Chapter 8 Section 2 - Photosynthesis Photosynthesis Light energy is trapped and converted into chemical energy during photosynthesis. Cellular Energy Overview of Photosynthesis Purpose of Photosynthesis To convert light energy into an energy that organisms can use. Cellular Energy Overview of Photosynthesis Purpose of Photosynthesis Plants make sugars through photosynthesis, an energy that all life on earth can use. Protists such as algae and certain bacteria can use photosynthesis as well. Without photosynthetic organisms, there would be no life on earth! Cellular Energy Photosynthesis The Reactants of Photosynthesis are: Light – from the sun Carbon Dioxide (CO2) – from the air Water (H2O) – from the roots The Products of Photosynthesis are: Glucose (C6H12O6) - sugar Oxygen (O2) Cellular Energy Overview of Photosynthesis Photosynthesis occurs in two phases: Light-dependent reactions (Light reactions) Light-independent reactions (sometimes called dark reactions or Calvin cycle) Overview of Photosynthesis Occurs in the chloroplasts of plant cells. Cellular Energy Photosynthesis Phase One: Light Reactions Takes place in the thylakoids. Chloroplasts Chloroplasts are the organelles that capture light energy. Disk-shaped Cellular Energy Photosynthesis Phase One: Light Reactions Chloroplasts 2 compartments: Thylakoid: membranes that contain stacks called grana; where light reactions take place. Photosystems I and photosystems II contain lightabsorbing pigments and protein complexes important in the light reactions. Stroma: Fluid-filled space outside grana. Where light-independent reactions take place. Cellular Energy Photosynthesis Phase One: Light Reactions Pigments • Light absorbing molecules found in thylakoid membranes. • Different pigments absorb different wavelengths of light and reflect others. • Chlorophylls are the major light-absorbing pigments in plants. • Chlorophylls absorb most in blue-violet region of visible light spectrum and reflect light in green spectrum. Chloroplast Pigments • Chloroplasts contain several pigments: – Chlorophyll a – Chlorophyll b – Carotenoids Cellular Energy Photosynthesis Basics of the Light Reactions The absorption of light is the first step of photosynthesis. When light strikes a thylakoid in a chloroplast, energy is transferred to and excites electrons in chlorophyll. https://www.youtube.com/watch?v=YeD9idmcX0 w Section 2 Cellular Energy Photosynthesis Basics of the Light Reactions Energized electrons are used to break down (split) water molecules. o Oxygen is released through openings on the underside of the leaf called stomata. o Activated electrons are passed along a series of molecules along the thylakoid membrane, the electron-transport chain. This generates energy in the form of NADPH. o Energy produced from protons (H+) is used to synthesize ATP. Cellular Energy Photosynthesis Basics of the Light Reactions Energy stored is stored temporarily in energy carrying molecules, ATP and NADPH that will be used in the Calvin cycle (light-independent reaction). https://www.youtube.com/watch?v=hj_WKgnL6MI https://www.youtube.com/watch?v=joZ1EsA5_NY Review: ATP CYCLE • Occurs continuously in cells • About 10 million new ATP molecules are made in every cell every second!!! 2. Releasing Energy Energy released by breaking bonds – used to power cells 1. Stored Energy Energy stored in chemical bonds. 4. Making ATP Energy released by other chemical reactions and processes can be used to bond a phosphate to ADP to make ATP ADP + P 3. Energy Depleted ADP has less chemical energy than ATP Phase Two: The Calvin Cycle (Light-Independent Reactions) A series of enzymeassisted reactions in which chemical energy stored in ATP and NADPH is used to synthesize carbohydrates such as glucose. Phase Two: The Calvin Cycle (Light-Independent Reactions) Does not require light. Occurs in the stroma of chloroplast. Requires CO2. Uses ATP and NADPH as fuel to run. Makes glucose sugar from CO2 and hydrogen. Plants use sugars formed during the Calvin cycle as a source of energy and to form starch, which stores energy, and cellulose for structure of cell wall. https://www.youtube.com/watch?v=E_XQR800AgM Chloroplast Light Stroma NADP Stack of thylakoids ADP +P Light reactions Calvin cycle Sugar used for Cellular respiration Cellulose Starch Other organic compounds Cellular Energy Photosynthesis Factors That Affect The Rate Of Photosynthesis Light intensity If you increase the amount of light, then photosynthesis will increase up to a point. Temperature CO2 concentrations Water Cellular Energy Alternative Pathways Many plants in extreme climates have alternative pathways for photosynthesis. C4 plants In hot, dry environments where light energy is plentiful but water is scarce. Sugarcane Corn Some grasses Cellular Energy Alternative Pathways CAM plants Water conserving plants. Open their stomata at night. Thick cuticle. Cacti Orchids Pineapple