CELLS Structures and Functions Basic Parts of All Cells 1. Plasma membrane – barrier between in and outside of cell 2. Cytoplasm – region inside plasma membrane • Cytosol – includes molecules and small particles but not organelles 3. Genetic material 4. Ribosomes Organelles! Eukaryotic Cell Nucleus The Nucleus 1. Most obvious structure (5μm) 2. Most eukaryotic cells contain a nucleus 3. Function: • • Controls most functions of cell Houses and protects genetic information 4. Structure: • • • Nuclear envelope – double membrane layer contains genetic contents; porous – materials enter and exit Nucleoplasm – jelly like fluid filling nucleus Nucleolus – dense area where ribosomes are made 5. Processes: • Cell Division When cell is not dividing - DNA exists as chromatin: long, thin structures When cell is about to divide - DNA condenses to form chromosomes: structures made of DNA and protein • Transcription – DNA converted to RNA in the nucleus Chromatin Cell Division Chromosome Mitochondria Mitochondria 1. Size: 1-10 μm long 2. Function: • Transfer energy from organic molecules to adenosine triphosphate (ATP) – powers most cellular reactions 3. Structure: inner & outer membrane • Outer – separates mitochondria from cytosol • Inner – many folds (cristae) that capture and store energy • Mitochondrial DNA – have own DNA • THEORY: mitochondria originated from prokaryotic cells 4. Processes: • Cellular respiration – enzymes in matrix aid in formation of ATP by extracting energy from food sources Ribosomes Ribosomes 1. small, spherical (< 25 nanometers) 2. Function: • Build proteins • Free ribosomes – make proteins used in cytosol (Ex: enzyme) • Bound ribosomes – make proteins used in membranes, packaging, and elsewhere in body 3. Structure: • Manufactured partly in nucleolus (protein & RNA), completed in cytoplasm • Have small and large subunit Endoplasmic Reticulum Endoplasmic Reticulum (ER) 1. 2. 3. System of cisternae - membranous tubes & sacs Continuous with nuclear membrane Function – moves molecules from one part of the cell to another 4. Structure – Rough and Smooth 1. Rough ER – covered with ribosomes; involved in making proteins a. Secretory proteins – secreted in vesicles (Ex: digestive enzymes) b. Membrane proteins – inserted in membranes 2. Smooth ER – no ribosomes; builds lipids, produces steroid hormones, detoxifies drugs and poisons Golgi Apparatus Golgi Apparatus 1. 2. System of cisternae stacked up Function: a. Manufacturing, warehousing, sorting, shipping b. Work with nearby vesicles coming from ER – vesicle contents modified, given “address label (carbohydrate label),” transported to other parts of cell 3. Structure: a. Distinct polarity – membranes on opposite ends have different thickness & molecular composition b. Two poles: 1. Cis (same side) – receives materials from ER 2. Trans (opposing side) – vesicles bud off & are transported/exported Vesicles 1. Small, spherical shaped sacs 2. Function: a. Migrate to and merge with cell membrane b. Release contents outside of cell 3. Structure: a. Single membrane b. Different types (lysosomes, peroxisomes) Lysosomes 1. Structure – bud from Golgi, contain digestive enzymes – each vesicle is specific! 2. Function – Break down macromolecules (proteins, carbohydrates, nucleic acids) 3. Processes: 1. Autophagy – Digestion of worn-out organelles 2. Autolysis – Digestion of damaged or extra cells when time for cell to die 3. Phagocytosis – Cell eating 4. Receptor-mediated endocytosis Vesicles Peroxisomes 1. NOT produced in Golgi, contain different enzymes, such as Catalase 2. Enzymes involved in reactions 3. Different types: Ex: Detoxify alcohol and other drugs 4. Break down fatty acids Vacuole 1. Membrane bound storage structures, contains fluid 2. Types: – Food – stores nutrients – Contractile – contract, dispose of excess water – Plant central vacuole much larger than animal Vacuoles Centriole Centrioles 1. Animal cells ONLY! 2. Organize cell structure during division 3. 2 short cylinders of microtubules Part of cytoskeleton Cytoskeleton • Provides shape and support • 3 structural elements – Microfilaments (Actin)- 2 strands intertwined; POLAR – can be used in cell movement, muscle contraction, cell division – Intermediate fibers – NONPOLAR - anchor nucleus and other organelles in place, maintain shape – Microtubules – hollow tubes made to maintain cell shape, hold organelles in place, POLAR – used in movement of chromosomes during division & organelles (like a railroad track) via the use of kinesins Plastidfound in plants and algae. Contain pigments used in photosynthesis Amyloplast- plant specific, no pigment. Function: synthesis and storage of starch Chromoplast– responsible for carotene(orange), xanthophylls (yellow) and red pigments.