. J Biol Chem. 278 :39337-48
Miller G, Musa H, Gautel M, Peckham M. (2003) A targeted deletion of the C-terminal end
of titin, including the titin kinase domain, impairs myofibrillogenesis . J Cell Sci . 116 :48119
Parkin ET, Tan F, Skidgel RA, Turner AJ, Hooper NM. (2003) The ectodomain shedding of
angiotensin-converting enzyme is independent of its localisation in lipid rafts. J Cell Sci. 116
:3079-87
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