Name: _________________________________________________ Period: _____________
2.5
Enzyme Review Sheet
1.
The diagram below illustrates a biochemical process that occurs in organisms.
Simple Sugar
Simple Sugar
+ WATER ->
+
Catalyst
Catalyst
The substance labeled “catalyst” is also known as
a. A hormone
b. An enzyme
2.
c. An antibody
d. An inorganic compound
Is this an anabolic or catabolic reaction? _______________________________
An investigation was preformed to determine the effects of enzyme X on their different disaccharides
at 37oC. Three test tubes where set up as shown in the diagram below.
5 milliliters
sugar B solution
= 1 milliliter
enzyme X
5 milliliters
sugar A solution
= 1 milliliter
enzyme X
5 milliliters
sugar C solution
= 1 milliliter
enzyme X
At the end of 5 minutes, the solution in each test tube was tested for the presences of disaccharides
and monosaccharides. The results of these tests are shown in the table below.
Test tube 1
Not present
Present
Monosaccharide
Disaccharide
Test tube 2
Not present
Present
Test tube 3
Present
Not present
3. What can be concluded about the activity of enzyme X from the data table? _______________
___________________________________________________________________________________________
4.
A characteristic of hormones and enzymes that allows them to work effectively with other
organic molecules is their
a. Specific shape
b. Small size
c. Concentration of carbon and hydrogen atoms
d. High-energy bonds
Rate of enzyme action
5. The effect of pH on a certain enzyme is shown in the graph below.
0
2
4
6
8
10
12
At what pH would the enzyme be most effective?
a. Above 10
b. Between 5 and 7
14
pH
c. Between 8 and 10
d. Below 5
Base your answers to questions 5 through 7 o the diagram below that represents a human
enzyme and four types of molecules present in a solution in a flask.
A
C
B
Enzyme
D
6. Which molecule would most likely react with the enzyme? _____________
7. Explain your answer to question 5. __________________________________________________
___________________________________________________________________________________
8. State what would most likely happen to the rate of reaction if the temperature of the
solution in the flask were increased gradually from 10oC to 30oC. _____________________
___________________________________________________________________________________
___________________________________________________________________________________
Rate of enzyme action
9. A colony of bacteria growing on a culture medium is successfully synthesizing an
organic compound. Which procedure would be least likely to have an effect on this
synthesis?
a. Adding more subunits of the organic compound to the medium
b. Lowering the pH of the medium
c. Raising the temperature of the colony from 20oC to 30oC
d. Increasing the number of hormone molecules in the colony.
Trypsin
Pepsin
0
2
4
6
8
10
12
10. Pepsin works best in which type of environment?
a. Acidic, only
b. Basic, only
c. Neutral
d. Sometimes acidic, sometimes basic
11. Neither enzyme above works at a pH of
a. 1
b. 6
c. 3
d. 13
14
pH
The diagram below represents a beaker containing a solution of various molecules involved
in digestion.
D
A
E
B
C
Enzyme Activity
12. Which structures represent products of digestion?
a. A and D
b. B and E
c. B and C
d. D and E
13. An incomplete graph is shown below
Effect of Z on Enzyme Activity
Z
What label would appropriately be used to replace letter Z on the axis? ____________________
14. Enzymes have an optimum temperature at which they work best. Temperatures
above and below this optimum will decrease enzyme activity. Which graph best
illustrates the effect of temperature on enzyme activity?
15. An enzyme and four different molecules are shown in the diagram below.
Enzyme
A
B
C
The enzyme would most likely affect reactions involving
a.
b.
c.
d.
Molecule A only
Molecule C only
Molecules B and D
Molecules A and C
16. Define the term denatured.
17. Define the term active site.
18. Define the term substrate.
D
19. Enzyme molecules are affected by changes in conditions within organisms. Explain
how a prolonged, excessively high body temperature during an illness could be fatal
to humans. Your answer must include:
i. The role of enzymes in a human
ii. The effect of this high body temperature on enzyme activity
iii. The reason this high body temperature can result in death
20. Correctly complete the following chart.
Organic Compound Class
Subunit or building block of
compound
Fatty acids and glycerol
Carbohydrates
Nucleotides
Function(s) or example(s) of
the compound
Antibodies, enzymes, some
hormones--- the function of
the compound is controlled
by its shape
Energy storage, cushioning
and insulation
Includes starches and sugars
which are used as energy
sources and cellulose which
makes up cell walls in plants
Has the genetic code in the
nucleus and is involved in
protein synthesis at the
ribosome; DNA and RNA are
examples