Study on application of Chinese
Biological Pesticide
Speaker: Kelley Zhou
Beijing Multigrass Formulation Co.,Ltd
Overview of biological pesticides
Introduction of popular biological
pesticides
Problem and suggestion of biological
pesticides
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选择性强 Selective
对生态环境影响小 Environmental friendly
产品寿命长 Long life of products
原材料来源广泛 Rich Raw Material
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1. 应用会更加广泛
Extensive Application
2. 市场份额会逐年增加
More Market Share
3.和化学农药共同发展，相辅相促
Coexist with Conventional Pesticides
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Advantages of Chinese Bio-pesticides
1. 几千年的历史经验
Historical experience of
thousands years
2.雄厚的理论和技术基础
Deep theoretical and technical
foundation
3.广泛的原材料来源
Extensive Source of Raw
Material
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Types of active
ingredients
Numbers of
products
Yearly capacity
of formulation
Application
area
• More than
80
• More than
2500
• More than 100
thousand MT
• 0.4-0.5 billion
acres
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Main products in the market
Euphorbia
Stemona
Tripterygium
Croton
Sophora
flavescens
Melia toosendan
Main products in the market
井岗霉素 Validamycin
阿维菌素 Abamectin
赤霉素 Gibberellin
苏云杆菌
BT
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Matrine
Matrine
• Matrine is a kind of alkaloid
extracted from Sophora
flavescens Ait. It can act as
pestidide, fungicide and also
stimulating plant growth and
used for controlling the pests
of Lepidoptera , mites ,etc.
Activities and features of Sophora flavescens Ait extract
1. Insecticidal activity
contact poison firstly, stomach
poison secondly. It can control and
kill pests.
2. Bactericidal activity
It can inhabit fungus, bacterium and
virus.
3. Stimulate plant growth
Amino acids, fatty acids and other
substances in it can stimulate and
regulate the growth of crops.
Field trial collection of Matrine product
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Test Condition
Test place: Rio Grande do Sul in Brazil
Temperature: 17,9°C - 23,8°
Humidity: 73%---87%
Test period: October 2008
Test design
Treatment: 0.5% matrine SL 1000 times , 40% omite EC 1000
times and water control
Dosage: 1kg/ha for each concentration
Application times: one application for each treatment
Efficacy calculation
We check the pests alive and dead number after 1 day,
2days, 3days and 5 days.
The mites observed on the leaf
Mites zoomed in
Three days after application of
40% Omite 1000times
Three days after application of
0.5% matrine SL 1000times
controlled by 0.5% matrine SL
Time
Control
0.5% matrine
1000 times
40% Omite EC
1000 times
Water comparision
1day
2 days
3 days
5days
26%
72%
98%
100%
49%
90%
75%
63%
0
4%
4%
10%
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Test Condition
Test place: an indoor laboratory of Bogota City in Colombia
Test period: August 2006
Test design
Treatment: 0.3% matrine 1000 times(1kg/ha), 20% methomyl
1500 times(0.75kg/ha) and water control
Application times: one application for each treatment
Efficacy calculation
We check the pests alive and dead number after 1 days, 3
days, 7 days and 10 days. For each check, we chose 10 plants
randomly and for each plant we checked 10 leaves.
It is in threshold before application
The bud covered by aphides
3 days after application of 0.3% matrine1000 times
Treatment
Pest
density
before
application
1 day after
application
3 days after
application
7 days after
application
10 days after
application
Density
Efficacy
Density
Density
Efficacy
Density
Efficacy
Efficacy
0.3% Matrine
1000 times
13.8
0.1
99.1
0
100
0.1
99.8
0.3
99.4
20%
methomyl EC
1500 times
8.9
0.2
98.5
0.1
99.7
0.3
99.1
0.6
98.1
Water
control
12.2
18.1
0
42.1
0
46.4
0
43.0
0
Oligo Saccharin
Based on halobios，
advanced technology
extract the lowmolecular
carbohydrate
The animals & plants
self immune
formulation
Supply nutrition
Prevent and cure
disease of crop
Human medicine,
plants care, plants
nutrition
Function
Improve solid
environment
Induce plants to
generate diseaseresistant hormone
and active cells
Provide plants
nutrition
Target diseases:
Downy Mildew, Fusarium Wilt, Verticillium Wilt, Ring Spot,
Anthracnose, Angular leaf spot, soft rot, Root knot nematode, etc.
Product: 3% Oligo sacchrin
Contrast: metalaxyl
Disease：downy mildew
Level of disease： moderate
Crop： rose
Date： 06/12/2007-21/12/2007
Method： foliar spray, 7 days interval, 3 times
Dosage ：1.5L/ha.,dilution rate is 600
Person in charge: Hernando
Location：Colombia
The disease was controlled after applied
with 3% Oligo saccharin
The disease was still alive after applied
with metalaxyl.
Suggestion: When crops infected by downy mildew, dilute
Baiclean with 600 time, foliar spray. 7 days interval, 3 times.
Use of Oligo saccharin at the beet crop
 Objective: To evaluate performance in the control of nematode
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Products used: 3% Oligo saccharin x Nemix(nematocide) x Control
Technical Responsible: Ricardo Martins
Property: Farm Canada
City: Uberaba, State of Minas Gerais
Producer: Claudio Carvalho Ottoni
Date of installation of test: 18/12/2008
Pontecial of the producer:
• Carrot 650 ha to 700 ha / year
• Beet 120 ha / year
 Culture:
• Variety: Beet Ferry Morse ：Ferry Morse
• Destination of output: Fresh Consumption
 Date of sowing / transplanting: 18 / 12 / 08
 Date of collection: 08 / 03 / 08
• Planting spacing: plots has a width of 1.75 m with 4 double rows, where we have
20 plants in double line, obtaining a final stand of 457,000 plants / ha.
 Technique of cultivation: Irrigation Central Pivot.
 Methodology: The trial was to test an area of 1,000 m2 and the control is
in the central area.
 The applications were as follows:
– 1st application 03 days after sowing;
– 2nd application 07 days after germination;
– 3rd application 20 days after germination;
– 4th application 40 days after germination;
– 5th application 60 days after germination.
• All applications were isolated, because we do not know the compatibility,
and used for all applications a flow of 400lts of water / ha with a dosage
of 800ml/ha.
• Valuation:
• - 1st Rating: 19/01 - after thinning
- 2nd Rating: 17/02
Control (without nematicide)
NEMIX (Standard)
One Application
3% oligo saccharin
Four Application
Extracted
from
orange pill
15%
Bioflavonoid
Complex
Control
bacterial
and fungal
diseases
Crops:
vegetable,
horticulture
and fruit.
Test information
Product
Contrast
Disease
15% Bioflavonoid Complex
Kresoxim-methyl
Powdery mildew
Disease level
Moderate
Crop
Method
Rose
23/03/2009-14/04/2009
Foliar spray
Dosage
0.75-1L/ha., dilution rate1000-1500
Date
Person in charge
Location
Murad
Ethiopia
Overview of rose plantation
When the buds of rose infected by powdery mildew, they would
not open or even they will not come into flower.
New leaves sprouted after applied with 15% Bioflavonoid Complex. After
recovery, the white powder was removed, there were yellow spot on the leaves.
Test result
Product
Dosage
（fungicide/water）
/dilution rate
Disease
Crop
% efficacy
15%
Bioflavonoid
Complex
1.25 cc/L
800 times
Powdery
mildew
Rose
72%
15%
Bioflavonoid
Complex
1.5 cc/L
600 times
Powdery
mildew
Rose
85%
Kresoximmethyl
1.5 cc/L
600 times
Powdery
mildew
Rose
76%
1. 认知的误区(acknowledge)
Problems
2.生产污染(Pollution)
3.使用方法(Application)
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1. 发掘历史沉淀(Histrorical resource)
2. 重视制剂的研发(Formulation)
3. 加大登记的投入(registration)
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