Sanitation Monitoring for the Craft Brewing Industry Bill Hallagan – Territory Manager Rob Soule – Product Manager Agenda 1. ATP Technology 2. Sanitation Monitoring for Craft Brewing Industry 3. AccuPoint Advanced System 4. Acumedia 5. Monitors, Ampoules & Allergens 6. Neogen’s Craft Brewer Program What is ATP? • ATP stands for Adenosine triphosphate. • It is the biological energy found in all living cells. • ATP is used as an indicator for cleanliness. • Light output is measured by reader in Relative Light Units (RLU’s). • ATP on a surface = cleanliness of a surface. Why Do We Test for ATP? • Bacteria, yeast and molds; pathogens: Listeria, E. coli, Salmonella. • The cleaner the surface, the less likely it is contaminated with spoilage organism or pathogens. • Real time results, under 20 seconds, allows for corrective action. • The Less ATP on a surface the cleaner the surface. Sanitation Monitoring for Craft Brewers • Significant resources (time, money & energy) are used to clean & sanitize brewing equipment. • But, how do you know it’s clean? • ATP technology can be used as a tool to verify cleaning program are effective. • Also, ATP testing can be used in conjunction with other testing (allergen, media) as part of a comprehensive program. • Benefit: improve product quality (i.e. taste) • As your partner, Neogen can help get your program started. AccuPoint Advanced Take your program to a new level of clean. System Components 1. Samplers • • Disposable devices used to recover ATP 3 Types: Surface, Water & Access 2. Reader • • • Detects the amount of ATP present Results reported in Relative Light Units (RLU) Only ATP system with RFID Technology 3. Software • • • Create test plan of sampling sites Analyze data & generate reports Track ATP levels over time AccuPoint Advanced Samplers Best in class sampler for recovery & release of ATP & biofilm breakthrough. Only Neogen has this design! • Liquid stable enzyme solution for increased precision and sensitivity. • Faster reaction - ATP immediately detected • Single step activation • No loss of signal Superior Recovery Engineered from the ground up to be better! More consistent Designed to break open and expose BIOFILMS. Superior Recovery Engineered from the ground up to be better! More consistent Designed to break open and expose BIOFILMS. RLU Guidelines Default thresholds are: • • 0-150 RLU (Pass) 300+ RLU (Fail) Through testing, different surface types may require different threshold levels. 300+ RLU 151-299 RLU 0-150 RLU Flexibility to adjust thresholds based on collected data. Fail Caution Pass Recommended Test Sites • Cellar • • Yeast Tanks/Prop System Fermenter/Bright Tank • Packaging • Bottling Line • • • • • Rinse supply water Rinser nozzles Filler valves Crown track/chute Crowner chucks & transfer plate Recommended Test Sites • Can Line Filler valves • • • Bubble breaker Under cover gasser (seamer) Seamer heads and end transfer plate • Keg Line • • In Line Sampler Port Keg Filler Testing Frequency • Daily ATP tests after CIP. • Reports can help establish baseline RLU values. Benefits of ATP • Routine monitoring with verification ensures cleaning protocols are effective. • Periodically reviewing data drives continuous improvement. • Complements allergen testing. • Awareness of testing by personnel helps modify behavior & improves attitude toward cleaning. • Clean environment = better product quality = higher sales and product adoption. Media Acumedia Products • Universal Beer Agar (#7474) • Lactobacilli MRS (#7543) • W-L Nutrient Agar (#7488) • Malt Agar (#7456) • Y & M Agar (#7525) Filtration, Ampoules & Allergens Filtration - .45um monitors Ampoules – 2mL liquid media Allergens – 10 minute test, lateral flow World Class Products & Support • Extensive Training • • • On-Site GoToMeeting (Presentations & Troubleshooting) LabLive (NeoCare) • Technical Support • • • Issue Resolution Annual calibrations Replacement readers – sent within 24 hours • Superior Customer Service experience! Craft Brewers Program Craft brewer Program Many of our brewers already order Dehydrated Culture Media from us. By adding any other product line for example Accupoint ATP system You qualify for additional discounts on BOTH. How have we been able to help Michigan Craft Brewers? Purchases of this equipment are normally expensive for start up brewers. This program features a FREE rental on the Accupoint reader, all you just buy are the samplers. All that we ask is for you to have an active program. And for that you need to test regularly . Use of at least 1 box (100) or more of samplers a month gets you the free rental ! Thank you!