Name Period PLANT AND ANIMAL CELLS -A Lab Exercise Comparing Cell Types Objectives: To observe, identify, and distinguish between plant and animal cells and their organelles. Materials: Dropper, beaker for water, 2 slides, tweezers, Elodea plant, cover slips, microscope, flat toothpick, iodine PART I: Elodea Plant Procedure 1. Place a drop of water in the center of a clean slide to prepare a wet mount specimen. 2. Use tweezers to remove a small leaf from the Elodea plant. You only need ONE leaf. Place the leaf, BOTTOM SIDE UP, in the drop of water. Place a cover slip over the leaf. 3. Observe the Elodea leaf through the microscope under low power and then under high power. 4. Using high power, sketch 2-3 cells and label: CELL WALL, CHLOROPLASTS, NUCLEUS, CELL MEMBRANE, CYTOPLASM Part I: Elodea Analysis Questions 1. There are some tiny green circles within each Elodea cell. What are they called? ______________ 2. What is the function of the green circles? 3. What is the shape of the Elodea cell? _____________________________________ 4. Which part of the plant cell gives it shape?_________________________________ 5. What organelles do Elodea (and all other plant cells) have that animal cells don’t? PART II: Onion Cell Procedure Onion Cells 1. Use tweezers to remove a small, thin piece of the onion skin. 2. Place one drop of methylene blue on the onion skin – make sure it stains the onion. Add a coverslip (Make sure you can put the slip cover on the sample and it still lay FLAT.) 3. Observe the Elodea leaf through the microscope under low power and then under high power. 4. Using high power, sketch 2-3 cells and label: CELL WALL, NUCLEUS, CELL MEMBRANE, CYTOPLASM Part II: Onion Analysis Questions 1. What kind of cell in the onion cell? _____________________________ What structure is missing from the onion cell? _____________Why? _____________________________________________________________________________ 1 PART III: Animal Cheek (Epithelial) Cells Procedure 1. Using the blunt end of a toothpick, gently scrape some cells from the inside of your cheek. 2. Make a wet mount by smearing the cheek cells onto a slide containing ONE drop of IODINE and covering it with a cover slip. 3. Observe the cheek cells using the microscope under low power and then under high power. 4. Using high power, sketch 2-3 cells and label: CELL MEMBRANE, NUCLEUS, CYTOPLASM. Part III: Animal Cheek Cell Analysis Questions 1. How does the shape of the cheek cell differ from the Elodea? 2. When you added the iodine to the cheek cell, an organelle became visible as a small darker gold circle in the middle of the cell. What do you think this gold circle is? ______________ 3. Are the cheek cells the same or do they vary in shape? 4. Why are there no chloroplasts in the cheek cells? 5. Which part of the animal cell gives it shape?_________________________________ CONCLUSION: Are any of the cells observed prokaryotic? ______ Explain why or why not. ______________________________________________________________ ___________________________________________________________________________________ 1. A nucleus can be found in (circle one) PLANT ANIMAL BOTH 2. A cell membrane can be found in (circle one) PLANT ANIMAL BOTH 3. A cell wall can be found in (circle one) PLANT ANIMAL BOTH 4. Cytoplasm can be found in (circle one) PLANT ANIMAL BOTH 5. Ribosomes can be found in (circle one) PLANT ANIMAL BOTH 6. Chloroplast can be found in (circle one) PLANT ANIMAL BOTH 7. Mitochondria can be found in (circle one) PLANT ANIMAL BOTH 2