RNA and Protein Synthesis Chapter 10-2 and 10-3 (pg. 190-197) STUDY GUIDE Biology **This study guide is meant as a GUIDE. It is not a substitute for studying the notes, learning the vocabulary or completing the review sheet (if required). ** DNA Structure- Double Helix Function- Contains genetic material-genetic code Nitrogen Bases- A, T, C, G Sugar- Deoxyribose RNA Structure- Single Helix Function- Transcribes & Translates genetic code into proteins Nitrogen Bases- A, U, C, G 1. Two parts of protein synthesis are: (1) Transcription (2)Translation. (easy way to remember the order – ‘c’ comes before ‘l’ ) 2. In transcription, mRNA is made from specific DNA code. 3. Transcription takes place in the nucleus. 4. Translation takes place in the cytosol. Ribosome translates mRNA (codon) code into protein with the help of tRNA (anticodon). 5. RNA polymerase is the enzyme that assists with transcription. 6. DNA Transcribes into mRNA ex: DNA genetic code: T A C G A T = mRNA - A U G C U A) 7. mRNA is complementary to both DNA and to tRNA. Ex: DNA genetic code:T A C G A T = mRNA - A U G C U A = tRNA – U A C G A U) 8. The beginning of the gene being transcribed is the promoter sequence; the end of the gene being transcribed is referred to as the termination sequence. 9. In RNA, the base thymine is replaced by uracil. 10. A protein is a molecule made up of a specific sequence of amino acids. 11. Transporting amino acids to ribosome for assembly into the needed proteins is the function of tRNA. 12. mRNA carries the instructions for making proteins. 13. The start codon is the three base sequence of mRNA that tells the ribosome to begin translating the mRNA. 14. Transcription is the process by which DNA is used to make mRNA. 15. Proteins are put together in the ribosome. 16. RNA is used directly to make protein from the DNA information because DNA is too big and can’t leave the nucleus. 17. DNA and RNA are different because DNA is bigger and has thymine while RNA has uracil; DNA cannot leave the nucleus while RNA can. 18. Leucine is an example of an amino acid (a building block of protein) that tRNA brings back to the mRNA. 19. The tRNA molecule transfers in new amino acids. 20. RNA is a single helix. 21. An anticodon is complimentary to a codon and if found on tRNA. 22. Translation is part 2 of protein synthesis