Environmental Laboratory Central Analytical Facility - Stellenbosch University Victoria Street, Lombardi Building, Room 3016 The Environmental laboratory undertakes analysis of soil, sediment, rock, water and plant materials for environmental, ecological, geological and agricultural investigations. The laboratory is equipped with a range of sophisticated modern equipment, such as atomic absorption spectrometry, ion chromatography, elemental combustion analysis, UV-visible spectrometry, titrimetry, pH, Redox, electrical conductivity measurements, and X-ray fluorescence spectrometry. Laboratory manager XRF analyst Esmé Spicer Tel: (021) 8084788 Fax: (021) 8084791 Email: spicer@sun.ac.za Soil Analyst Mr M W Gordon Tel: (021) 8084788 Fax: (021) 8084791 Email: mgord@sun.ac.za Water Analyst Me Wernich Foit Food Science Water laboratory Tel: (021) 8084788 Fax: (021) 8084791 Email: foitw@sun.ac.za Sample Preparation Technician Mr H Achilles Tel: (021) 8084788 Fax: (021) 8084791 e-mail: hjachill@sun.ac.za Flame Atomic Absorption Spectrometry (AAS) The lab is equipped with two dedicated AAS instruments: the Varian Spectra AA-250 PLUS and the more modern Varian AA 240FS. The latter is fast sequential, meaning that it can determine more than one element in a sample before moving to the next sample. One instrument is dedicated to trace elements (e.g. zinc, copper, lead) and the other to major elements such as calcium, magnesium, sodium and potassium. Calibrations are performed with certified standard reference materials from Riedel-de Haen®. Detection limits are in the ppm range, depending on the method and matrix. International reference materials are used for precision tests. Sample requirements: liquid samples 20 ml, solid soils 1 kg, plant materials 20-100 g (confirm with Matt Gordon) Some sample preparation is required, prior to the final analysis, for soil and plant samples. This involves drying, crushing, milling, extraction and digestion of the samples. The sample preparation and prepreparation for the ICP-MS laboratory can be undertaken by the laboratory if clients do not have their own facilities. VARIAN AA-240FS Elemental analysis (carbon, hydrogen, nitrogen) The EA Euro 3000 is a compact, fully automatic elemental analyzer for the determination of total wt% C, H and N in soil, plant and solid samples. It is based on innovative turbo-flash combustion technology employing pressurized oxygen injection and represents a quantum leap in the art of dynamic flash combustion as well as high temperature (HT) pyrolytic oxygen and hydrogen analysis for elemental and isotopic analysis. Detection limits are typically 0.01 wt%, depending on the material. Calibration is performed with certified standards from Eurovector® and these are also used for routine quality control. Sample requirements: solid samples 10 g EA Euro 3000 Ion chromatography The DX-120 ion chromatograph is used for a multitude of water-soluble anions in aqeous matrices. The instrument combines efficient isocratic separations with digital conductivity detection to provide highly selective and sensitive analyses of soil water extracts and water samples. This instrument is fully automated allowing a large sample throughput. Typical analytes are nitrate (NO3-), nitrite(NO2-), chloride(Cl-), sulfate (SO42-), Phosphate (PO43-) and fluoride (F-). Detection limits are typically in the ppm range and calibration is performed with certified reference materials from Dionix® and these standards are used for routine quality control. Sample requirements: Liquid 10 ml, solids 20g DX-120 Ion Chromatograph X-Ray Fluorescence Spectrometry (XRF) XRF is ideal for rapid and accurate whole bulk elemental analysis in rock or soil samples. The gas-flow proportional counting detector and scintillation detector, or a combination of the two, are used to cover the elements fluorine to uranium. Major elements are analysed on a fused glass bead and trace elements are analysed on a powder briquette. A wide range of international (NIST®) and national (SARM®) standards is used in the calibration procedures and quality control (precision and accuracy) for both major and trace element analyses. Detection limits for the elements quoted, depending on the matrix (combination of elements present), are typically 0.5 ppm. Loss on Ignition (LOI) is a test used in XRF major element analysis which consists of strongly heating a sample of the material at a specified temperature, allowing volatile substances to escape or oxygen is added, until its mass ceases to change. The L.O.I. is made of contributions from the volatile compounds H2O+, OH-, CO2, F, Cl, S; in parts also K, Na (if heated for too long); or alternatively added compounds O2 (oxidation, e.g. FeO to Fe2O3), later CO2 (CaO to CaCO3). Sample requirements (Milled) Major elements: 5 g Trace elements:10 g Other analyses and services offered to environmentalists Routine determinations of pH, EC (electrical conductivity), redox potential and spectrophotometry are also performed on aquous samples. Other specialized extractions and analysis (acid dissolutions, ammonium-acetate extractions, water extractions, EDTA extractions) of plants, soil, water, effluents and fertilizers can be carried out on request. Detection limits are strongly method dependant. Known certified reference materials are used for pH EC, Redox potential analysis and extractions calibration and routine quality control. Sample requirements: confirm with Matt Gordon Environmental Laboratory Analyses user pricelist (prices quoted per sample, VAT excl for industry projects ) Services offered Standard Soil analysis Method/Range pH, AAS (Na, K, Ca, Mg), spectrophotometer Projects for academics in SA 80 Standard Soil analysis with additional trace elements pH, AAS (Na, K, Ca, Mg, Mn, Fe, Cu, P, Zn), spectrophotometer 100 110 Standard Plant analysis Light elements analysis AAS (Na, K, Ca, Mg, Mn, Fe, Cu, P, Zn) Elemental analyser (C, N, H) 100 70 110 110 Anion analysis (additional R22.00 for Soil samples) Ion Chromatography 60 83 pH or Ec or Redox potential or spectrophotometry pH or Ec or Redox potential or spectrophotometry 20 25 Titrations for carbonates and bicarbonates wet chemical titration 35 50 Walkley Black titration wet chemical titration 55 100 XRF major element fused bead analysis on rocks or soils XRF (SiO2, TiO2, Al2O3, Total Fe2O3, MnO, MgO, CaO, Na2O, CaO, P2O5, Cr2O3, LOI determination) 160 230 XRF trace element pressed pellet analysis on rocks or soils XRF (Zn, Ni, Cr, V, Ga, Ba, Rb, Sr, Y, Nb, Zr, U, Th, Pb, Ce, La, Nd) 130 200 100 120 250 350 XRF major element on pressed pellet (Cu Matte) Cu, Co, Ni, Pb, Fe, S XRF calibration for additional element analysis on XRF method determined by element abundance and rocks or soils (price per additional element) sample type Projects for external clients 90 Environmental Laboratory sample preparation pricelist (prices quoted per sample, VAT excl for industry projects ) Sample preparation pricelist (if samples are not handed in to the laboratory in the form ready for analysis) Projects for Projects for external academics in SA clients Services offered Technique used for sample preparation XRF crushing Jaw crusher 15 30 XRF milling Zibb milling 40 50 XRF fused bead for ICP analysis Fusion disc (milled powder supplied by client) 50 70 Rock/soil acid digestion Digestion in water bath 100 120 Environmental Laboratory Water division The Environmental laboratory serves the water community by offering the following analyses: Basic Drinking water tests Basic Sewage water tests Basic and Specialised microbiological testing (MPN+MUG method, Colilert, Pseudomonas spp., Klebiella spp., Surface counts, Microcystis toxicity / toxins) Eutrophication test package (including Chlorifil a content) Trace and ultra-trace element analysis (ICP Laboratory, Riana Rossouw, tel 3114, rrossouw@sun.ac.za) Water analysis prices Microbiological indicators Test Aerobic colony count Heterotrophic plate count bacteria Total coliforms (Not Drinking Water) Faecal coliforms (Not Drinking Water) Escherichia coli (Not Drinking Water) Total coliforms and E.coli (Not Drinking Water) Pseudomonas spp. Klebsiella Faecal coliforms and E.coli (Not drinking water) Faecal coliforms and E.coli (Not drinking water) Total coliforms and E.coli (Drinking water) Total coliforms and E.coli (Not Drinking Water) Method/Range Plate count agar R2A agar Petrifilm (Membrane filter) Petrifilm (Membrane filter) Petrifilm (Membrane filter) VRB agar + MUG Membrane filtration Membrane filtration Multiple Tube Fermentation (MPN) Multiple Tube Fermentation + MUG supplement (MPN + MUG) Colilert Colilert Price / sample in SA rand, excl. VAT Projects for Projects for external academics in SA clients 20 35 50 80 60 90 60 90 60 90 55 100 60 90 60 90 90 120 110 180 220 140 220 250 Water analysis prices: Physical and Chemical tests Test Ammonium Ammonium Biological oxygen demand (BOD) Chemical oxygen demand (COD) Chemical oxygen demand (COD) Chemical oxygen demand (COD) Chemical oxygen demand (COD) Dissolved organic carbon (DOC) Dissolved organic carbon (DOC) Nitrogen Nitrogen Phosphate (PO4-) Total cyanide Total organic carbon (TOC) Total organic carbon (TOC) Dissolved oxygen (On site service) Turbidity K Na Ca Mg SO42ClNitrate, Nitrite F-, BrConductivity (Ec) pH Alkalinity Total Dissolved Solids Suspended Solids Settleable solids Method/Range 0.01 - 2.00 mg/l 0.20 - 8.00 mg/l 0.5 - 3000 mg/l 4 - 40 mg/l 10 - 150 mg/l 25 - 1500 mg/l 500 - 10 000 mg/l 5 - 80 mg/L 50 - 800 mg/l 0.5 - 15 mg/l 10 - 150 mg/l 0.05 - 5.00 mg/l 0.002 - 0.5 mg/l 5 - 80 mg/L 50 - 800 mg/l All All AAS AAS AAS AAS IC IC IC IC Probe with calibration solution Probe with calibration solution Titration Probe with calibration solution Physical method Physical method Price / sample in SA rand, excl. VAT Projects for Projects for external academics in SA clients 40 60 40 60 70 90 60 100 60 100 60 100 60 100 100 200 120 180 70 100 45 55 70 90 80 200 100 200 120 180 45 70 16 25 15.00 15.00 15.00 15.00 50.00 50.00 50.00 50.00 50.00 100.00 15.00 15.00 30.00 15.00 45.00 15.00 20.00 20.00 50.00 20.00 70.00 23.00 Water analysis prices: Eutrophication tests Test pack for Eutrophication Total Organic Carbon (TOC): Dissolved Organic Carbon (DOC): Total Nitrogen (N) Total Phosphorus (P) Dissolved Oxygen pH Conductivity Temperature Aerobic plate count Chlorophyll a content TOTAL US Other Other academics academics Incl. VAT Industry Industry Incl. VAT R 477.00 R 540.00 R 615.60 R 765.00 R 872.10