Milsana® Fungicide Nick Macris Director, Business Development [email protected] (727) 742-2571 (mobile) (530) 750-2800 (office) www.marroneorganics.com Milsana® Fungicide Overview Source: Extract from giant knotweed Reynoutria sachalinensis (REYSA). Target pathogens: Milsana® effectively controls powdery mildew, gray mold, rust, and bacterial blight on ornamental plants and edible crops. Mode of Action: Induction of plant defense responses. – – – – – Reactive oxygen species Enzymes of the phenolic pathway Lignification of cell walls Papilla formation Phytoalexins Has EPA crop and non crop registrations as biochemical biopesticide. California - non crop label only. Milsana® Label Recommendations Apply at the rate of 0.5-1.0% in 50 to 100 gallons of water per acre. Add 0.02% wetting agent such as Nu-Film P. Use the spray mixture preventatively at the 4-6 leaf plant stage and repeat spraying every 7-14 days depending on disease pressure. Milsana® on Ornamentals Begonias Lisianthus Salvias Crape myrtle Petunias Snapdragons Freesias Poinsettias Zinnias Gerbera Roses Diseases Powdery Mildew (Oidium spp) Gray Mold (Botrytis cinerea) Rust (Puccinia antirrhini) Milsana® on Food Crops Cucumbers Melons Squash Grapes Peppers * Strawberries Lettuce Pumpkins Tomatoes Diseases Powdery Mildew (Sphaerotheca spp, Erysiphe cichoracearum ) Gray Mold (Botrytis cinerea) Bacterial blight (Xanthomonas campestris)* Summary of Milsana® Activity Summary of REYSA Extract Activity on Various Edible Crops and Diseases, 1994-2001 Crop / Plant Cucumbers Diseases Sphaerotheca fuliginea Induction Activity +++ Squash S. fuliginea +++ Melons S. fuliginea +++ Grapes Uncinula necator +++ Peppers Xanthomonas spp. ++ Pumpkin S. fuliginea ++ Lettuce Erysiphe cichoriasearum ++ Strawberries S. macularis ++ Wheat Erysiphe graminis ++ Beans Botrytis cinerea Sclerotinia + (-) Field Data Grape powdery mildew trial, (W.D. Gubler, Univ. of California, Davis, July 2000) Trt# Product, sequence Severity % Incidence % (among 24 combinations tested) 1 Untreated 62.0 a 100 a 2 Serenade® WP + Rally® WP, 7x 4.0 b 40.0 b 3 Milsana®, 7x 2.2 b 28.8 b 4 Mankocide® + Microthiol® 80 DF, 5x and Sulfur 80 DF, 7x 2.0 b 32.5 b 5 Sulfur 80 DF 1x followed by Procure® 50 WS + Sulfur 80 DF,7x 1.3 b 21.3 b 6 Serenade® WP, (low) 13x 1.2 b 33.1 b 7 Abound® 2.08 SC, 7x 0.5 b 13.8 b Field Data Botrytis disease ratings and yield of “Trust” Tomatoes (Central Mississippi Research Center, 2003) Treatment Untreated Control Milsana® Elevate® 50WG Switch® 62.5 WG Serenade® Scala® Disease rating (0-100%) 53.3 a 26.7 b 16.7 b 26.7 b 20.0 b 53.3 a Marketable yield per plant (lb) 9.6 a 11.4 b 11.2 b 11.4 b 10.7 a 10.7 a Regulatory/Toxicology Current EPA registration for crop and non crop (ornamentals) uses. CA non crop label only (MOI will submit crop label by the end of 2007). More targets will be added (e.g. tomato, turf). All other crops and diseases are opportunities for IR-4 priorities. MOI is working on an organic formulation. Potential IR-4 Projects Test Milsana® as part of IPM programs in ornamentals, vegetables, fruit, vines (Botrytis, mildews, bacterial diseases. Other diseases ? Test Milsana® on turf diseases. Test efficacy against other pathogens, such as oomycetes (Phytophthora spp. and downy mildew).