Research Article Summary
BIOL 501-Senior Project
Author, Title,
Journal, Year,
Volume, Pages
40-Acetoamido-4-hydroxychalcone, a chalcone derivative, inhibits glioma
growth and invasion through regulation of the tropomyosin 1 gene
Bo Mi Ku a, Hyung Won Ryu b, Yeon Kyung Lee a, Jinhyun Ryu a, Joo Yeon Jeong a, Jungil Choi a, Hee Jun Cho c,
Ki Hun Park b,⇑, Sang Soo Kang
Key Terms
Introduction/
Purpose
Research
Question/Hypothesis
Methods
Results
Conclusion/Discussion
Malignant gliomas are the most
common primary malignancies
in the brain, and are
characterized by widespread
invasiveness, angiogenesis,
and necrosis
Although chemotherapy using
temozolomide has recently
been shown to improve
survival in glioblastoma, the
prognosis of gliomas remains
very poor.
The major reasons for
therapeutic failure are the
invasiveness and angiogenesis
associated with these tumors
invading glioma cells or glioma
stemcells cause glioma
recurrence
Therefore, identification of new
therapeutic agents that can
inhibit glioma invasion and
angiogenesis

is of great importance and
significance
Chalcones are considered the
precursors of flavonoids that
are
widely biosynthesized in
plants, and possess two
aromatic rings

joined by a three-carbon
a,b-unsaturated carbonyl
system
4-hydroxychalcone (AHC), inhibited
the invasiveness of
glioma cells through regulation of the
actin cytoskeleton and suppressed
the angiogenic activity of endothelial
cells. Moreover, we
observed that AHC reduced the
growth of human glioma cells
inoculated into nude mice. Taken
together, our results suggest that
AHC might have therapeutic activity
against glioma through
inhibition of cell invasion and
angiogenesis
 invasion assay
 transwell
migration assay
 soft agar gel
colony formation
 western blot
 siRNA infection
 tube formation
assay
 wound healing
assay
 MTT assay

AHC inhibits cell invasion and
migration in glioma cell and
endothelial cell
AHC reduces U87MG cell
invasion and migration through
expression of tropomyosin
PKA activation is required for
AHC-mediated tropomyosin
expression in U87MG cell
3.4. AHC inhibits glioma cell
growth in vivo
Here, we show that the synthetic chalcone
derivative AHC inhibits glioma cell proliferation,
migration, and invasion through regulation of
tropomyosin expression.
The aim of this study was to identify
an effective chalcone
derivative that has anti-cancer
activity against glioma
These data imply that AHC is a potential
therapeutic for
treatment of glioma and that it is relatively nontoxic to mice
we have shown that AHC reduces glioma cell
invasion,
migration, and growth at a low concentration. We
provide
evidence that this effect involves, at least in part,
regulation of
production of the actin binding protein,
tropomyosin
the synthetic chalcone derivative AHC might be a
promising chemotherapeutic agent for gliomas,
and that further
study of this compound is warranted