RAPID DNA:
PILOT STUDY FOR LAW ENFORCEMENT
Michael P. Garvey, Jr, PhD
Director, Office of Forensic Science
Philadelphia Police Department
Stephanie Stoiloff
Senior Police Commander, Forensic Services Bureau
Miami-Dade Police Department
Jan Girten
Director, Forensic Services
Colorado Bureau of Investigation
Rapid DNA
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Automated DNA process
Fieldable platform
Non-expert user
90-120 minute swab to answer
Reference or Evidence
Is it us against them?
We need to start somewhere…
Law Enforcement Organizations
Philadelphia Police
Miami Dade Police
Colorado Bureau of Investigation
Rapid DNA Vendors
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GE DNAscan™ Rapid DNA Analysis™ System
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IntegenX® RapidHIT® Human DNA Identification System
Validation & Pilot Studies
Internal Validation (100 items)
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Sensitivity
Repeatability
Reproducibility
Controls
Precision
Mixture
Mock Evidence
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Reference
Limited Forensic
Operational Pilots
 Reference samples
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Lab based
Field based
General purpose vs. targeted
collections
Arrestee collections
Limited forensic evidence
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Confirmatory testing
Sample size
Intelligence value
Goals
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Law Enforcement and Forensic evaluation of current
state of technologies through two independent
assessments involving multiple labs
Data shared with LE and Forensic communities to
allow informed decisions
Cost comparisons
Operational scenarios
Development of LE and forensic protocols
Work with other labs who are participating in efforts
to complete testing for NDIS approvals, etc.
Current Status
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Philadelphia Police Department
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DNAscan (GE Healthcare)
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RapidHIT (IntegenX)
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Complete: delivered, installed, training
In progress: internal validation of reference and forensic samples
Pending: Operational pilot program
Miami Metro Dade Police
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Complete: delivered, installed, training, internal validation
Pending: evaluation of forensic cartridges and operational pilot program
Pending delivery and installation
Colorado Bureau of Investigation
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Pending delivery and installation
The purpose of these studies is
to provide an evaluation of
each of the leading vendors of
Rapid DNA.
Each study/pilot is an
independent assessment. It has
not been designed or
performed as a head to head
comparison. Any comparisons
will be left to the informed
consumer.
Philadelphia Office of Forensic Science
DNAscan Evaluation
April 2015
Internal validation summary of samples tested
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Promega PowerPlex™
19 runs, 95 samples from 20 known donors
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60 single source samples
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Sensitivity
Reproducibility
Repeatability
NIST traceable
25 mixture samples
10 blanks
DNAscan™ :References
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DNAscan RFID swab used for all samples
60 reference buccal swabs from 20 known donors
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55 generated a full 16 locus profile without human intervention.
4 generated a 16 locus profile with minimal human intervention
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1 sample generated partial profile at (1 swipe)
Mixtures
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Human intervention was limited to exporting data then importing into
GeneMapper® ID-X v1.2 software for analysis.
1:1 -- all flagged as mixtures
5:1 -- 1 sample in each mixture set flagged as mixture, others only major
19:1 – all called as major contributor only
Controls
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All as expected
6 swipes
3 Swipes
1 swipe
DNAscan™ : Forensic
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Pending
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Evaluation of the forensic (low concentration) cartridge
Philadelphia Office of Forensic Science
RapidHIT Evaluation
April 2015
Test Plan and Success Rates
Sample Type
Positive Control
Negative Control
Sensitivity
Description
NIST traceable swabs
Blank wells
Cotton swabs - 10, 5, 2, 1 swipes; 1 touch
Precision
Accuracy and
Repeatibility
Allelic ladders
Mixtures
Semen
Cigarette Butt
Water Bottle
Blood on Denim
Bode Buccal Collector punches (6 mm)
Cotton Swabs
Blood punches (6 mm)
Blood on swab (5 ul)
19:1, 9:1, 5:1, 1:1, 1:5, 1:9, 1:19 (total vol 5 ul
blood)
Neat semen on swab (1 ul)
Cutting
Cotton Swab
Cutting on Denim (5 ul)
Preliminary Data: Validation still in progress
RapidHit # RapidHIT %
of Samples loci generated
2
100
7
0
10
99.1
±0.05
17
bp std. dev.
6
9
4
2
97.1
99.5
70
100
7
1
1
1
1
NA
100
72
95.8
100
5uL Blood on Denim (direct insertion)
1uL Semen on Swab
Cigarette Butt Filter Paper (direct
insertion)
Plastic Water Bottle (swabbed)
Future
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Complete the studies
Present to LE and Forensics
Develop recommendations, training, and
protocols
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Cost analysis
Technical analysis
Operational analysis